Thermo Fisher Scientific Easion Quick start guide

Brand
Thermo Fisher Scientific
Model
Easion
Type
Quick start guide
System priming and rinse
1. Verify that the pump is turned o.
2. Connect the pump eluent line to a bottle containing
18 MΩcm resistivity or better ASTM Type 1 deionized
(DI) water.
3. Disconnect the waste line from the luer fitting on the
secondary (left) pump head.
4. Connect a 10 mL syringe to the luer fitting.
5. Open the waste valve on the secondary pump head
by turning the knob one-half turn counterclockwise.
6. Draw the syringe back to begin removing air from the
flow path. When a small amount of liquid enters the
syringe, remove the syringe from the luer fitting, and
reconnect the waste line.
7. With the waste valve open, click Prime on the Thermo
Scientific™ Dionex™ Chromeleon™ Chromatography
Data System (CDS) ePanel. Wait until no air bubbles
are exiting the pump waste line.
8. Turn the pump o and close the waste valve.
9. If the columns are installed on the system remove
before flushing the system. Pure water should not be
run through the IC columns.
10. Turn on the pump, setting the flow rate to 1 mL/min,
and flush the system for 15 minutes.
11. Prepare the eluent following the directions from the
concentrate solution or from your method.
Suppressor/column installation and conditioning
•The columns must be placed with the guard column
before the analytical column and the flow direction
following the arrows on the column labels.
•Pump eluent through the columns at your method flow
rate for about 30 min, directing the eluent exiting the
column to a waste container.
•If the suppressor is not installed and connected to the
system the suppressor will need to be hydrated before
use, while the columns are being equilibrated.
To hydrate the suppressor
• If running anions: Using a disposable plastic syringe, push
3 mL of 200 mN H2SO4 through the “Eluent Out” port and
5 mL of 200 mN H2SO4 through the “Regen In” port.
• If running cations: Using a disposable plastic syringe, push
3 mL of 200 mN NaOH through the “Eluent Out” port and
5 mL of 200 mN NaOH through the “Regen In” port.
•Allow the suppressor to sit for 20 min to ensure complete
hydration before installing it in the system.
Easion Quick Start Guide
Use this quick start guide to help get your Thermo Scientific™ Dionex™
Easion™ IC system up and running quickly and easily.
Conductivity cell
Suppressor
Separator column
Pump heads
Pressure transducer
Injection valve
Tubing chase
Guard column
Backpressure tubing
1
2
3
4
5
6
7
8
9
1
2
3
4
5
6
7
8
9
Preparing to run the system
• If using displacement chemical regeneration (DCR) mode,
connect the tubing from the separator (analytical) column to
the “Eluent In” on the suppressor. Connect the “Eluent Out”
to the conductivity cell. From the conductivity cell, connect
the tubing to the inlet of the regenerant bottle. The tubing
from outlet of the regenerant bottle is connected to the
“Regen In” on the suppressor. Finally, the “Regen Out” goes
to waste. NOTE: when using DCR mode, the regenerant
bottle must be filled to the top of the rim.
• If using external regenerant mode, such as using an
auxiliary pump, connect the tubing from the separator
(analytical) column to the “Eluent In” on the suppressor
and the “Eluent Out” is connected to the conductivity
cell. The outlet of the regenerant bottle is connected to
the “Regen In” on the suppressor and the “Regen Out”
is connected to waste.
• Equilibrate the system at the application flow rate
for about 30 min and monitor the baseline until the
background conductivity is <30 μS/cm on a system set
up for anion analysis or <2 μS/cm for cation analysis.
• Start sample sequence in Chromeleon CDS.
Dionex Easion components
For Research Use Only. Not for use in diagnostic procedures. © 2021 Thermo Fisher Scientific Inc. All rights reserved.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. XX000029 0621
For the full user manual, technical specifications and
product information visit thermofisher.com/easion
Eluent out
to cell
Eluent in from column
Regenerant out
to waste
Conductivity Cell
(in DS5 Detection Stabilizer)
ACRS 500
Regen Out
Regen In
Eluent Out
Eluent In
Used eluent out to
regenerant reservoir
Regen bottle out
Regen in
Regenerant in
from reservior
Anion
Regenerant
Used
Eluent
Eluent out
to cell
Eluent in from column
Regenerant out
to waste
Conductivity Cell
(in DS5 Detection Stabilizer)
CCRS 500
Regen Out
Regen In
Eluent Out
Eluent In
Used eluent out to
regenerant reservoir
Regen bottle out
Regen in
Regenerant in
from reservior
Cation
Regenerant
Used
Eluent
To pump inlet
From cell outlet
To cell inlet
Backpressure Coil
(1or 2, depending
on flow rate)
Conductivity Cell
(inDS5 DetectionStabilizer)
Pulse Damper
CRS 500
Injection
Valve
To WasteTo Waste
Pump
Heads
Pressure Transducer
Regen Out
Regen In
P
C
L
L
W
SSample in
Guard
Eluent Out
Eluent In
Eluent
Secondary Primary
Separator
Eluent in
from pulse
damper
To CRSRegen In
Regenerant
Dionex Easion flow schematic
Dionex Anion displacement chemical regeneration (DCR)
flow schematic
Cation DCR flow schematic
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Thermo Fisher Scientific Easion Quick start guide

Brand
Thermo Fisher Scientific
Model
Easion
Type
Quick start guide
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