Introduction 1-7
AmpFlSTR
Profiler PCR
Amplification Kit
Contents
The AmpFlSTR Profiler PCR Amplification Kit contains the PCR
reagents necessary to co-amplify the ten AmpFlSTR Profiler loci. The
kit components are shown in Table 1-2. The recommended storage
temperature for each component is listed in the Product Insert
contained in each AmpFlSTR Profiler kit.
Table 1-2 AmpFlSTR Profiler PCR Amplification Kit Components
Kit Component Volume Description
AmpFlSTR™ PCR Reaction Mix 1.1 mL/tube Two tubes each containing MgCl2,
deoxynucleoside triphosphates (dATP, dCTP,
dGTP, dTTP), 160 µg/mL bovine serum albumin
(BSA), and 0.05% sodium azide (NaN3) in
buffer and salt
AmpFlSTR Profiler Primer Set 1.1 mL One tube of locus-specific 5-FAM-, JOE-, and
NED-labeled and unlabeled primers in buffer to
amplify the STR loci D3S1358, vWA, FGA,
TH01, TPOX, CSF1PO, D5S818, D13S317,
and D7S820, and the gender marker
amelogenin
AmpliTaq® Gold DNA Polymerase 50 µL/tube Two tubes of enzyme with an activity of 5 U/µL
AmpFlSTR Control DNA 9947A 0.3 mL One tube containing 50 pg/µL human cell line
DNA in 0.05% NaN3 and buffer. The genotype
of this female DNA is D3S1358 14, 15; vWA 17,
18; FGA 23, 24; TH01 8, 9.3; TPOX 8, 8;
CSF1PO 10, 12; D5S818 11, 11; D13S317 11,
11; D7S820 10, 11
Mineral oil 5 mL One dropper bottle
AmpFlSTR Blue Allelic Ladder 25 µL One tube of AmpFlSTR Blue Allelic Ladder
containing the following amplified
5-FAM-labeled alleles: D3S1358 12–19, vWA
11–21, and FGA 18–30 (including 26.2)
AmpFlSTR Green I Allelic Ladder 25 µL One tube of AmpFlSTR Green I Allelic Ladder
containing the following amplified JOE-labeled
alleles: amelogenin X and Y, TH01 5–10
(including 9.3), TPOX 6–13, and CSF1PO 6–15
AmpFlSTR Yellow Allelic Ladder 25 µL One tube of AmpFlSTR Yellow Allelic Ladder
containing the following amplified NED-labeled
alleles: D5S818 7–16, D13S317 8–15, and
D7S820 6–15