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Hello! I've reviewed the user bulletin regarding extension primers for the DNA Sequencing System 373A. This document provides guidelines on primer selection, synthesis, and purification to ensure high-quality sequencing data. I can answer your questions about the recommendations for primer length, melting temperature, or secondary structure issues, as well as synthesis and purification methods.
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What should be avoided when selecting primer sequences?
What is a recommended minimum length for primers to ensure goof hybridization?
What is the recommended melting temperature for cycle sequencing primers?
What is the best choice for primer synthesis?