Leica Microsystems EM CPD300 Application Note

Brand: Leica Microsystems

Model: EM CPD300

Type: Application Note

Leica Microsystems EM CPD300 is a critical point dryer designed for drying biological, medical, and industrial samples for electron microscopy. It features a unique closed-loop CO2 system that ensures consistent and repeatable results, even for delicate samples. The EM CPD300 also has a variety of safety features, including a built-in leak detector and an overpressure relief valve.

Application Note

Wrinkled Giant Hyssop Leaf Protocol

related instrument Leica EM CPD300

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Wrinkled Giant Hyssop Leaf Protocol

INTRODUCTION

Species: Wrinkled Giant Hyssop (Agastache rugosa)

Critical point drying of wrinkled giant hyssop leaf with subsequent gold coating and SEM analysis

PROCEDURE

Sample Holder:

Samples were inserted into the 22 mm cover slip holder

FIXATION AND DEHYDRATION

2.5% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.2 14 h

0.1M Sodium Phosphate Buffer, pH 7.2 3x 10 min.

Acetone series: 30%, 50%, 70%, 80%, 90%, 95%, 100% 2x 10 min.

CPD300 auto Program:

Coating:

Gold: 15-20 nm

RESULTS

The leaf of Wrinkled Giant Hyssop

Courtesy of Dr. Guo JianSheng, School of Life Sciences and Technology, Tongji University, China

LNT Application Note - HYSSOP LEAF PROTOCOL

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LIFE SCIENCE DIVISION - NANO TECHNOLOGY LNT

The Leica Microsystems Nano Technology Division’s focus is to

provide the most compehensive product portfolio for the preparation

of biological, medical and industrial samples for investigation in the

Electron and Light Microscope. Excellent Sample Preparation is a

prerequisite for perfect microscopy. Your image starts here!

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Leica EM CPD300 Application Note Wrinkled Giant Hyssop Leaf Protocol ∙ 07/2014 ∙

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Leica Microsystems EM CPD300 Application Note

Leica Microsystems EM CPD300 Application Note

Leica Microsystems EM CPD300 Application Note

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