Chromate 4300 User manual

Type
User manual
CHROMATE
MODEL 4300 MICROPLATE READER
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©Copyright 2008 Awareness Technology Inc. Doc. 4300 10/2008 Rev. E
TABLE OF CONTENTS
1. INTRODUCTION.................................................................................................................................... 1
1.1 APPLICATIONS....................................................................................................................................... 1
1.1.1 Intended Use.................................................................................................................................. 1
1.1.2 Summary of the Instrument............................................................................................................ 1
1.1.3 Principles of Operation................................................................................................................. 2
1.2 WARNING MARKINGS INSCRIPTIONS DAVERTISSEMENT ......................................................................... 3
1.2.1 Safety Symbols Le Symboles de Sûreté.......................................................................................... 3
1.2.2 Safety Terms Terminologie de Sûreté............................................................................................ 3
1.3 SAFETY PRECAUTIONS........................................................................................................................... 4
1.4 OPERATING PRECAUTIONS .................................................................................................................... 7
2. TECHNICAL SPECIFICATIONS......................................................................................................... 8
3. INSTALLATION ................................................................................................................................... 10
3.1 UNPACK INSTRUMENT......................................................................................................................... 10
3.2 PARTS AND CONTROLS........................................................................................................................ 12
3.2.1 Parts of the Instrument................................................................................................................ 12
3.3 INSTRUMENT SETUP & SOFTWARE INSTALLATION .............................................................................. 14
3.3.1 Software Installation.................................................................................................................... 14
3.3.2 USB Port Connection .................................................................................................................. 18
3.4 INSTRUMENT CHECK OUT ................................................................................................................... 19
4. OPERATING PROCEDURES ............................................................................................................. 20
4.1 GENERAL SELECTIONS ........................................................................................................................ 20
4.1.1 Selecting a Plate Format............................................................................................................. 21
4.2 ASSAY MODES .................................................................................................................................... 22
4.2.1 Absorbance Mode........................................................................................................................ 22
4.2.2 Factor Mode................................................................................................................................ 23
4.2.3 Standard Mode ............................................................................................................................ 23
4.2.4 % Absorbance Mode.................................................................................................................... 24
4.2.5 Point-to-Point Mode.................................................................................................................... 24
4.2.6 Polynomial Regression Mode...................................................................................................... 26
4.2.7 Linear Regression Mode.............................................................................................................. 27
4.2.8 Dose Response Mode................................................................................................................... 28
4.2.9 Cubic Spline Mode....................................................................................................................... 28
4.2.10 Cutoff Mode............................................................................................................................... 29
5. CHROMATE MANAGER.................................................................................................................... 31
5.1 OPENING CHROMATE MANAGER ........................................................................................................ 31
5.1.1 ChroMate Manager Toolbar Icons.............................................................................................. 31
5.2 MAIN MENU SELECTIONS & FEATURES............................................................................................... 32
5.2.1 Management Menu...................................................................................................................... 32
5.2.2 Tests Menu................................................................................................................................... 33
5.2.2.1 Read Plate Absorbance.......................................................................................................................... 34
5.2.2.2 Create Assay.......................................................................................................................................... 35
5.2.2.3 Edit Assay.............................................................................................................................................. 38
5.2.3 Lot # Registration Menu.............................................................................................................. 39
5.2.4 QC Tracking Menu...................................................................................................................... 40
5.2.5 Sample Database......................................................................................................................... 42
5.2.5.1 Add to Sample Database........................................................................................................................ 42
5.2.5.2 Modify Sample ID................................................................................................................................. 44
5.2.5.3 Delete Sample ID................................................................................................................................... 44
5.2.5.4 View Log............................................................................................................................................... 45
5.2.5.5 Import Patient Record............................................................................................................................ 46
5.2.5.6 Export Patient Record............................................................................................................................ 47
5.2.5.7 Search Patient Record............................................................................................................................ 47
5.2.5.8 Choose Patient Record........................................................................................................................... 48
5.2.6 Settings ........................................................................................................................................ 49
5.2.6.1 Comm Port, Database, Report Settings.................................................................................................. 49
5.2.6.1.1 Software Settings............................................................................................................................ 50
5.2.6.1.1.1 Start up.................................................................................................................................... 50
5.2.6.1.1.2 Sample Database Settings ....................................................................................................... 51
5.2.6.1.1.3 Report Appearance.................................................................................................................. 52
5.2.6.1.1.4 Report Output.......................................................................................................................... 53
5.2.6.2 Custom Report Settings......................................................................................................................... 54
5.2.6.2.1 Custom Report Sample................................................................................................................... 55
5.2.6.3 Select Language..................................................................................................................................... 56
5.2.7 Maintenance................................................................................................................................ 57
5.2.7.1 Firmware Update................................................................................................................................... 57
5.2.7.2 ChroMate Calibration Lock/Unlock ...................................................................................................... 58
5.2.8 Help............................................................................................................................................. 58
5.3 CHROMATE MANAGER TABS .............................................................................................................. 59
5.3.1 Plate Layout Tab ......................................................................................................................... 60
5.3.2 Assay Calibration Tab................................................................................................................. 62
5.3.3 Add Samples Tab......................................................................................................................... 68
5.3.4 Test Results Tab........................................................................................................................... 71
5.3.5 Report Tab................................................................................................................................... 73
6. TROUBLESHOOTING......................................................................................................................... 76
6.1 ERROR MESSAGES............................................................................................................................... 76
6.2 COM PORT SETTINGS ......................................................................................................................... 78
6.2.1 PC Communication (COM) Port Setting..................................................................................... 78
6.2.2 ChroMate Communication (COM) Port Setting.......................................................................... 80
7. CONTACT INFORMATION ............................................................................................................... 83
8. APPENDIX............................................................................................................................................. 84
8.1 LABORATORY INFORMATION SYSTEM (LIS) ....................................................................................... 84
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 1
1. INTRODUCTION
1.1 Applications
1.1.1 Intended Use
The ChroMate™ 4300 is a laboratory instrument intended for in-vitro diagnostic
use. It is a user-programmable open system with selectable plate formatting,
alphanumeric test naming, automatic interpretation options, duplicate well
options, curve plotting and editing, and flags and error messages.
ChroMate™ is a compact, microprocessor-controlled, multi-purpose photometer
system designed to read and calculate the results of assays, which are read in
microtiter plates.
It is a general purpose instrument intended to be used by trained laboratory
professionals who are capable of selecting the appropriate features and options
for each specific clinical application. Contact your company’s instrument service
provider to arrange for training.
WARNING! FOR IN-VITRO DIAGNOSTIC USE
AVERTISSEMENT! POUR L'USAGE DIAGNOSTIQUE IN VITRO
1.1.2 Summary of the Instrument
This instrument reads monochromatically or bichromatically and has three
models:
Four-filter model (405, 450, 492, and 630 nm)
Six-filter UV model (405, 450, 492, 630, 545, and 600 nm)
Six-filter VIS model (405, 450, 492, 545, 600, and 630 nm)
Substitute filters in the range of 405 nm - 750 nm are available on
special order models.
The ChroMate™ accepts all standard microplates. Trays containing microstrips
may also be read.
Pre-programmed Modes
The basic calculations are permanently stored in memory and include
several single and multi-point equations. Provisions are made in certain
cases for reading specimens in duplicate and/or using the mean reading
in calculations. Each calculation mode is described in detail in Section
4.2-Assay Modes. The following calculation modes are offered: single
point calibration, point-to-point curve fit, cutoff absorbance, multipoint %
absorbance, polynomial regression, linear regression (log & linear), cubic
spline, cutoff absorbance, and multi-point % absorbance.
2 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
User-programmable Memory
ChroMate software allows the operator to create, edit and store assays.
Standard curves are also stored in this memory. Test protocols remain
stored until either changed or deleted by the user.
1.1.3 Principles of Operation
The plate carrier precisely positions each well into the optical path for reading.
Light energy from a lamp is focused by an integral lens, directed through an
aperture, and then passed vertically through the sample. A wheel below the
sample positions the filters so that readings can be taken very quickly at both the
operating and differential wavelengths. (Using bichromatic differential
absorbance values corrects for optical imperfections in the plastic wells and
removes the effects of meniscus and turbidity.) A photo detector converts
transmitted light energy into electrical signals, which are amplified and
interpreted.
The ChroMate™ 4300 Microplate Reader’s optical system provides an
economical and low maintenance design which reads 8 wells simultaneously. A
96 well plate can be read and printed in the absorbance mode in approximately
eight seconds.
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 3
1.2 Warning Markings Inscriptions d’avertissement
1.2.1 Safety Symbols Le Symboles de Sûreté
Symbols that may appear on the product:
Les symboles de sûreté peuvent apparaitre sur le produit:
WARNING Protective Ground CAUTION BIOHAZARD
AVERTISSEMENT La Terre Electrique L’ATTENTION BIOHAZARD
Risk of Shock (Earth) Terminal Refer to Manual Risk of Infection
Risque de Choc Prise de Terre Se Rapportent Risque d’infection
a Manuel
FUSE: For continued protection against risk of fire, replace fuse only with one of
the specified type and current ratings. Disconnect equipment from supply before
replacing fuse.
FUSIBLE: Pour la protection continue contre le risque du feu, remplacez le
fusible seulement par une du type spécifique et des estimations courantes.
Démontez l’équipement de l’alimentation d’énergie avant de remplacer le fusible.
1.2.2 Safety Terms Terminologie de Sûreté
These terms may appear on the product:
Les marques sur le produit:
DANGER
DANGER Le “de marque:
DANGER”
indicates an injury immediately
accessible as you read this marking
indique le risque immédiat de dommages
(assessible tandis que vous lisez la
marque)
WARNING
AVERTISSEMENT! Le “de
marque: WARNING”
indicates an injury hazard not
immediately accessible as you read this
marking
indique qu’un risque de dommages qui
n’est pas immédiat alors vous lisent cette
marque
4 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
These terms may appear on the product:
Les marques sur le produit:
CAUTION
L’ATTENTION “Le de
marque: CAUTION”
indicates a hazard to property, including
the product
indique un risque a la propriété
comprenant ce produit
These terms may appear in this manual:
Les marques dans l’opérateur manuel:
WARNING
AVERTISSEMENT! Le “de
marque: WARNING”
WARNING statements identify conditions
or practices that could result in injury or
loss of life. WARNING indicates an injury
hazard not immediately accessible as you
read this marking.
Ces rapports identifient les conditions ou
les pratiques qui pourraient avoir comme
conséquence les dommages ou les
pertes humaines.
CAUTION
L’ATTENTION “Le de
marque: CAUTION”
CAUTION statements identify conditions
or practices that could result in damage
to this product or other property.
Ces rapports identifient les conditions ou
les pratiques qui pourraient avoir comme
conséquence les dommages a ce produit
ou a toute autre propriété.
1.3 Safety Precautions
To assure operator safety and prolong the life of your instrument, carefully follow all
instructions outlined below.
Read Instructions Please take the time to read this manual carefully before using
this instrument. Review the following safety precautions to avoid injury and prevent
damage to this instrument or any products connected to it. To avoid potential
hazards, use this instrument only as specified. For best results, familiarize yourself
with the instrument and its capabilities before attempting any clinical diagnostic
tests. Refer any questions to your instrument service provider.
Servicing There are no user-serviceable parts inside the instrument. Refer
servicing to qualified service personnel. Use only factory-authorized parts. Failure
to do so may void the warranty.
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 5
Wear Protective Apparel Many diagnostic assays utilize materials that are
potential biohazards. Always wear protective apparel and eye protection while using
this instrument. Always operate this instrument with the aerosol shield lowered.
Follow Operating Instructions Do not use this instrument in a manner not
specified by the manual, or the protection provided by the instrument may be
impaired.
Use Proper Power Cord Use only the power cord specified for this product and
certified for the country of use.
Ground the Product This product is grounded through the grounding conductor of
the power cord. To avoid electric shock, the grounding conductor must be
connected to earth ground. An optional method is to attach a ground strap from the
external grounding terminal on the rear panel of the instrument to a suitable ground
such as a grounded pipe or some metal surface to earth ground.
Warning!
Avertissement!
Warning! To avoid electric shock, the grounding conductor in
the supply cord must be connected to earth ground. An
optional method is to attach a ground strap from the external
grounding terminal on the rear panel of the instrument to a
suitable ground such as a grounded pipe or some metal
surface to earth ground.
Avertissement! Pour éviter la décharge électrique, vous devez
employer la corde de principal courant avec la terre électrique
(prise de terre). Vous pouvez attacher un bus de masse du
terminal a terre externe sur le panneau arrière de l’instrument
dans une pipe fondée des eaux.
Observe All Terminal Ratings To avoid fire or shock hazard, observe all ratings
and markings on the instrument. Consult this manual for further ratings information
before making connections to the instrument.
6 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
Install as Directed Install the instrument on a sturdy, level surface capable of
safely supporting the instrument’s weight 12.471 lbs (5.657 kg). The mounting
surface should be free of vibrations. The instrument does not require fastening to
the bench top.
Provide Proper Ventilation Refer to the installation instructions for details on
installing the product so it has proper ventilation. The instrument should be
surrounded by the following clearances: 46cm on each side, 117cm on top, 15cm in
front, and 18cm in back.
Do Not Operate Without Protective Covers Do not operate this instrument with
covers and panels removed.
Use Proper Fuse Use only the fuse type and rating specified by the manufacturer
for this instrument.
Avoid Exposed Circuitry Do not touch exposed connections and components
when power is present.
Avoid Excessive Dust Do not operate in an area with excessive dust.
Do Not Operate With Suspected Failures If you suspect there is damage to this
instrument, have it inspected by a qualified service person.
Do Not Operate in Wet/Damp Conditions
Do Not Operate In An Explosive Atmosphere
Keep Instrument Surfaces Clean and Dry Solvents such as acetone or thinner will
damage the instrument. Do not use solvents to clean the unit. Avoid abrasive
cleaners; the aerosol shield is liquid-resistant, but easily scratched. Clean the
exterior of the instrument with a soft cloth using plain water. If needed, a mild all-
purpose or nonabrasive cleaner may be used. Use as a disinfectant a 10% solution
of chlorine bleach (5.25% Sodium Hypochlorite) or 70% isopropyl alcohol. Take
special care not to spill liquid inside the instrument.
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 7
1.4 Operating Precautions
NOTE: Insufficient RAM will adversely affect the performance of your instrument.
Therefore, if using Windows
®
ME, the minimum RAM required is 96 MB. For Windows
®
XP, the minimum RAM required is 128 MB.
Avoid lifting, leaning or turning the instrument over when a plate is in place.
Be sure to run a sufficient number of controls in each assay. If controls are not
within their acceptable limits; or if incomplete, disregard test results.
Biohazard Precautions
Warning!
Avertissement!
WARNING: If any materials are overturned during operation,
immediately set the power switch to OFF (0). This material should
be treated as potentially biohazardous. Appropriate cleanup and
disposal of biohazardous waste should be used.
Avertissement! Lors du fonctionnement, si on renverse des
matériaux, coupez immédiatement le courant. Placez le
commutateur électrique a AU LOIN(0). Traitez le matériel
comme biohazardous, utilisant approprie nettoient et des
méthodes de disposition.
8 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
2. TECHNICAL SPECIFICATIONS
The plate moves toward the front and back of the instrument.
When taking an optical reading, the reaction plate automatically positions itself under the
8-channel optical system. A filter wheel with four or six filters rotates below the plate.
Depending on the setup, reports may be displayed or printed to create permanent lab
records and physician reports.
Photometer:
Optical Measurement Range: -0.20 to 4.0 Absorbance Units (A)
Photometric Accuracy: +/- 1% or better (NIST)
Stability: Drift of no more than 0.005A in 8 hours
Warm up: 45 seconds
Light Source: Halogen, lamp saver feature
Wavelengths: Standard four filter: 405, 450, 492, and 630nm
6-filter: 405, 450, 492, 545, 600, and 630nm
Filter options from 405nm to 750nm
Filter Type: IAD hardcoat, 10nm half bandpass
Vessel: Standard 96 well microtiter plates or strip trays
Electronic:
Power Requirements: 100 - 240VAC, 50-60Hz 90W maximum.
All power cords must be approved for the
country of use
PC Connection: USB Port
Microprocessor: eZ80
Speed: Reads absorbance of 96 wells in
approximately 8 seconds
Plate Transport: Stepper motor
Certifications: Pending
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 9
Software:
PC interface with ChroMate™ Manager software – included (PC not
included)
Features: PC interface software (included) with
complete user prompting, error messages, full
plate reporting, automatic interpretation
options, duplicate options, controls locator,
curve plotting and editing
Format: CD-ROM and internet upgrades
Operating Systems: Windows
®
2000, NT 4.0, XP or Vista
Minimum System: Pentium II
®
/333 MHz, 128 MB RAM, CD
drive, SVGA monitor; USB port
Secondary menu options: Create/edit protocols, import/export data, LIS
Calculation modes: Single point calibration, point-to-point curve fit,
polynomial regression, linear and sigmoidal
regression (log and linear), cutoff absorbance,
multi-point % absorbance
Additional Features: User-programmable open system, selectable
plate formatting, reporting
QC options: Store control data, print Levey-Jennings or
QC range plots, calculate SDs
USB port: USB cable provided
Physical
Enclosure: All metal enclosure
Dimensions: 7.5” x 8.0” x 16.5”
(19x20.3x42cm)
Weight: 12.471 lbs (5.657 kg)
Environmental
Operating Temperature: 15 to 35
C
Humidity: Between 10 and 85% non-condensing
Storage Temperature: 10 to 50
C
Altitude: 2000m maximum
Design and instrument specifications are subject to change without notice.
10 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
3. INSTALLATION
3.1 Unpack Instrument
Carefully unpack the instrument, removing it from its plastic bag. Report any damage to
your freight carrier at once. The box will also contain the Operator’s Manual, a power
cord, a USB cable, a replacement lamp, and a USB mass storage device.
NOTE: Retain the original packing material for future use in the event that the instrument
is shipped to another location or returned for service.
Unpacking Checklist
Quantity Description
1 Customer copy of packing list
1 Unpacking instructions sheet
1 Certificate of Conformity
1 Certificate of Quality
1 Instrument Set Up and Operator’s Manual
1 Power cord
1 USB cable
1 Replacement lamp for photometer
1 USB mass storage device
Instrument Mounting and Use
Place the instrument on a flat working surface capable of safely supporting the weight of
the instrument (5.657 kg). Excessive vibration during reading may cause poor
repeatability; thus, a sturdy working surface is required. A clearance of at least 3 inches
(8cm) around the instrument is required to assure optimal ventilation.
Power Switch Position
When installing the power cord the unit should be turned off. Look at the rear panel of
the instrument to check that the power switch is in the OFF position. A diagram of the
rear panel can be found in the Section Parts and Controls.
Power Cord Requirements
Use only the power cord specified for this product and certified for the country of use.
For 110-120 V units used inside the US use a UL listed cord set consisting of a
minimum 18 AWG, Type SVT or SJT three conductor cord, maximum 3 meters (10 feet)
in length, rated 10 A, 125 V, with a parallel blade, grounding type attachment plug.
For 220-240 V units used inside the US use a UL listed cord as above, except rated 250
V, with a tandem blade, grounding type attachment plug. The cord set provided by the
manufacturer meets these requirements.
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 11
Safety Grounding
Do not alter or defeat the safety grounding methods provided. To avoid the risk of electric
shock, the third prong of the AC power plug must be connected to conductive parts
internal to the equipment. Internal fasteners to grounding points are marked by the IEC
417 symbol 5019
. DO NOT loosen or remove these fasteners or connections. An
alternate method of grounding is provided by connecting the grounding terminal located
on the rear panel, to a suitable ground.
To avoid electric shock, the power cord protection ground conductor must be connected
to ground.
Assure Clean Power Availability
The circuit used should be substantially free of large voltage transients (Kilovolt amp
loads) such as large pumps, large centrifuges, refrigerators and freezers, air
conditioners, large autoclaves, ovens, and dryers. The instrument may fail to operate
normally if the power supply is interrupted. If this occurs, turn the instrument off for a
moment. When the instrument is turned back on, it will resume normal operation, but a
standard curve which was not stored in non-volatile memory will be lost.
Fuse Requirements
The fuses are located internally in the instrument; there are two fuses. Fuse number 1 is
located on the main printed circuit board near power connector J4. It is a 4 Amp, medium
acting, 250V, 5 x 20mm, Littlefuse type 0234004 or equivalent. Fuse number 2 is located
on the power supply. It is a 2.5 Amp, fast acting, 250V, 5 x 20mm, Littlefuse type
21602.5P or equivalent.
Fuse failure is a very rare occurrence and may indicate malfunction of the equipment
requiring service by qualified personnel.
Warning!
Avertissement!
FUSE
FUSIBLE
Warning! For continued protection against risk of fire, use
the correct fuse. Disconnect power cord from mains supply
before replacing fuses.
Avertissement! Pour la protection continue contre le risque
du feu, remplacez le fusible seulement par une du type
spécifique et des estimations courantes. Démontez
l’équipement de l’alimentation d’énergie avant de
remplacer le fusible.
12 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
3.2 Parts and Controls
The following labeled diagrams illustrate the terms used in this manual to describe parts
and controls of the microplate reader. More details on the operation of each feature are
provided in Section 4-Operating Procedures.
3.2.1 Parts of the Instrument
Figure 3.2.1-1 Front of Instrument
Figure 3.2.1-2 Door open, tray out
A) Door
B) Indicator
light
B
A
A) Tray out
(extended)
B) Door open
A
B
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 13
Figure 3.2.1-3 Door open, tray extended loaded with a microplate
Figure 3.2.1-4 Back of Instrument
A) Tray
extended,
loaded with a
microplate
B) Door open
A
B
D) External
Protective
Grounding
Terminal
E) Power Cord
Connection
A
E
C
D
B
A) Power switch
B) Lamp access
door
C) Universal
Serial Bus
(USB)
connection
14 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
3.3 Instrument Setup & Software Installation
IMPORTANT NOTE:
AS WITH MANY USB DEVICES IT IS CRITICAL THAT YOU DO NOT CONNECT THE
USB CABLE TO THE CHROMATE OR COMPUTER UNTIL THE SOFTWARE HAS
BEEN INSTALLED. DOING SO MAY AFFECT THE INSTALLATION OF THE
APPLICABLE DRIVERS NEEDED TO RUN CHROMATE MANAGER.
Follow the steps in Section 3.3.1 Software Installation and then reference Section 3.3.2
for USB port connectivity.
3.3.1 Software Installation
Turn on the computer and insert the USB Mass Storage Device into the USB port of
the computer. Double-click on the setup.exe to begin Setup installation. Follow the
prompts for installing the program and the communications port drivers.
USB Mass Storage Device containing the ChroMate setup.exe (reference Software
Installation)
Steps Prompts/Screen Display
Double-click on
setup.exe to begin
Setup
Figure 3.3.1-1 Double-Click to Begin Setup
ChroMate™ ChroMate™ Operator’s Manual Doc. 4300 Rev. E 15
Steps Prompts/Screen Display
Setup process begins:
Figure 3.3.1-2 Welcome to ChroMate Setup Figure 3.3.1-3 Destination Folder
Figure 3.3.1-4 Program Folder Figure 3.3.1-5 Installation Progress Indicator
Click Finish to complete Setup.
ChroMate Manager Icon
16 ChroMate™ Operator’s Manual Doc. 4300 Rev. E
Steps Prompts/Screen Display
Install the virtual com port (VCP) drivers from the file located in the folder C:\Chromate\USB
drivers (or “[Installation Folder]\USB drivers if you changed the install directory name). From
the USB Drivers folder click on the appropriate executable (.exe) file for the operating system
on your computer (i.e., XP, Vista, Windows98):
The following is an example of the screens that you will encounter during the virtual com port
installation process. Note that the drivers for an XP operating system were chosen for this
example.
Click the Next button:
VCP Driver Welcome Window License Agreement
Destination folder Click the Install button to begin the install process
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Chromate 4300 User manual

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