• SuperFrost® Plus GOLD slides are inherently tissue binding so there is no
need to add spray or adhesives to the slide.
• Allow SuperFrost® Plus GOLD slide to contact the frozen tissue sections
directly in the cryostat using your standard technique.
• Place the slide horizontally on a table top (tissue side up) at room tempera-
ture for one minute. This allows time for the revolutionary surface binding
chemistry in the SuperFrost® Plus GOLD slide to unite with the tissue section.
This short time at room temperature does not cause frozen sections artifact
because the SuperFrost® Plus GOLD slide holds both nuclear and cytoplas-
mic tissue components firmly immobilized in their place during the bonding
process. The longer you allow for bonding time, the more the frozen tissue will
adhere to the slide.
• Postfix the SuperFrost® Plus GOLD slides if you wish, and stain as per your
standard protocol.
• Store the SuperFrost® Plus GOLD slides at room temperature.
thermoscientifi c.com
Slides & Specialty Glass
Tel. +49 (0) 531 59 00 80
Fax +49 (0) 531 50 97 99
menzel.marketing@thermofi sher.com
© 2015 Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of
Thermo Fisher Scientifi c Inc. and its subsidiaries.
Thermo Fisher Scientifi c
Gerhard Menzel B.V. & Co. KG
Saarbrückener Straße 248
38116 Braunschweig, Germany
SuperFrost® Plus GOLD
Instructions For Use
thermoscientifi c.com
Slides & Specialty Glass
Tel. +49 (0) 531 59 00 80
Fax +49 (0) 531 50 97 99
menzel.marketing@thermofi sher.com
© 2015 Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of
Thermo Fisher Scientifi c Inc. and its subsidiaries.
Thermo Fisher Scientifi c
Gerhard Menzel B.V. & Co. KG
Saarbrückener Straße 248
38116 Braunschweig, Germany
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