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Thermo Fisher Scientific Brilliance ESBL User guide

  • Hello! I've reviewed the Thermo Scientific Brilliance ESBL User Guide. This document provides instructions on how to use the Brilliance ESBL Agar for presumptive identification of ESBL-producing organisms. The guide details the color-based results for positive and negative identifications, as well as the incubation process. I'm here to help answer any questions you have about the document or the product.
  • What is the incubation temperature?
    How long should I incubate the plate?
    What does a brown halo indicate?
    What should you do if a plate is negative after 24 hours?
Thermo ScientificTM BrillianceTM ESBL
User Guide
Inoculate plate. Incubate at 35°C +/- 2°C for 24 hrs.
Read results.
presumptive identification of Extended Spectrum ß-Lactamase (ESBL)-producing organisms
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Blue or pink
E. coli
Brown halo
Proteus, Morganella,
Providencia
Green
Klebsiella, Enterobacter,
Serratia and Citrobacter
Colorless
Salmonella, Acinetobacter
or other
Positive
Identifications are presumptive and should be confirmed. Please note, organisms with an atypical enzyme pattern may give anomalous
reactions on Thermo ScientificTM BrillianceTM ESBL Agar. Bacteria with other resistance mechanisms may also produce colonies.
Any such isolate may be clinically significant and should be investigated further.
Negative
Negative plates
should be
re-incubated for an
additional 24 hrs.
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