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Thermo Fisher Scientific Double and Multiple Digestion of DNA User guide

  • Hello! I'm a chat assistant that has reviewed the user guide for FastDigest enzymes. This document outlines the protocol for double and multiple DNA digestion, detailing how to use multiple enzymes, scale reactions, and perform sequential cleavage if needed. I am ready to answer your questions about it.
  • How much enzyme should be used?
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Protocol
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FastDigest® enzymes allow simultaneous digestion of DNA with two or more enzymes in one digestion reaction.
• Use 1 µl of each enzyme and scale up the reaction conditions appropriately.
• The combined volume of all added enzymes should not exceed of the total reaction volume.
• If enzymes require different incubation temperature, perform sequential DNA cleavage: complete the rst digestion
reaction at the lower temperature, add the second enzyme and increase the digestion temperature for the second
enzyme cleavage.
Double and Multiple Digestion of DNA
This protocol is for the
Double and Multiple Digestion of DNA
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