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  3. T3 Promoter Sequencing Primer, 24-mer

Thermo Fisher Scientific T3 Promoter Sequencing Primer, 24-mer User guide

  • I have reviewed the product details for the T3 promoter sequencing primer. It is a single-stranded oligonucleotide designed for sequencing plasmids containing the T3 RNA polymerase promoter sequence. The document provides information on its concentration, volume, and storage conditions. I'm ready to answer your questions about it.
  • What is the primer made of?
    What is the purpose of this primer?
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PRODUCT INFORMATION
T3 promoter sequencing primer,
24-mer
5’-d(GCGCGAAATTAACCCTCACTAAAG)-3’
#SO120 0.1A
260
units
(supplied as 10 pmol/µL aqueous solution)
Lot: _
Concentration: 10 µM (10 pmol/µL)
Volume: 42 µL
T
m
: 70°C
CG content: 46%
Store at -20°C
www.thermoscientific.com/onebio
Description
This primer is single-stranded oligonucleotide that has
5'- and 3'-hydroxyl ends.
The primer can be used to sequence plasmids containing
T3 RNA polymerase promoter sequence. The primer may
be unsuitable for sequencing certain plasmids that
contain altered, although functionally active promoters.
CERTIFICATE OF ANALYSIS
Each lot of T3 promoter sequencing primer is assayed in
the dideoxy sequencing reaction using a vector
containing the T3 promoter sequence.
Quality authorized by: Jurgita Zilinskiene
PRODUCT USE LIMITATION
This product is developed, designed and sold exclusively for research purposes and
in vitro use only. The product was not tested for use in diagnostics or for drug
development, nor is it suitable for administration to humans or animals.
Please refer to www.thermoscientific.com/onebio for Material Safety Data Sheet of
the product.
© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries.
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