Benchmark Scientific P7700-1 Owner's manual

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InstructionManual

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adivisionof

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1

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Introduction……………………………………………………………...1

Parts&Features………………………………………………………..2

TipCompatibility……………………………………………………….

3

Operation………………………………………………………………….

4

TipsforProperPipetting…………………………………………...5

CleaningandSterilization………………………………….........

5

Specifications…………………………………………………………….6

CheckingAccuracyandPrecision……………………………….

6

Calibration…………………………………………………………………8

Troubleshooting………………………………………………………..

9

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Introduction:

AccurisNextPettePipettesaredesignedforlaboratoryresearchuse,andspecificallyforthe

measurementandtransferofaqueoussolutions.TheNextPettepipettesoffervariablevolume

setting,andareavailableindifferentrangestocovervolumemeasurementsfrom0.1ulto

10ml.

NextPettepipettorsutilizetheprincipleofairdisplacement.Avacuumis

createdbythe

plungermovement,andaprecisevolumeofsampleliquidisdrawnintotheattachedpipette

tip.Disposablepipettipsmustalwaysbeusedforliquidaspiration,andtheshaftsofthe

NextPettepipettesaredesignedtoacceptawiderangeofdifferentbrandsandstylesof

disposable

pipettetips.

Properoperationandproperuseofdisposabletipswillpreventliquidsamplesfromentering

thepipetteandcontaminatingtheinternalparts.

EachvariablevolumeNextPetteincorporatesa4‐digitcounterdisplaytoshowthesetvolume.

Thevolumesettingcanbechangedusingthevolumeadjustmentdialbelowthepushbutton.A

volumeadjustmentlockislocatedjustbelowthetipejectorbutton,andthisallowstheuserto

securelylocktheadjustmentdialattheappropriatelysetvolume.

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2

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Thetopofthefingerhookhasaplaceundertheremovablewindowfor

anidentificationlabel.Eachpipettecomeswithasetofdifferent

coloredlabelsforcolorcoding.Underthewindowandlabelistheserial

numberlabelandcalibrationswitch.Seethecalibrationsectionofthis

manualfor

details.

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3

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TheNextPettePipettesfeatureauniversaltipfittingshaftthatisdesignedtoworkwithmost

manufacturer’sbrandsofpipettetips.Beforepipetting,alwaysensurethatapipettetipofthe

appropriatesizehasbeenattached.

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SeebelowforachartidentifyingtheappropriatetipsizeforeachNextPettePipette:

P77700‐1:   0.1‐1μl   10ultip

P7700‐10  0.5‐10μl   10ultip

P7700‐20  2‐20μl   200ultip

P7700‐100  10‐100μl  200ultip

P7700‐200  20‐200μl  200ultip

P7700‐1000  100‐1000μl  1000ultip

P7700‐5M  500‐5000μl  5000ultip

P7700‐10M  1000‐10000μl  10,000ultip

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AttachingtheTip:

Attachthetipbyfirmlypressingthepipetteconeintothetip.Apropersealisnecessaryfor

optimalpipettingaccuracyandprecision.



NOTE:For5mland10mlpipettes(P7700‐5MandP7700‐10M)alwaysensurethattheincluded

filterisproperlyinstalledontothepipetteshaftpriortotipattachment.Failuretodosocan

resultinharmtotheinternalcomponentsofthepipette.

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4

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(Beforeoperation,pleaserefertothe“attachingthetip”instructionsabove.)

1. Volumesetting:

Ensurethatthevolumeadjustmentlockisintheunlockedposition

(pushedup).Turnthevolumeadjustmentdialandmonitorthe



volumedisplaytoselectthepipettingvolume.Whenthevolume

isselected,pushthevolumelocktothelockedposition.

2. Aspiratetheliquidsample:

Pushtheplungerbuttondownwiththethumbtothefirststop.

Holdingthepipettevertically,immersethepipettipintotheliquidsample2mmto3mm.

Slowlyreleasepressureontheplungerbuttonsothattheliquidsampleisdrawnintothe

pipettip.NOTE:Itisveryimportantthatthesampleliquidisaspiratedslowly.Leavethe

tipimmersedinthesamplefor1to2secondsthenremoveitfromthesample.

NOTE:Alwayskeepthepipetteinanuprightverticalpositionwhenthereisliquidinthe

pipettetip.Layingthepipettehorizontallymaycauseliquidtoflowfromthetipinto the 

pipetteshaft,causingcontamination.

3. Dispensing

Placethepipettipsoitistouchingthewallofthecontainerortubeintowhichthesample

willbedispensed.Thepipetteshouldbeheldata30to40degreeanglerelativetothewall

ofthecontainer.

Presstheplungerbuttonslowlytothefirststoptodispensethesample.Increasepressure

ontheplungerbuttontopassthefirststopandreachthesecondstop(thisistheblowout

stepwhichhelpstodispensethefullamountofsamplefromthetip).

Duringtheblowoutstep,slowlymovethepipettipagainstthe wallofthevessel.Thiswill

helptoremoveanyremainingliquidfromthepipettip.

Removethepipettetipsawayfromthevesselwallandallowtheplungertoreturntothe

startingposition.

4. Ejectingthetip

Holdthepipetteoverasuitablewastecontainerandpressthetipejectorbuttontoremove

thetip.



1.2.3.4.



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SecondStop

VolumeSetting

Aspirating

FirstStop

Dispensing Ejectingtip

Example:

5

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Accuratepipettingrequiresskillsthatimprovewithpractice.Itisalsonecessarytofollow

recommendedbestpracticesandunderstandproperpipettingtechnique.Thefollowingare

recommendationsonhowtogetthebestperformancefromyourNextPettepipette:



 Alwaysoperatetheplungerbuttonslowlyandsmoothlywhenaspiratinganddispensing

samples.

 Theendofthepipettetipsshouldbeimmersed2to3mmbelowthesurfaceofthe

sampletobeaspirated,andthisdepthorimmersionshouldbekeptconstantduringthe

aspiration.

 Alwayskeepthepipetteinaverticalpositionduringthepipettingprocess.

 Useanewpipettetipwheneverswitchingfromonetypeofsampletoanother.

 Carefullycheckthepipettetipsused.Anyinconsistenciesinmanufacturewillresultin

inaccurateandimpreciseresults.

 Carefullycheckthepipettetipsforleftoversampleliquidstayinginsidethetipduring

thepipettingprocess.

 Alwaysusethepre‐rinsingtechniqueonanewtipbeforeaspiratingthemeasured

volume.

 Sampleliquidshouldnotenterthepipetteshaft.

 Neverlaythepipettehorizontallywhenthereissampleliquidinapipettetip.

 Donotaspirateliquidsabove70C.

 Afterpipettingacidsoranyotheraggressiveliquids,itisrecommendedtodisassemble

thepipetteandrinsetheplunger,shaft,sealandotherinternalelementswithdistilled

water.



Cleaning:Externalsurfacesofthepipettecanbecleanedwithwaterorisopropylalcohol.

Sterilization:TheNextPetteisfullyautoclavableat121°Cfor20minutes.Afterautoclaving,itis

importanttoletthepipettecompletelycooltoroomtemperaturepriortouse.

Important:Alwayschecktheautoclavetoconfirmthemaximumtemperaturesettingof121°C

priortoautoclavingtheNextPette.Temperatureshigherthan121°Corasterilizationprocess

longerthan20minutesmaydamagetheNextPette.

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6

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Item No. Volume Increment

A*≤±%

Min volume

A*≤±%

Max volume

CV*≤±%

Min volume

CV*≤±%

Max volume

P7700-1

0.1-1μl （0.001ul)

20.00 2.00 12.00 1.20

P7700-10

0.5-10μl （0.01ul)

3.80 0.50 4.00 0.50

P7700-20

2-20μl （0.02ul)

3.00 0.80 3.00 0.80

P7700-100

10-100μl （0.1ul)

1.50 0.80 1.00 0.20

P7700-200

20-200μl （0.2ul)

1.20 0.60 1.50 0.80

P7700-1000

100-1000μl （1ul)

1.50 0.60 1.00 0.60

P7700-5M

500-5000μl （5ul)

0.60 0.50 0.60 0.50

P7700-10M

1000-10000μl （10ul)

2.50 0.50 2.00 0.50



ItisrecommendedthateachNextPettepipetteischeckedonaregularbasistoconfirmthatitis

withinitscalibrationspecifications.



Thegravimetrictestmethod(accordingtoDINENISO8655)isrecommendedforchecking

accuracyandprecision.



Itisrecommendedtouseafullycalibratedelectronicbalanceforthegravimetrictesting.A4

digitbalancecanbeusedtotestvolumesgreaterthan10ul.For10ulandlessa5digitbalance

isrecommended.For1.0ulandless,a6digitbalanceisrecommended.



1. Setthemaximumvolumeonthepipette.

2. Conditionthepipettebeforetesting.Attachanappropriatepipettetip,aspirateand

dispensethetestliquid(distilledwater,at20°C,isrecommended)5times.Discardthe

pipettetip.

3. Performagravimetrictest:

a. Attachanewpipettetip.Note:thepipettetipsshouldbeofhighquality,and

shouldbethesametipsthat

willbeusedonaregularbasiswiththepipette.

b. Pre‐rinsethetipsafewtimesbyaspiratinganddispensingthetestliquid.This

willcoattheinnersurfaceofthetipwithliquidandenableanaccuratetest.

c. Weighthemeasuredvolumeofliquid fromthepipette

byaspiratingtoa

weighingvesselonthebalance.

d. Calculatetheliquidvolumebyconvertingusingthecorrectionfactorconsidering

thetemperatureofthewater.

e. Atleast10aspirationsandweighingsin3volumeranges:100%,50%,10%of

nominalvolume,arerecommendedinordertocalculatethe

accuracyand

precisionresults

7



Calculation(fornominalvolume)

=Weighingresults Z=Correctionfactor

N=Numberofweighings (e.g.1.0029ul/mgat20°C,1013hPa)



  



Accuracy CoefficientofVariation



    





StandardDeviation



NOTE:TheaccuracyandprecisionspecificationshavebeendevelopedusingBenchmark

pipettetips,usingthegravimetricmethodandperforming10measurementsofdistilled

wateratatemperatureof20°C+/‐1°CaccordingtotheENISO8655standard.



8



TheNextPetteisfactorycalibratedforusewithaqueoussolutions.Ifthepipetteoperationis

inaccuratewhenpipettingaqueoussolutions,oritisdesiredtopipetteliquidsofadensityor

viscositydifferentthanwater,orspecialtypesofpipettetipsaretobeused,itmaybe

necessarytoadjust

thecalibration.



1. Settheadjustmentdialofthepipettortoavolumefortesting(ie.Thenominalvolume

ofthepipette).

2. Pipetteameasuredvolumeofsampleliquidontoananalyticalbalance,andusingthe

gravimetricmethod(withtheappropriatecorrectionfactor),calculatetheactual

volumeofthepipettedsample.Iftheactualvolumediffersfromthevolumesetonthe

NextPettedisplay,itisnecessarytoadjustthecalibration.

3. Removetheclearwindowandlabelonthetopsurfaceofthefingerhook.

4. Pushtheredcalibrationlockawayfromtheadjustmentdialandsimultaneouslypull

upwardonthevolumesettingdialuntilitclicksintotheraisedposition.Releasethered

calibrationlock.

5. Turnthevolumeadjustmentdialtothevolumethathasbeencalculatedforthesample

thatwasmeasured(theactualvolume).

6. Pushthecalibrationlockagainawayfromtheadjustmentdial,simultaneouslypushthe

volumesettingdialdownuntilitlocksintoposition.Releasethecalibrationlock.

7. AdjustthevolumeoftheNextPettebacktothenominalvolume,andtestthecalibration

againusingthegravimetricmethod.Ifthevalueisstilloff,repeatthecalibration

procedure.



3.Removethe

clearwindow

4.Pullupvolume

adjustmentdial

5.Turnvolume

adjustmentdial

6.Pushdown

volumesettingdial

9



Dropletsofliquidremaininthetipafterdispensing:

Thetipisemptiedtoofast

 Solution1.)Decreasethespeedandforcewhenpressingtheplungerbutton



Thetiphasbeenoverused

 Solution1.)Replacethepipettetipwithanewone



Airpocketsappearintheliquid

ofthepipettetip

Thesampleisaspiratedtoofast

 Solution1.)Decreasethespeedofaspiration(raisethepipettingbuttonsmoothly

duringsampleuptake)

Thepipettetipisdefective

 Solution2.)Checkthepipettetipforqualityissues,replacethepipettetip



Plungerbuttonisdifficulttodepress

(ordoesnotmovesmoothly):

Thepistonmaybecontaminatedoroutofshape.

Solution1.)Cleanthepistonandapplysiliconelubricant

Solution2.)Replacethepistonorlowerportionofthepipette



Thetipisleakingordrippingliquid:

Thepipettetipisdefectiveornotcompatible

 Solution

1.)Replacethepipettetipwithanewone

 Solution2.)Replacethepipettetipwithanewonefromadifferentbrand/style



Thetipisnotproperlysealedonthepipetteshaft

 Solution1.)Pressthetipfirmlyonthepipetteshaft



Theinternalseal,O‐ring,pistonorpipetteshaftiscontaminatedofdamaged

 Solution1.)Disassembleandcleanthepistonandsealwithdistilledwater

 Solution2.)Checkthepartsfordamage/wear.Replacepartasrequired



Thedispensedvolumeismoreorlessthanthesetvolume:

Plunger

buttonhasbeenpressedtoofar(pasttheblowoutphase)beforesampleuptake

 Solution1.)Operatecarefully,usingproperpipettingtechnique



Pipetteisoutofcalibration

 Solution1.)Recalibratethepipettefollowingtheinstructionsinthemanual

10





11



adivisionof



Benchmark Scientific P7700-1, P7700-10, P7700-100, P7700-1000, P7700-10M, P7700-20, P7700-200, P7700-5M, P7700-CAR, P7700-T10M, P7700-T5M Owner's manual

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