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Dropletsofliquidremaininthetipafterdispensing:
Thetipisemptiedtoofast
Solution1.)Decreasethespeedandforcewhenpressingtheplungerbutton
Thetiphasbeenoverused
Solution1.)Replacethepipettetipwithanewone
Airpocketsappearintheliquid
ofthepipettetip
Thesampleisaspiratedtoofast
Solution1.)Decreasethespeedofaspiration(raisethepipettingbuttonsmoothly
duringsampleuptake)
Thepipettetipisdefective
Solution2.)Checkthepipettetipforqualityissues,replacethepipettetip
Plungerbuttonisdifficulttodepress
(ordoesnotmovesmoothly):
Thepistonmaybecontaminatedoroutofshape.
Solution1.)Cleanthepistonandapplysiliconelubricant
Solution2.)Replacethepistonorlowerportionofthepipette
Thetipisleakingordrippingliquid:
Thepipettetipisdefectiveornotcompatible
Solution
1.)Replacethepipettetipwithanewone
Solution2.)Replacethepipettetipwithanewonefromadifferentbrand/style
Thetipisnotproperlysealedonthepipetteshaft
Solution1.)Pressthetipfirmlyonthepipetteshaft
Theinternalseal,O‐ring,pistonorpipetteshaftiscontaminatedofdamaged
Solution1.)Disassembleandcleanthepistonandsealwithdistilledwater
Solution2.)Checkthepartsfordamage/wear.Replacepartasrequired
Thedispensedvolumeismoreorlessthanthesetvolume:
Plunger
buttonhasbeenpressedtoofar(pasttheblowoutphase)beforesampleuptake
Solution1.)Operatecarefully,usingproperpipettingtechnique
Pipetteisoutofcalibration
Solution1.)Recalibratethepipettefollowingtheinstructionsinthemanual