Benchmark Scientific P7700-5M Owner's manual

Category
Measuring, testing & control
Type
Owner's manual
InstructionManual
adivisionof
1
Introduction……………………………………………………………...1
Parts&Features………………………………………………………..2
TipCompatibility……………………………………………………….
3
Operation………………………………………………………………….
4
TipsforProperPipetting…………………………………………...5
CleaningandSterilization………………………………….........
5
Specifications…………………………………………………………….6
CheckingAccuracyandPrecision……………………………….
6
Calibration…………………………………………………………………8
Troubleshooting………………………………………………………..
9
Introduction:
AccurisNextPettePipettesaredesignedforlaboratoryresearchuse,andspecificallyforthe
measurementandtransferofaqueoussolutions.TheNextPettepipettesoffervariablevolume
setting,andareavailableindifferentrangestocovervolumemeasurementsfrom0.1ulto
10ml.
NextPettepipettorsutilizetheprincipleofairdisplacement.Avacuumis
createdbythe
plungermovement,andaprecisevolumeofsampleliquidisdrawnintotheattachedpipette
tip.Disposablepipettipsmustalwaysbeusedforliquidaspiration,andtheshaftsofthe
NextPettepipettesaredesignedtoacceptawiderangeofdifferentbrandsandstylesof
disposable
pipettetips.
Properoperationandproperuseofdisposabletipswillpreventliquidsamplesfromentering
thepipetteandcontaminatingtheinternalparts.
EachvariablevolumeNextPetteincorporatesa4digitcounterdisplaytoshowthesetvolume.
Thevolumesettingcanbechangedusingthevolumeadjustmentdialbelowthepushbutton.A
volumeadjustmentlockislocatedjustbelowthetipejectorbutton,andthisallowstheuserto
securelylocktheadjustmentdialattheappropriatelysetvolume.
2
Thetopofthefingerhookhasaplaceundertheremovablewindowfor
anidentificationlabel.Eachpipettecomeswithasetofdifferent
coloredlabelsforcolorcoding.Underthewindowandlabelistheserial
numberlabelandcalibrationswitch.Seethecalibrationsectionofthis
manualfor
details.
3
TheNextPettePipettesfeatureauniversaltipfittingshaftthatisdesignedtoworkwithmost
manufacturer’sbrandsofpipettetips.Beforepipetting,alwaysensurethatapipettetipofthe
appropriatesizehasbeenattached.
SeebelowforachartidentifyingtheappropriatetipsizeforeachNextPettePipette:
P777001: 0.11μl 10ultip
P770010 0.510μl 10ultip
P770020 220μl 200ultip
P7700100 10100μl 200ultip
P7700200 20200μl 200ultip
P77001000 1001000μl 1000ultip
P77005M 5005000μl 5000ultip
P770010M 100010000μl 10,000ultip
AttachingtheTip:
Attachthetipbyfirmlypressingthepipetteconeintothetip.Apropersealisnecessaryfor
optimalpipettingaccuracyandprecision.
NOTE:For5mland10mlpipettes(P77005MandP770010M)alwaysensurethattheincluded
filterisproperlyinstalledontothepipetteshaftpriortotipattachment.Failuretodosocan
resultinharmtotheinternalcomponentsofthepipette.
4
(Beforeoperation,pleaserefertothe“attachingthetip”instructionsabove.)
1. Volumesetting:
Ensurethatthevolumeadjustmentlockisintheunlockedposition
(pushedup).Turnthevolumeadjustmentdialandmonitorthe
volumedisplaytoselectthepipettingvolume.Whenthevolume
isselected,pushthevolumelocktothelockedposition.
2. Aspiratetheliquidsample:
Pushtheplungerbuttondownwiththethumbtothefirststop.
Holdingthepipettevertically,immersethepipettipintotheliquidsample2mmto3mm.
Slowlyreleasepressureontheplungerbuttonsothattheliquidsampleisdrawnintothe
pipettip.NOTE:Itisveryimportantthatthesampleliquidisaspiratedslowly.Leavethe
tipimmersedinthesamplefor1to2secondsthenremoveitfromthesample.
NOTE:Alwayskeepthepipetteinanuprightverticalpositionwhenthereisliquidinthe
pipettetip.Layingthepipettehorizontallymaycauseliquidtoflowfromthetipinto the
pipetteshaft,causingcontamination.
3. Dispensing
Placethepipettipsoitistouchingthewallofthecontainerortubeintowhichthesample
willbedispensed.Thepipetteshouldbeheldata30to40degreeanglerelativetothewall
ofthecontainer.
Presstheplungerbuttonslowlytothefirststoptodispensethesample.Increasepressure
ontheplungerbuttontopassthefirststopandreachthesecondstop(thisistheblowout
stepwhichhelpstodispensethefullamountofsamplefromthetip).
Duringtheblowoutstep,slowlymovethepipettipagainstthe wallofthevessel.Thiswill
helptoremoveanyremainingliquidfromthepipettip.
Removethepipettetipsawayfromthevesselwallandallowtheplungertoreturntothe
startingposition.
4. Ejectingthetip
Holdthepipetteoverasuitablewastecontainerandpressthetipejectorbuttontoremove
thetip.
1.2.3.4.

SecondStop
VolumeSetting
Aspirating
FirstStop
Dispensing Ejectingtip
Example:
5
Accuratepipettingrequiresskillsthatimprovewithpractice.Itisalsonecessarytofollow
recommendedbestpracticesandunderstandproperpipettingtechnique.Thefollowingare
recommendationsonhowtogetthebestperformancefromyourNextPettepipette:
Alwaysoperatetheplungerbuttonslowlyandsmoothlywhenaspiratinganddispensing
samples.
Theendofthepipettetipsshouldbeimmersed2to3mmbelowthesurfaceofthe
sampletobeaspirated,andthisdepthorimmersionshouldbekeptconstantduringthe
aspiration.
Alwayskeepthepipetteinaverticalpositionduringthepipettingprocess.
Useanewpipettetipwheneverswitchingfromonetypeofsampletoanother.
Carefullycheckthepipettetipsused.Anyinconsistenciesinmanufacturewillresultin
inaccurateandimpreciseresults.
Carefullycheckthepipettetipsforleftoversampleliquidstayinginsidethetipduring
thepipettingprocess.
Alwaysusetheprerinsingtechniqueonanewtipbeforeaspiratingthemeasured
volume.
Sampleliquidshouldnotenterthepipetteshaft.
Neverlaythepipettehorizontallywhenthereissampleliquidinapipettetip.
Donotaspirateliquidsabove70C.
Afterpipettingacidsoranyotheraggressiveliquids,itisrecommendedtodisassemble
thepipetteandrinsetheplunger,shaft,sealandotherinternalelementswithdistilled
water.
Cleaning:Externalsurfacesofthepipettecanbecleanedwithwaterorisopropylalcohol.
Sterilization:TheNextPetteisfullyautoclavableat121°Cfor20minutes.Afterautoclaving,itis
importanttoletthepipettecompletelycooltoroomtemperaturepriortouse.
Important:Alwayschecktheautoclavetoconfirmthemaximumtemperaturesettingof121°C
priortoautoclavingtheNextPette.Temperatureshigherthan121°Corasterilizationprocess
longerthan20minutesmaydamagetheNextPette.
6
Item No. Volume Increment
A*≤±%
Min volume
A*±%
Max volume
CV*≤±%
Min volume
CV*≤±%
Max volume
P7700-1
0.1-1μl 0.001ul)
20.00 2.00 12.00 1.20
P7700-10
0.5-10μl 0.01ul)
3.80 0.50 4.00 0.50
P7700-20
2-20μl 0.02ul)
3.00 0.80 3.00 0.80
P7700-100
10-100μl 0.1ul)
1.50 0.80 1.00 0.20
P7700-200
20-200μl 0.2ul)
1.20 0.60 1.50 0.80
P7700-1000
100-1000μl 1ul)
1.50 0.60 1.00 0.60
P7700-5M
500-5000μl 5ul)
0.60 0.50 0.60 0.50
P7700-10M
1000-10000μl 10ul)
2.50 0.50 2.00 0.50
ItisrecommendedthateachNextPettepipetteischeckedonaregularbasistoconfirmthatitis
withinitscalibrationspecifications.
Thegravimetrictestmethod(accordingtoDINENISO8655)isrecommendedforchecking
accuracyandprecision.
Itisrecommendedtouseafullycalibratedelectronicbalanceforthegravimetrictesting.A4
digitbalancecanbeusedtotestvolumesgreaterthan10ul.For10ulandlessa5digitbalance
isrecommended.For1.0ulandless,a6digitbalanceisrecommended.
1. Setthemaximumvolumeonthepipette.
2. Conditionthepipettebeforetesting.Attachanappropriatepipettetip,aspirateand
dispensethetestliquid(distilledwater,at20°C,isrecommended)5times.Discardthe
pipettetip.
3. Performagravimetrictest:
a. Attachanewpipettetip.Note:thepipettetipsshouldbeofhighquality,and
shouldbethesametipsthat
willbeusedonaregularbasiswiththepipette.
b. Prerinsethetipsafewtimesbyaspiratinganddispensingthetestliquid.This
willcoattheinnersurfaceofthetipwithliquidandenableanaccuratetest.
c. Weighthemeasuredvolumeofliquid fromthepipette
byaspiratingtoa
weighingvesselonthebalance.
d. Calculatetheliquidvolumebyconvertingusingthecorrectionfactorconsidering
thetemperatureofthewater.
e. Atleast10aspirationsandweighingsin3volumeranges:100%,50%,10%of
nominalvolume,arerecommendedinordertocalculatethe
accuracyand
precisionresults
7
Calculation(fornominalvolume)
X
1
=Weighingresults Z=Correctionfactor
N=Numberofweighings (e.g.1.0029ul/mgat20°C,1013hPa)
 
Accuracy CoefficientofVariation


StandardDeviation
NOTE:TheaccuracyandprecisionspecificationshavebeendevelopedusingBenchmark
pipettetips,usingthegravimetricmethodandperforming10measurementsofdistilled
wateratatemperatureof20°C+/‐1°CaccordingtotheENISO8655standard.
8
TheNextPetteisfactorycalibratedforusewithaqueoussolutions.Ifthepipetteoperationis
inaccuratewhenpipettingaqueoussolutions,oritisdesiredtopipetteliquidsofadensityor
viscositydifferentthanwater,orspecialtypesofpipettetipsaretobeused,itmaybe
necessarytoadjust
thecalibration.
1. Settheadjustmentdialofthepipettortoavolumefortesting(ie.Thenominalvolume
ofthepipette).
2. Pipetteameasuredvolumeofsampleliquidontoananalyticalbalance,andusingthe
gravimetricmethod(withtheappropriatecorrectionfactor),calculatetheactual
volumeofthepipettedsample.Iftheactualvolumediffersfromthevolumesetonthe
NextPettedisplay,itisnecessarytoadjustthecalibration.
3. Removetheclearwindowandlabelonthetopsurfaceofthefingerhook.
4. Pushtheredcalibrationlockawayfromtheadjustmentdialandsimultaneouslypull
upwardonthevolumesettingdialuntilitclicksintotheraisedposition.Releasethered
calibrationlock.
5. Turnthevolumeadjustmentdialtothevolumethathasbeencalculatedforthesample
thatwasmeasured(theactualvolume).
6. Pushthecalibrationlockagainawayfromtheadjustmentdial,simultaneouslypushthe
volumesettingdialdownuntilitlocksintoposition.Releasethecalibrationlock.
7. AdjustthevolumeoftheNextPettebacktothenominalvolume,andtestthecalibration
againusingthegravimetricmethod.Ifthevalueisstilloff,repeatthecalibration
procedure.
3.Removethe
clearwindow
4.Pullupvolume
adjustmentdial
5.Turnvolume
adjustmentdial
6.Pushdown
volumesettingdial
9
Dropletsofliquidremaininthetipafterdispensing:
Thetipisemptiedtoofast
Solution1.)Decreasethespeedandforcewhenpressingtheplungerbutton
Thetiphasbeenoverused
Solution1.)Replacethepipettetipwithanewone
Airpocketsappearintheliquid
ofthepipettetip
Thesampleisaspiratedtoofast
Solution1.)Decreasethespeedofaspiration(raisethepipettingbuttonsmoothly
duringsampleuptake)
Thepipettetipisdefective
Solution2.)Checkthepipettetipforqualityissues,replacethepipettetip
Plungerbuttonisdifficulttodepress
(ordoesnotmovesmoothly):
Thepistonmaybecontaminatedoroutofshape.
Solution1.)Cleanthepistonandapplysiliconelubricant
Solution2.)Replacethepistonorlowerportionofthepipette
Thetipisleakingordrippingliquid:
Thepipettetipisdefectiveornotcompatible
Solution
1.)Replacethepipettetipwithanewone
Solution2.)Replacethepipettetipwithanewonefromadifferentbrand/style
Thetipisnotproperlysealedonthepipetteshaft
Solution1.)Pressthetipfirmlyonthepipetteshaft
Theinternalseal,Oring,pistonorpipetteshaftiscontaminatedofdamaged
Solution1.)Disassembleandcleanthepistonandsealwithdistilledwater
Solution2.)Checkthepartsfordamage/wear.Replacepartasrequired
Thedispensedvolumeismoreorlessthanthesetvolume:
Plunger
buttonhasbeenpressedtoofar(pasttheblowoutphase)beforesampleuptake
Solution1.)Operatecarefully,usingproperpipettingtechnique
Pipetteisoutofcalibration
Solution1.)Recalibratethepipettefollowingtheinstructionsinthemanual
10

11
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Benchmark Scientific P7700-5M Owner's manual

Category
Measuring, testing & control
Type
Owner's manual

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