Thermo Fisher Scientific KingFisher Duo Prime Owner's manual

Thermo Scientific

KingFisher Duo Prime

Technical Manual

Rev. 1.0, Cat. no. N16621

KingFisher Duo Prime magnetic particle processor and BindIt Software are registered trademarks of Thermo

Fisher Scientific Inc.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-

free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that

might result from any use of this document, even if the information in the document is followed

properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history:

March 2015: First release for KingFisher Duo Prime

For Research Use Only. Not for use in diagnostic procedures.

WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic Equip-

ment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each

European Union (EU) Member State, and these companies should dispose of or recycle this product.

Contact Thermo for further information on Thermo Fisher Scientific's compliance

with these Directives and the recyclers in your country.

Thermo Fisher Scientific Thermo Scientific KingFisher Duo PrimeTechnical Manual iii

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Preface

About This User Manual

This user manual is written for the actual end user, for example, research scientist or laboratory

technician. This manual provides information on the Thermo Scientific™ KingFisher™ Duo Prime

magnetic particle processor, including the installation and operating instructions.

This document aims to give you the information you need for:

• Reviewing safety precautions

• Installing the KingFisher Duo Prime

• Using the KingFisher Duo Prime in routine jobs – the processing step

• Performing basic cleaning and maintenance procedures

• Troubleshooting the instrument performance

This user manual also describes the features and specifications of the KingFisher Duo Prime

instrument as well as ordering information.

Note that simulated data appears in the screen captures.

Read the manual in its entirety before operating the instrument.

Keep the user manual for future reference. The user manual is an important part of the instrument and

should be readily available during the use of the instrument.

Related Documentation

In addition to this guide, Thermo Fisher Scientific provides the following documents for KingFisher

Duo Prime:

• Thermo Scientific KingFisher Duo Prime Brief User Manual (Cat.no. N16622)

• For PC software related issues, see Thermo Fisher Scientific™ BindIt™ Software User Manual (Cat.

no. N07974)

For the latest information on products and services, visit our websites at

http://www.thermoscientific.com/kingfisher

Preface

Safety and Special Notices

iv Thermo Scientific KingFisher Duo PrimeTechnical Manual Thermo Fisher Scientific

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and other

special notices appear in boxes.

Safety and special notices include the following:

WARNING Risk of electric shock.

WARNING Biohazard risk.

WARNING Risk of injury to the user or users.

WARNING Strong magnetic fields.

CAUTION Risk of damage to the instrument, other equipment or loss of performance or function

in a specific application.

Note Marks a hint, important information that is useful for the optimum operation of the system,

or an item of interest.

Tip Highlights helpful information that can make a task easier.

Preface

Safety and Special Notices

Thermo Fisher Scientific Thermo Scientific KingFisher Duo PrimeTechnical Manual v

Safety Symbols and Markings on KingFisher Duo Prime

The following symbols and markings appear on the type label and the instrument itself:

Instrument Safety and Guidelines for Use

Take into account the following instrument safety considerations and guidelines for correct use:

• Before operating the instrument, read this user manual in its entirety. Failure to read, understand,

and follow the instructions in the manual may result in damage to the instrument, injury to

laboratory and operating personnel, or poor instrument performance.

• Always follow basic safety precautions and local safety guidelines when using the KingFisher Duo

Prime to reduce the risk of injury, biohazardous contamination, fire, or electric shock.

• Before operating the instrument, read this user manual in its entirety. Failure to read, understand,

and follow the instructions in the manual may result in damage to the instrument, injury to

laboratory and operating personnel, or poor instrument performance.

• Observe all “Warning”, “Caution”, and “Note” statements as well as safety symbols and markings

on the instrument and in the documentation.

• The device is allowed to be operated only with software specifically designed for the device.

WARNING UV light

WARNING This product contains very strong permanent magnets.

WARNING Risk of burns. The heating block and/or the elution block surface can be hot. The

warning lights are lit if the temperature rises too high.

WARNING Risk of injury because of moving parts.

Preface

Contacting Us

vi Thermo Scientific KingFisher Duo PrimeTechnical Manual Thermo Fisher Scientific

• Never open any other covers of the KingFisher Duo Prime than the front lid or the maintenance

door (see Figure 2 on page 3) while the instrument is plugged into a power source.

• Never force a microplate onto the instrument.

• The KingFisher Duo Prime is intended for laboratory research use only. Observe proper laboratory

safety precautions, such as wearing protective clothing and following approved laboratory safety

procedures.

• Preventative maintenance instructions should be followed closely to keep the instrument in the

best condition for maximum reliability. A poorly maintained instrument does not give the best

results.

Contacting Us

For the latest information on products and services, visit our website at:

www.thermoscientific.com/kingfisher

Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual vii

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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii

About This User Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii

Safety and Special Notices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .iv

Safety Symbols and Markings on KingFisher Duo Prime. . . . . . . . . . . . . . . . . v

Instrument Safety and Guidelines for Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Contacting Us. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi

Chapter 1 Introduction to KingFisher Duo Prime. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Operation Principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Chapter 2 Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Instrument Layout. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Back and Internal View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

King Fisher Duo Prime Magnetic Particle Processor . . . . . . . . . . . . . . . . . . . . . . 6

Principle of Magnetic Particle Processing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Working with Magnetic Rod . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Collecting Magnetic Particles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Releasing Magnetic Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Washing Magnetic Particles and Incubation . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Changing Volume During Magnetic Particle Processing . . . . . . . . . . . . . . . . . 9

Magnet Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Heating Blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Elution Blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Shield Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

USB Port for PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

USB Ports for Memory Devices and Barcode Reader. . . . . . . . . . . . . . . . . . . . . 13

Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Chapter 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Checking Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Unpacking Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Removing Internal Cushions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Operating Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Contents

viii Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific

Precautions and Limitations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Installing KingFisher Duo Prime . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Releasing Transport Lock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Connecting Power Supply Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Installing Heating Blocks and Magnets Heads . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Operational Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Chapter 4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Using KingFisher Duo Prime PC Software . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Using Internal Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Viewing and Defining Instrument Options and Settings . . . . . . . . . . . . . . . . . . 39

Switching UV Lamp On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Exporting Device Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Exporting Run Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Importing Protocols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Exporting Protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Selecting Maintenance Protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Viewing Instrument Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Setting Internal Software Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Setting Buzzer On or Off. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Defining Date and Time Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Handling Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Tip Combs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Elution Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Starting KingFisher Duo Prime . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Stopping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Emergency Situations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Regular and Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Cleaning Turntable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Cleaning Magnetic Rods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Cleaning Shield Plate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Disposal of Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Decontamination Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Packing Instrument for Transportation or Service . . . . . . . . . . . . . . . . . . . . . . . 62

Refitting Transport Lock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Placing Internal Cushions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Service Contracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Maintaining System Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Disposal of Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Chapter 6 Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

Performance Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Contents

Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual ix

Safety Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Chapter 7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

Chapter 8 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75

KingFisher Duo Prime. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

Accessories and Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

KingFisher Pure Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Chapter 9 Appendix A. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Certificate of Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Chapter 10 Appendix B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

System Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Contents

xThermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific

Thermo Fisher Scientific Thermo Scientific KingFisher Duo PrimeTechnical Manual 1

1

Introduction to KingFisher Duo Prime

Intended Use

The KingFisher Duo Prime magnetic particle processor (see Figure 1) is intended for professional

research use by trained personnel. The instrument is intended for automated transfer and processing of

magnetic particles in a microplate format. Use for self-testing is excluded. It is recommended that

Good Laboratory Practice (GLP) is followed to guarantee reliable analyses.

For technical information on the instrument, see “Technical Specifications” on page 67.

Operation Principle

The patented technology of the KingFisher Duo Prime system is based on the use of magnetic rods

covered with a disposable, specially designed tip comb and plates. The instrument functions without

any dispensing or aspiration parts or devices.

Samples and reagents, including magnetic particles, are dispensed into the plates according to the

corresponding instructions. The protocol that you have selected with the keypad and display has

already been transferred onto the onboard software. You can use the Thermo Scientific™ BindIt™

Software to create and run protocols.

1 Introduction to KingFisher Duo Prime

2Thermo Scientific KingFisher Duo PrimeTechnical Manual Thermo Fisher Scientific

Figure 1. KingFisher Duo Prime magnetic particle processor

To keep the instrument in the best condition for maximum reliability, follow the preventative

maintenance instructions. A poorly maintained instrument does not give the best results. For more

information on maintenance, see chapter

.

Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 3

2

Functional Description

Instrument Layout

This section shows the front, back and internal views of the KingFisher Duo Prime instrument.

Front View

The following figure illustrates the KingFisher Duo Prime instrument and its parts (listed in the table

that follows).

Figure 2. KingFisher Duo Prime front view with front lid open

Item number Description Item number Description

1 Front lid open 4 Elution block (fixed)

2 Maintenance door 5 Interchangeable heating block

3 Interchangeable KingFisher

Duo Prime head

6Turntable

2 Functional Description

Instrument Layout

4Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific

The following figure shows the KingFisher Duo front view with plates and front lid and the

maintenance door closed.

Figure 3. KingFisher Duo Prime front view with plates and front lid closed

Item

number Description Item

number Description

1 Maintenance door 6 Front lid (closed)

2 Shield plate 7 USB port for memory stick or barcode reader.

USB port also at the back of the device.

3 Cooling air outlet 8 ON/OFF switch

4Display 9UV lamp

5 Keypad 10 USB port and barcode reader connection

(preferred location)

2 Functional Description

Instrument Layout

Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 5

Back and Internal View

The back view of the KingFisher Duo Prime instrument is shown in Figure 4 and the internal view in

Figure 5.

Figure 4. KingFisher Duo Prime back view

Item number Description

1 Cooling air inlet

2USB port for PC

3 USB port for memory stick or barcode reader

4 Mains power supply connector

5Transport lock

6Type label

2 Functional Description

King Fisher Duo Prime Magnetic Particle Processor

6Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific

The following figure shows the KingFisher Duo Prime internal view from the front processing

position.

Figure 5. KingFisher Duo Prime internal view

King Fisher Duo Prime Magnetic Particle Processor

The KingFisher Duo magnetic particle processor has room for two plates and two elution strips. The

tip combs which protect the magnets during processing are compatible with the plates. During the

individual steps, the plates are kept stationary, and the only moving assembly is the processing head

with tip comb and magnetic rods. The head consists of two vertically moving platforms. One is needed

for the magnetic rods (12 or 6 pieces) and the other one for the plastic tip comb. For more detailed

information, see “Magnet Heads” on page 9, “Heating Blocks” on page 12, and “Elution Blocks” on

page 13.

Item number Description

1 Elution block (fixed, two pieces) and elution strip holder on top

2 Interchangeable heating block

3 Plate position

4 Positioning pin (to place the sample plate in the correct position)

5 Warning LEDs to indicate if there is a risk of burn

2 Functional Description

King Fisher Duo Prime Magnetic Particle Processor

Thermo Fisher Scientific Thermo Scientific KingFisher Duo Prime Technical Manual 7

You can place up to two plates simultaneously on the turntable and have them processed automatically

one after another. The processing of two sequential runs is started with the 1st plate with samples in a

96-well plate. Prepare the second sample plate while the first plate is still being processed, select the

protocol and place the plate onto the turntable. The processing of the second plate starts automatically

when the first one is completed. 24 samples at most can be purified continuously without an

interruption by the user, and in this case, the samples are processed in 96 deep well (DW) plates. When

using large-volume configurations on 24-well plates, usually two plates are used in a run.

Before starting the magnetic particle processing with the keypad and display, the samples and reagents

are dispensed into the plate(s)/strip(s) and the tip comb is placed onto the preset row of the sample

plate, default row B, from which it is automatically loaded. The plate(s) are placed onto the turntable

into the corresponding plate position according to the protocol instructions. During the operation, the

front lid can be closed or open (see Figure 2 on page 3 and Figure 3 on page 4). The closed door

protects the processing against environmental contamination.

Principle of Magnetic Particle Processing

The operating principle employed is inverse magnetic particle processing (MPP) technology (see Figure

6). Rather than moving the liquids, the magnetic particles are moved row wise on the plate(s) or

strip(s) containing specific reagents, in contrast to the external magnet method. Magnetic particles are

transferred using magnetic rods covered with a disposable, specially designed plastic tip comb.

Figure 6. Inverse magnetic particle processing

2 Functional Description

Working with Magnetic Rod

8Thermo Scientific KingFisher Duo Prime Technical Manual Thermo Fisher Scientific

Working with Magnetic Rod

Working with magnetic particles can be divided into five separate processes:

1. Collecting magnetic particles

2. Releasing magnetic particles

3. Washing magnetic particles

4. Incubation

5. Concentration

Collecting Magnetic Particles

During the collection of the magnetic particles, the magnetic rod is fully inside the tip. The magnetic

rods together with the tip comb move slowly up and down in the plate and the magnetic particles are

collected onto the edge of the tips. Having collected the magnetic particles, the magnetic rods together

with the tip comb can be lifted out of the wells and transferred into the next wells.

Releasing Magnetic Particles

After collection of the magnetic particles, the magnetic rods together with the tip comb are lifted from

the wells and transferred into the next wells containing the reagent, the magnetic rods together with

the tip comb are lowered into the wells and the magnetic rods are lifted off.

Magnetic particles are released by moving the tip comb up and down several times at considerably high

speed until all the particles have been mixed with the substance in the next reaction.

Washing Magnetic Particles and Incubation

Washing the magnetic particles is a frequent and an important processing phase. Washing is a

combination of the release and collection processes in wells filled with washing solution.

To maximize washing efficiency, the magnetic rods together with the tip comb are designed to have

minimized liquid-carrying properties.

To keep the magnetic particle suspension evenly mixed in incubating long-running reactions, the tip

comb can be moved up and down in the solution.

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Thermo Fisher Scientific KingFisher Duo Prime Owner's manual

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