Optech Basic Series User manual

Brand: Optech

Model: Basic Series

Category: Microscopes

Type: User manual

This manual is also suitable for

Biostar Series

18 ENGLISH

COMPLIANCE

DECLARATION CERTIFICATE

EXACTA+OPTECH GmbH

Munich - Germany

DECLARES:

That its OPTECH Microscopes comply with the latest versions

of the following Directives and with relevant legislation:

89/336/EEC Electromagnetic Compatibility Directive,

modified by 92/31/EEC and 93/68/EEC Directives;

and

EN 61010-1 (06-97).

EXACTA+OPTECH GmbH

ENGLISH 19

BIOSTAR AND BASIC

Biological microscopes

ILLUSTRATION LEGEND 20-21

GLOSSARY 20-21

BASIC SERIES 22-23

BIOSTAR SERIES 24-33

Dear Customer,

Thank-you for choosing an OPTECH product.

Please read this operation

and service manual carefully.

Used correctly, the precision instrument

you have purchased will provide many years

of trouble-free performance.

20 ENGLISH

19 20

21 22

Figure 1

ENGLISH 21

1Trinocular tube

2Head

3Head locking screw

4Stand

5Spring or spring-arm slide holder

6Limit stop adjustment screw

7Stage

8Condenser

9Condenser control knob

10 Spring-arm slide holder control knobs

11 Coarse/Fine control knob

12 Power on/off switch

13 Eyepiece

14 Dioptric compensation ring

15 Nosepiece revolver

16 Objective

17 Condenser centring screw

18 Condenser locking screw

19 Condenser diaphragm

20 Condenser filter holder ring

21 Collector filter housing

Illuminator collector diaphragm

23 Illuminator collector

24 Brightness control

ILLUSTRATION LEGEND

GLOSSARY (ref. fig. 1)

a) HEAD (2): Houses the eyepieces and allows the observation of specimens.

There are three types available: monocular, binocular or trinocular.

b) STAND (4): Base and body of the microscope that supports the head.

c) STAGE (7): Mechanical platform where the slide-mounted specimen is positioned.

Equipped with a spring-arm slide holder and features co-axial controls that adjust

the x-y movement of the specimen.

d) CONDENSER (8): System of converging lenses, which concentrates the light beam

onto the specimen.This light is formed into a cone that projects into the objective

lens; it is provided with a diaphragm called the condenser diaphragm (19), which

adjusts the light beam cone angle, and sometimes with an additional wide-field lens

to still further enlarge the beam. A filter holder ring (20), positioned at the base of

the condenser, allows a blue conversion filter to be inserted (except in model B5

where it is placed in the collector filter housing 21).

e) FINE-COARSE MOVEMENT (11): Coarse-fine focussing system.

f) EYEPIECE (13): Final image magnification lens system

g) REVOLVING NOSEPIECE (15): Mechanical rotating disc on which the objectives

are mounted.With sprung, slotted stops to ensure objective alignment.

h) OBJECTIVE (16): Primary image magnification lens system

i) ILLUMINATOR COLLECTOR (23):A system of lenses, positioned on the

stand’s base, which magnifies the light emitted by the lamp. It can be equipped with

a diaphragm called a collector diaphragm (22).

22 ENGLISH

BASIC SERIES

1. OPERATING YOUR OPTECH BASIC (ref. fig. 1)

1.a) After removing the protective packaging, insert the mains plug into a suitable supply

socket, checking that the voltage matches that indicated on the identification tag

situated at the rear of the stand (4).

1.b) Turn on using the power on/off switch (12).

1.c) Place the specimen slide on the stage (7), securing it using the spring slide holders (5).

1.d) Select the 4x objective (16), by rotating the nosepiece (15) until this objective locks

into its sprung slot

1.e) Using the coarse/fine focussing control knob (11) raise or lower the stage (7) until

the specimen comes into fine focus.

1.f) Search for the specific area to be observed, by gently moving the specimen slide

with your fingers.

1.g) If higher magnification is required, rotate the nosepiece (15) selecting the required

objective and refocussing, as necessary, using the coarse/fine control knob (11).

1.h) Search for the best balance between resolution, luminosity, contrast and depth of

field by rotating the disk condenser located under the stage (7).

2. CARE AND MAINTENANCE

2.1 Cleaning.

ATTENTION: always unplug the microscope before cleaning.

•To remove any dust on the lenses, use a soft bristle brush or lens cleaning tissue.

•To remove fingerprints or greasy marks from the lenses, use lens cleaning tissue or

a soft cloth soaked in a solvent such as xylene, benzene or ethyl alcohol.

•To clean the external, painted surfaces of the microscope, use a gentle detergent

and a soft cloth.

•We recommend that you always keep the microscope protected from dust after

use, a dust cover is provided for this purpose.

•Please do not use strong solvents to clean the external surfaces of the microscope;

they may damage the lacquer coating and plastic components.

ENGLISH 23

2.2 Lamp or fuse replacement.

ATTENTION: always unplug the microscope before replacing the lamp

or the fuse. Be very careful not to touch the new lamp with your fingers:

handle it using paper or clean tissue. Dirty lamps must be cleaned with

ethyl alcohol.

Unscrew the rubber feet, located under the microscope’s base, and take away the

protective panel.

Having replaced the detective lamp or fuse, replace the protective panel and screw the

rubber feet back on again.

2.3 Limit stop adjustment.

If focussing on the specimen at 400x magnification and the objective touches or

breaks the slide, please adjust the limit stop using the special screw (6 - ref. fig. 1).

OPTIONAL ACCESSORIES

MECHANICAL SLIDE CARRIER

Mechanical system that adjusts the x-y movement of the specimen.

Installation:

•Unscrew the spring slide holder (5 - ref. fig. 1) screws and take them away.

•Insert the mechanical slide carrier using the locating pins in it’s underside.

which mate directly into apertures in the standard stage.

•Tighten the locking screw.

STANDARD ACCESSORIES

•220V - 20W lamp.

•500mA 220V fuses (10 pcs.).

•Dust cover.

GUARANTEE

This instrument is guaranteed for 2 years from purchase date against component

failures and/or manufacturing defects. Please return the Guarantee Certificate, stamped

by your distributor, to our nearest branch office.

During the guarantee period, failures will be repaired at our branches free of charge,

except for carriage costs.

This guarantee is void if the instrument is put to any use other than that for which it

is intended, if it is damaged due to the negligence of the user,

or sustains accidental damage of any nature.

We reserve the right to introduce technical changes to the instruments without notice,

according to the evolution of the rules and the technology.

24 ENGLISH

Figure 2

BIOSTAR SERIES

1. MICROSCOPE ASSEMBLY (ref. fig. 1)

1.a) Remove the protective packaging of the stand (4) and of the head (2) and mount

the head onto the stand, being careful to tighten the head locking screw (3).

1.b) Carefully insert the eyepieces (13) into the head (2).

1.c) Firmly screw the objectives (16) into the nosepiece (15), positioning them,

clockwise, in increasing order of magnification.

1.d) Insert the blue conversion filter into the condenser filter holder ring (20).

In the case of models B5 and B5HP this filter has to be placed onto the collector

filter housing (21).

2. OPERATING YOUR OPTECH BIOSTAR

2.1 Set-up (ref. fig. 1)

2.1 a) Insert the mains supply plug into a suitable supply socket, checking that the voltage

matches that indicated on the identification tag situated at the rear of the stand (4).

2.1 b) Turn on using the power on/off switch (12).

2.1 c) Place the specimen slide on the stage (7), securing it with the spring-arm slide holder (5).

2.1d) Using the co-axial spring-arm slide holder control knobs (10), position the specimen

directly under the objective (16).

2.2 Focussing onto the specimen (ref. fig. 1)

2.2 a) Select the 10x objective by rotating the nosepiece revolver (15) until this

objective locks into it’s sprung slot.

2.2 b) Using the coarse/fine focussing control knob (11) raise or lower the stage (7)

until the specimen comes into focus, then use the fine focussing control knob to

obtain the clearest image.

2.2 c) Adjust the light intensity using the brightness control (24) until a clear, bright

image of the specimen is obtained.

2.2 d) If higher magnification is required, rotate the nosepiece revolver (15) selecting

the required objective and refocussing, if necessary, using the coarse/fine focussing

control knob (11).

2.3 Adjustment of the binocular or trinocular heads (2 - ref. fig. 1)

2.3 a) Adjust the interpupillary distance of the binocular

eyepiece tubes, unifying the right and left fields

of view (ref. fig. 2).

2.3 b) Looking only through the right eye

on the right eyepiece (13 - ref. fig. 1),

adjust the focus on the specimen using

the coarse/fine focussing control knobs

(11 - ref. fig. 1).

ENGLISH 25

Figure 3

2.3 c) Now looking only through the left eye and using the left eyepiece (13 - ref. fig. 1),

adjust the focus on the specimen using just the dioptric compensation ring (14 - ref. fig. 1).

In this way, both eyes will view the specimen together in perfect focus.

2.4 Condenser centring (7 - ref. fig. 1) (B4 and B5 models only)

2.4 a) Selecting the 4x objective, close the condenser diaphragm (19 - ref. fig. 1)

2.4 b) Lower the condenser (8 - ref. fig. 1) using the condenser control knob (9 - ref. fig. 1)

until, observing through the eyepieces (13 - ref. fig. 1),

there is a sharp image of the internal edges

of the condenser diaphragm (19 - ref. fig. 1).

2.4 c) If the internal edges are not in the centre of the viewing

field, centre the condenser (8 - ref. fig. 1) by adjusting

the condenser centring screws (17 - ref. fig. 1).

2.5 Using the condenser diaphragm.

Adjusting the condenser diaphragm (19 - ref. fig. 1)

ensures that the specimen image displays the best balance

between resolution, luminosity, contrast and depth of field.

We suggest opening the diaphragm to approximately 70-80% of it’s maximum

aperture (see fig. 3). If the objective is changed then the condenser diaphragm

setting must be re-adjusted accordingly.

3. CARE AND MAINTENANCE

3.1 Cleaning

ATTENTION: always unplug the microscope before cleaning.

•To remove any dust on the lenses, use a soft bristle brush or lens cleaning tissue.

•To remove fingerprints or greasy marks from the lenses, use lens cleaning

tissue or a soft cloth soaked in a solvent such as xylene, benzene or ethyl

alcohol.The same solvents can also be used to clean oil immersion objectives

(16 - ref. fig. 1) NOTE: please remember to remove oil from these objectives

every time you finish using them.

•To clean the external, painted surfaces of the microscope,

use a gentle detergent and a soft cloth.

•We recommend that you always keep the microscope protected from dust

after use, a dust cover is provided for this purpose.

3.2 Please do not:

•Remove the eyepieces (13 - ref. fig. 1) from the head, after fitting; dust ingress

could dirty prisms and internal lenses, reducing optical performance.

•Use strong solvents to clean the external painted parts of the microscope;

they may damage the lacquer coating and plastic components.

3. 3 Lamp replacement

(except for model B5HP)

ATTENTION:

always unplug the microscope

before replacing the lamp.

Be very careful not to touch

the new lamp

with your fingers:

handle it using paper

or clean tissue.

Dirty lamps must be cleaned with ethyl alcohol.

The lamp is located under the microscope’s stand (4 - ref. fig. 1). Referring to

figure 4, loosen the screw (4a) and open the housing cover. Replace the defective

lamp with a new one of equivalent specification.

After fitting the new lamp, close the cover and retighten the screw (4a).

3.4 Lamp centring.

Select the 4x objective (16 - ref. fig. 1), and then switch the illuminator on

(12 - ref. fig. 1). Looking through the eyepieces, you’ll note a luminous circle;

if it is not central within the field of view, referring to figure 4, proceed as follows:

3.4 a) Incline the microscope and loosen the screw (4b).

3.4 b) Place the microscope in the usual working position and move it away from the

support in order to be able to reach the screw (4b) with your hand.

3.4 c) Looking through the eyepieces, try to centre the luminous circle in the field of

view by gently moving the screw (4b) in all the directions until the correct,

centred position is attained.

3.4 d) Once the lamp has been centred, and continuing to view through the eyepieces,

carefully retighten the screw (4b) ensuring that the lamp remains steady throughout.

3. 5 Fuse replacement.

ATTENTION: always unplug the microscope before replacing the fuse.

The fuse is located in the housing under the microscope’s stand (4 - ref. fig. 1).

To r eplace it, loosen the fuse holder, remove the broken fuse and replace it

with a new one.Tighten the fuse holder again.

Figure 4

26 ENGLISH

Figure 5

MICRO-PHOTOGRAPHIC REFLEX

CAMERA DEVICE MODEL MF (ref. fig. 5).

Equipment:

T2-ring (5f); intermediate optic 2,5x-4x (5e);

WF 10x eyepiece with photographic graticule;

reflex camera body 24x36 (not supplied - available

on request).

a) Installation:

1) Having loosened the locking screw

(5b - ref. fig. 5) of the camera tube in the

trinocular head (1 - ref. fig. 1) and removed

the protective cover, mount the tube

containing the 2,5x-4x intermediate optic

(5e - ref. fig. 5).Tighten the locking screw

again (5b - ref. fig. 5).

2) Screw fit the T2 ring (5f - rif. fig. 5) to the

intermediate optic (5e - ref.fig.5), then lock

the reflex camera body onto the ring.

b) Preparation of the

micro-photographic device:

1) Substitute an eyepiece (13 - ref. fig. 1)

in the head with the WF 10x eyepiece with

photographic graticule.

2) Focus the photographic graticule using the

dioptric compensation ring of the same eyepiece.

ENGLISH 27

OPTIONAL ACCESSORIES

c) How to select the photographic optic 2,5x or 4x:

1) To get the 4x magnification, loosen the external screw (5d - ref. fig. 5) and reach

the 4x position. Lightly tighten the screw (5d - ref. fig. 5). Please check the lever

(5c - ref. fig. 1), positioned inside the photographic tube, is set to the 4x magnifying factor.

2) To get the 2,5x magnification, slide the lever (5c - ref. fig. 5), positioned inside the

photographic tube, to the 2,5x magnifying factor and lightly tighten the external

screw (5d - ref. fig. 5) on the 2,5x position.

d) How to take photos:

1) Place the specimen slide onto the stage (7 - ref. fig. 1) and, using the spring-arm

slide holder control knobs (10 - ref. fig. 1), search for the area to be photographed.

2) Focus on the specimen with high precision with the coarse/fine control knobs

(11 - ref. fig. 1).

3) Set the microscope illuminator to maximum intensity through the brightness

control (24 - ref. fig. 1).

4) Take the photo.

PLEASE NOTE:With prism slide knob (5a - ref. fig. 5) - equipped trinocular heads,

please remember to pull the slide knob outwards before taking the photo.

28 ENGLISH

Figure 6

ENGLISH 29OPTIONAL ACCESSORIES

MICRO-PHOTOGRAPHIC REFLEX CAMERA DEVICE MODEL MFM (ref. fig. 6)

Equipment: T2-ring (6d); intermediate optic 4X complete with focussing eyepiece (6c);

reflex camera body 24x36 (not supplied - available on request).

a) Installation:

1) Having loosened the locking screw (5b - ref. fig. 6) of the camera tube

(1 - ref. fig. 1) and removed the protective cover, mount the tube containing the

4x intermediate optic.Tighten the locking screw again (5b - ref. fig. 6).

2) Screw fit the T2 ring (6d - ref. fig. 6) to the intermediate optic, then lock the reflex

camera body onto the ring.

b) How to take photos:

1) Place the specimen slide onto the stage (7 - ref. fig. 1) and using the spring-arm

slide holder control knobs (10 - ref. fig. 1), search for the area to be photographed.

2) Focus the photographic graticule using the eyepiece dioptric compensation ring

(6c - ref. fig. 6) of the MFM micro-photographic device.

3) Focus on the specimen with high precision with the coarse/fine control knobs

(11 - ref. fig. 1).

4) Set the microscope illuminator to maximum intensity through the brightness control

(24 - ref. fig. 1).

5) Take the photo.

PLEASE NOTE:With prism slide knob (5a - ref. fig. 6) - equipped trinocular heads,

please remember to pull the slide knob outwards before taking the photo.

ADAPTER FOR DIGITAL CAMERAS

a) Installation:

1) Loosen the locking screw (5b - ref. fig. 6) of the trinocular tube in the head and

remove the protective cover.

2) Attach the adapter onto the camera.

3) Mount the digital camera, with the adapter, onto the trinocular tube,

tightening the locking screw (5b - ref. fig. 6).

b) How to take photos:

1) Place the specimen slide onto the stage (7 - ref. fig. 1) and using the spring-arm

slide holder control knobs (10 - ref. fig. 1), search for the area to be photographed.

2) Focus the photographic graticule using the eyepiece dioptric compensation ring

(6c - ref. fig. 6) of the micro-photographic device.

3) Focus on the specimen with high precision with the coarse/fine control knobs

(11 - ref. fig. 1).

4) Set the microscope illuminator to maximum intensity through the brightness control

(24 - ref. fig. 1).

5) Take the photo.

PLEASE NOTE:With prism slide knob (5a - ref. fig. 6)-equipped trinocular heads,

please remember to pull the slide knob outwards before taking the photo.

30 ENGLISH OPTIONAL ACCESSORIES

POSITIVE PHASE CONTRAST DEVICE

(models B4 and B5 only)

Equipment: phase contrast condenser (7a - ref. fig. 7)

phase objectives (7c - ref. fig. 7) - centring telescopic

eyepiece (7b - ref. fig. 7).

a) Installation:

a.1) Having loosened the fixing screw (18 - ref. fig. 1),

remove the brightfield condenser (8 - ref. fig. 1)

and insert the phase contrast condenser

(7a - ref. fig. 7). Retighten the screw (18 - ref. fig. 1)

again.

a.2) Replace the bright field objectives (16 - ref. fig. 1)

with phase contrast versions (7c - ref. fig. 7),

positioning them, clockwise, in increasing order

of magnification.

b) Centring:

1) Replace an eyepiece (13 - ref. fig. 1) with the

centring telescopic eyepiece (7b - ref. fig. 7)

supplied with the phase contrast device.

2) Using the 10x objective, set the phase contrast

condenser (7a - ref. fig. 7) to the number 10

position.Through the centring telescopic eyepiece

(7b - ref.fig. 7), check the phase rings are concentric,

as shown in figure 8a.

3) If the rings are not concentric (figure 8b), centre

them, carefully moving only the knurled ring

positioned in conjunction with the turret

(7a-1 - ref. fig. 7) of the phase condenser.

4) For centring the system when using higher

magnifications, repeat procedure 3) above,

remembering to always position the condenser

at the same number as the magnification value,

shown on the objectives (25x-40x-100x).

Figure 7

Figure 8a

Figure 8b

7a - 1

7b 7c

OPTIONAL ACCESSORIES ENGLISH 31

Figure 9a

Figure 9

9a - 1

POLARIZED LIGHT SYSTEM MODEL Pol 3

(for the stands slit equipped model only)

Equipment: Slide analyzer filter and polarizer filter.

Installation: Insert the slide analyzer filter in the special slit located above the nosepiece

revolver (15 - ref. fig. 1).

Now place the polarizer filter on the collector filter housing (21 - ref. fig. 1 and fig. 9a) and

increase the light intensity.

Rotate the polarizer filter until you view the greatest darkness in the viewing field and then

commence observation of the specimen.

DRY AND OIL DARK- FIELD

CONDENSERS

Installation:

Loosen the locking screw (18 - ref. fig. 1).

Replace the bright-field condenser (8 - ref. fig. 1)

with the dark-field version.

Retighten the locking screw (18 - ref. fig. 1).

It is important, before using the oil immersion

model, to put a drop of immersion oil between

the frontal lens of the condenser and the

specimen slide.

PLS. NOTE: Can be used up to a maximum

magnification of 400x.

POLARIZED LIGHT SYSTEM MODEL

Pol 2

Equipment: Analyzer and polarizer filters.

Installation: Referring to figure 1, having removed

the eyepieces (13), dismount the head (2) after

loosening the locking screw (3). In the base of

the head you will find the tube lens (9a-1 - ref. fig. 9)

which has to be unscrewed anti-clockwise.

Having removed the lens, insert the analyzer filter

and retighten the tube lens (9a-1 - ref. fig. 9).

Now place the polarizer filter on the collector

filter housing (21 - ref. fig. 1 and fig. 9a) and increase

the light intensity.

Rotate the polarizer filter until you view the

greatest darkness in the viewing field and then

commence observation of the specimen.

32 ENGLISH OPTIONAL ACCESSORIES

CCD

VIDEOCAMERA ADAPTER

a) Installation:

a.1) Remove the protective cover of the trinocular

head (1 - ref. fig. 1) loosening the locking screw

(5b - ref. fig. 10).

a.2) Screw the adapter to the videocamera.

a.3) Mount the video camera, complete with VS

adapter, onto the head, tightening the locking

screw (5b - ref. fig. 10).

b) Operation:

b.1) We suggest that the blue conversion filter is

used, inserting it into the microscope’s condenser

filter holder ring (20 - ref. fig. 1).

b.2) Keep the microscope illumination low to avoid a

colour saturation on the monitor.

b.3) Try to focus on the specimen as finely as possible

using the coarse/fine control knobs (11 - ref. fig. 1).

b.4) Observe the video screen.

PLEASE NOTE:

With prism slide knob (5a - ref. fig. 10) - equipped

trinocular heads, please remember to pull the slide

knob outwards before looking at the video screen.

10c

10d

Figure 10

ENGLISH 33

STANDARD ACCESSORIES

a) Instruction manual

b) Spare halogen lamp: 6V 20W (model B3 - B4 - B5) - 12V 50W (model B5HP).

c) Spare fuse 500mA.

d) Blue conversion filter.

e) Bottle of immersion oil.

f) Dust cover.

g) Protective packaging.

SPARE PARTS

•Spare halogen bulb 6V 10W (Model B3) - Code K7169

•Spare halogen bulb 6V 20W (Models B3 - B4 - B5) - Code K71232

•Spare halogen bulb 12V 50W (Model B5HP) - Code K71415

•Fuses (set 10 pcs. 500mA 220V) - Code F719915

•Optical cleaning tissues (pack of 25 pcs.) - Code F5705

•Objective cleaning solution - Code E66996

•Blue conversion filter - Code F719922

•7 ml bottle of immersion oil - Code K71413

•Dust cover (Models B3 - B4 - B5) - Code F719921

•Dust cover (Model B5HP) - Code F719926

GUARANTEE

This instrument is guaranteed for 2 years from purchase date against component

failures and/or manufacturing defects. Please return the Guarantee Certificate, stamped

by your distributor, to our nearest branch office.

During the guarantee period, failures will be repaired at our branches free of charge,

except for carriage costs.

This guarantee is void if the instrument is put to any use other than that for which it

is intended, if it is damaged due to the negligence of the user, or sustains accidental

damage of any nature.

We reserve the right to introduce technical changes to the instruments without notice,

according to the evolution of the rules and the technology.

Optech Basic Series User manual

Brand: Optech

Model: Basic Series

Category: Microscopes

Type: User manual

This manual is also suitable for

Biostar Series

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Optech Basic Series User manual

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Optech Basic Series User manual

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