Thermo Fisher Scientific Network Port Calibration and StepOne™ Owner's manual

Brand
Thermo Fisher Scientific
Model
Network Port Calibration and StepOne™
Type
Owner's manual
User Bulletin
Applied Biosystems StepOne™ /StepOnePlus™ Real-Time PCR System
July 30, 2007
SUBJECT: Network Port Calibration and StepOne™ Instrument
Connection Procedures
In This User
Bulletin
This user bulletin covers the following topics:
•”How to Check that the Network Port of the Computer Is Set to DHCP” on
page 1
•”How to Check that the StepOne™ Instrument Communicates with the
Computer” on page 6
How to Check
that the Network
Port of the
Computer Is Set
to DHCP
If your computer does not connect to the StepOne™ instrument, you must check that
the network port on the computer is set to DHCP (to automatically obtain the IP
address).
To check the status of your computer network port:
1. Disconnect your computer from the StepOne™ instrument and the Local Area
Network.
2. Unplug all of the Ethernet cables from the computer.
3. From the desktop, select StartControl Panel.
2Applied Biosystems StepOne™/StepOnePlus™ Real-Time PCR System User Bulletin
Network Port Calibration and StepOne™ Instrument Connection Procedures
4. In the Control Panel window, double-click Network Connection.
.
5. In the Network Connections window, double-click the appropriate network port.
.
3Applied Biosystems StepOne™/StepOnePlus™ Real-Time PCR System User Bulletin
6. In the Local Area Connection Properties dialog box, select Internet Protocol
(TCP/IP), then click Properties.
.
7. In the General tab of the Internet Protocol (TCP/IP) Properties dialog box, select
Obtain an IP Address automatically.
.
4Applied Biosystems StepOne™/StepOnePlus™ Real-Time PCR System User Bulletin
Network Port Calibration and StepOne™ Instrument Connection Procedures
8. Select the Alternate Configuration tab, select Automatic private IP address,
then click OK.
.
9. From the Local Area Connection Properties, click Close.
10. Connect your computer to the StepOne™ instrument:
a. Connect the yellow Ethernet cable to the yellow Ethernet port on the
StepOne™ instrument.
b. Connect the other end of the yellow Ethernet cable to the integrated
Ethernet port on the computer, the same Ethernet port that corresponds to
the Local Area Network connection configured in steps 5 through step 9.
11. Power on the StepOne™ instrument. Wait for the main menu to appear.
5Applied Biosystems StepOne™/StepOnePlus™ Real-Time PCR System User Bulletin
12. In the computer desktop, select StartControl PanelNetwork
Connections.
13. In the Network Connections window, double-click the same network port as in
step 5 on page 2.
14. In the My Local Area Connection Status dialog box, select Support tab, then
verify that the:
a. Address type is Assigned by DHCP.
b. StepOne ™ instrument has sent an IP address to the computer.
c. IP address begins with 192.168.0
a
a
b, c
a
6Applied Biosystems StepOne™/StepOnePlus™ Real-Time PCR System User Bulletin
Network Port Calibration and StepOne™ Instrument Connection Procedures
How to Check
that the
StepOne™
Instrument
Communicates
with the
Computer
You can now verify the connection between the computer and the StepOne™
instrument by “pinging” the StepOne™ instrument.
To ping the StepOne™ instrument:
1. From the computer desktop, select StartRun to open the DOS window.
2. Enter cmd in the command line, then click OK.
3. After the DOS window opens, wait 15 sec.
4. At the DOS prompt, enter ping 192.168.0.1,then wait for the response.
7Applied Biosystems StepOne™/StepOnePlus™ Real-Time PCR System User Bulletin
The following screen is displayed if the computer is communicating with the
StepOne™ instrument.
If there is no communication, take a screen capture of the screen on step 14 on
page 5, then send it to your Applied Biosystems contact.
www.appliedbiosystems.com
Worldwide Sales and Support
Applied Biosystems vast distribution and
service network, composed of highly trained
support and applications personnel,
reaches 150 countries on six continents.
For sales office locations and technical support,
please call our local office or refer to our
Web site at www.appliedbiosystems.com.
Applera is committed to providing the
world’s leading technology and information
for life scientists. Applera Corporation
consists of the Applied Biosystems and
Celera Genomics businesses.
Headquarters
850 Lincoln Centre Drive
Foster City, CA 94404 USA
Phone: +1 650.638.5800
Toll Free (In North America): +1 800.345.5224
Fax: +1 650.638.5884
07/2007
Part Number 4391120 Rev. A
Stock Number 117UB29-01
© Copyright 2007, Applied Biosystems. All rights reserved.
Information in this document is subject to change without notice. Applied Biosystems assumes no
responsibility for any errors that may appear in this document.
APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT,
EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY
OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL APPLIED BIOSYSTEMS BE
LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY
OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR
CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,
INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
The StepOne™ and StepOnePlus™ Real-Time PCR System are covered by patents owned by
Applera Corporation. No right is conveyed expressly, by implication, or by estoppel to apparatus,
reagents, kits, or methods such as the patented 5í nuclease methods. For further information,
contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City,
California 94404, USA.
TRADEMARKS:
Applera, Applied Biosystems, and AB (Design) are registered trademarks, and StepOne™ and
StepOnePlus™ are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain
other countries.
Microsoft and Windows are registered trademarks of Microsoft Corporation.
All other trademarks are the sole property of their respective owners.
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Thermo Fisher Scientific Network Port Calibration and StepOne™ Owner's manual

Brand
Thermo Fisher Scientific
Model
Network Port Calibration and StepOne™
Type
Owner's manual
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