Kyowa Medex Co., Ltd. HM-JACKarc Operating instructions

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i

Fully Automated FOB analyzer: HM-JACK series

FECAL OCCULT BLOOD TEST ANALYZER

For Professional Use only

Operation Manual（Rev.4）

Kyowa Medex Co., Ltd.

IVD

HM-JACKarc Operation Manual

Revision Record

ii Rev.4

Revision Record

Manual Title : Fecal Occult Blood Analyzer HM-JACKarc Operation Manual

Edition

Printed Date

Revised Pages

Description

Rev.1

Dec. 2011

Newly Released

Rev.2

Jun. 2014

－

Wholly Revised

Updated to Main Program Ver.3.01

Rev.3

Feb.2016

6.3

Added some description to

“List of the Last three Digits of Error Codes”

Rev.4

Oct.2017

7.1

Added an item to specifications of Power supply

Fully Automatic Human Fecal Occult Blood Hemoglobin Analyzer HM-JACKarc Operation Manual

First edition published: December 2011

Written and edited by: Medical Instrument Department, Kyowa Medex Co., Ltd.

Published by: Kyowa Medex Co., Ltd.

1-8-10 Harumi, Chuo-ku, Tokyo 104-6004

HM-JACKarc Operation Manual

Contents

iii Rev.4

Contents

1 Introduction ........................................................................................ 1-1

 Warranty ................................................................................................................... 1-1

 Disclaimers ............................................................................................................... 1-1

 Applicable laws and standards ................................................................................. 1-2

 Caution ..................................................................................................................... 1-2

 Restrictions .............................................................................................................. 1-2

 Prohibition of Modification ........................................................................................ 1-3

 Contact ..................................................................................................................... 1-3

 Other manuals .......................................................................................................... 1-3

 Conventions employed in this Manual ..................................................................... 1-4

Ministry of Health and Welfare Guidelines on Medical Instruments .... 1-5

2 Safety Precautions ............................................................................ 2-1

2.1 Explanation of Alerting Symbol ...................................................................................... 2-1

2.2 Explanation of Warnings ................................................................................................ 2-1

2.3 Explanation of Warning Labels Affixed to the Analyzer ................................................. 2-2

2.4 Safety Precautions for Operators .................................................................................. 2-3

2.5 Safety Precautions for Maintenance .............................................................................. 2-5

2.6 Waste Materials Precautions. ........................................................................................ 2-6

2.7 Instrument Safety Device. ........................................................................................... 2-7

2.8 Noise Level of the Analyzer. ...................................................................................... 2-7

2.9 Note for Supplied Water and Detergent. ................................................................... 2-7

3 Outline of Analyzer ............................................................................ 3-1

3.1 Characteristics of the Analyzer ...................................................................................... 3-1

3.1.1 Outline .............................................................................................................. 3-1

3.1.2 Principles of Operation ....................................................................................... 3-2

3.1.3 Stool Samplers and Reagents ............................................................................ 3-4

3.2 Part Names .................................................................................................................... 3-5

 Top ........................................................................................................................... 3-5

 Right side ................................................................................................................ 3-5

 Left side ................................................................................................................... 3-6

 Back ........................................................................................................................ 3-6

3.3 Materials Required ....................................................................................................... 3-7

HM-JACKarc Operation Manual

Contents

iv Rev.4

4 Function of Each Screen ................................................................ 4-1

4.1 Display and Operation Screen ................................................................................... 4-1

4.1.1 Startup screen (initial screen) ......................................................................... 4-1

4.1.2 Main Menu screen .......................................................................................... 4-1

4.1.3 Order .............................................................................................................. 4-4

4.1.4 Analysis .......................................................................................................... 4-7

4.1.5 Cell set .......................................................................................................... 4-10

4.1.6 Calibration .................................................................................................... 4-12

4.1.7 Result ........................................................................................................... 4-14

4.1.8 Reaction Data ............................................................................................... 4-16

4.1.9 QC ................................................................................................................ 4-17

4.2 System Menu Screen ............................................................................................ 4-19

4.3 Maintenance Menu Screen .................................................................................... 4-20

5 Analysis Operations ....................................................................... 5-1

5.1 Flow of Analysis Operations ....................................................................................... 5-1

5.2 Preparations for Starting Analysis .............................................................................. 5-2

5.2.1 Turning on the Analyzer ................................................................................. 5-2

 Turning on manually ................................................................................ 5-2

 Turning on automatically (from Sleep Mode) .......................................... 5-2

 Logging in to the Analyzer ....................................................................... 5-3

5.2.2 Preparing Detergent in the Detergent Tank .................................................... 5-4

5.2.3 Preparing the Waste Tank .............................................................................. 5-5

5.2.4 Preparing the Reagent ................................................................................... 5-6

 Setting the Reagent in place ................................................................... 5-6

 Setting Reagent volume .......................................................................... 5-7

5.2.5 Preparing the Buffer ....................................................................................... 5-9

5.2.6 Preparing the Reaction Cell ......................................................................... 5-12

5.2.7 Registration of Master Curve and Calibration Card ..................................... 5-14

5.2.8 Preparation of Samples ................................................................................ 5-15

 Definition of Racks ................................................................................ 5-15

 Preparation of Calibrator ....................................................................... 5-15

 Preparation of Control ........................................................................... 5-16

 Preparation of Patient Sample .............................................................. 5-17

5.2.9 Loading the Racks to the Analyzer ............................................................... 5-19

 Rack Orientation .................................................................................... 5-19

 Loading Racks to the Analyzer .............................................................. 5-19

5.3 Analysis .................................................................................................................... 5-21

 Adding samples during analysis ............................................................ 5-21

 Next batch analysis ............................................................................... 5-21

 Handling Results ................................................................................... 5-22

5.4 Shut Down Procedure ............................................................................................ 5-23

HM-JACKarc Operation Manual

Contents

v Rev.4

5.4.1 Clearing up after completing analysis .......................................................... 5-23

5.4.2 Sleep Mode/Switch off.................................................................................. 5-24

■ Sleep Mode ........................................................................................... 5-24

 Switching off .......................................................................................... 5-24

5.5 Supplemental Operations ........................................................................................ 5-25

5.5.1 Reading Barcodes with Handheld Scanner ................................................ 5-25

5.5.2 Inputting information and data manually..................................................... 5-26

■ Clearing input protect ............................................................................ 5-26

 Inputting data for Calibrator ................................................................... 5-27

 Inputting I.S.T of Master Curve ............................................................. 5-27

 Inputting Expiration information of Master Curve .................................. 5-28

 Inputting Lot information of Master Curve ............................................. 5-28

5.5.3 Setting the first position of picking up the Cell ............................................ 5-30

5.5.4 Setting Printer Paper .................................................................................. 5-31

5.5.5 Actions for Recovery from Emergency STOP ............................................ 5-33

6 Error Messages .............................................................................. 6-1

6.1 Structure of the Codes ............................................................................................... 6-1

6.2 List of the First Two Digits .......................................................................................... 6-2

6.3 List of the Last Three Digits of Error Codes ............................................................... 6-3

6.4 Trouble Shooting Actions (for trained personnel only) ............................................... 6-7

7 Miscellanea .................................................................................... 7-1

7.1 Specifications of the Analyzer .................................................................................... 7-1

7.2 Outline Dimensional Drawing..................................................................................... 7-2

7.3 Communication Specification ..................................................................................... 7-3

7.4 Tube Connection Diagram ....................................................................................... 7-4

7.5 Wiring Connection Diagram ....................................................................................... 7-5

HM-JACKarc Operation Manual

Introduction

1 - 1 Rev.4

１ Introduction

Thank you for choosing the Fully Automatic Human Fecal Occult Blood

Hemoglobin Analyzer HM-JACKarc (referred to below as the Analyzer

or HM-JACKarc).

The Operation Manual (referred to below as the Manual) is intended for

use by those who will operate and maintain the Analyzer.

Those intended should read the Manual thoroughly, and not perform

analysis operation and/or maintenance management of the Analyzer

until they fully understand its content.

Keep this Manual near the Analyzer. If you are unsure of the analysis

operation method for this Analyzer, read the Manual thoroughly before

proceeding with any operation or analysis.

Store this Manual carefully.

 Warranty This Manual should be kept where it will be readily available to those

who use the Analyzer.

 The content of the Manual and software is subject to change without

notice.

Warranty period Refer to local distributor.

Warranty coverage During the warranty period, all malfunctions due to manufacturing or

design faults will be repaired free of charge.

Exemptions The following are not covered by warranty even if they occur within the

warranty period.

1. Malfunctions due to disassembly or modifications not connected with

Kyowa Medex.

2. Malfunctions caused by operating methods not described in this

Manual.

3. Accidents caused by fire, lightning strike, earthquakes, wind, flooding,

riots, crime, war, or other force majeure.

4. Malfunctions caused by using parts or software not connected with

Kyowa Medex.

5. Malfunctions of consumable parts or using parts outside their period

of validity.

 Disclaimers Kyowa Medex bears no liability in the event of losses to the customer or

any third party through the use of this Analyzer, or losses to the

customer or any third party through defects in this Analyzer that could

not be foreseen by the company.

HM-JACKarc Operation Manual

Introduction

1 - 2 Rev.4

 Applicable laws and standards

This Analyzer falls under a medical analyzer as specified by the

Pharmaceutical Affairs Act of Japan.

Medical instrument manufacturing license number: 22B3X00004

With regard to the electrical safety test, this is a Class I device of Type

B as specified by JIS T1001 and IEC 61010-1, 2.

 Caution This Manual should be kept where it will be readily available to those

who use this Analyzer.

 The content of this Manual and software is subject to change without

notice.

 In the event of any inconsistencies between this document and the

software, the software is to take precedence.

 All other company names and product names used in this Manual

are trademarks or registered trademarks of their respective

companies.

 Reproduction of all or part of this Manual without prior consent is

prohibited.

 The content of this Manual is subject to change without notice.

While we have taken all possible precautions to ensure quality in the

content of this Manual, please contact your dealership or contact Kyowa

Medex Co., Ltd. if you find anything unclear, or any apparent errors or

omissions. Our contact details are stated on the last page of this

Manual.

This Manual is copyrighted by Kyowa Medex Co., Ltd.

 Restrictions  Copying of the HM-JACKarc application software without prior

consent is prohibited.

 We do not warrant the functionality of the HM-JACKarc control

computer when used for other purposes.

 Do not install any other application software on the HM-JACKarc

control computer. We do not warrant the functionality of the PC if any

other applications are installed.

 Do not start any other application while the HM-JACKarc application

is running. We do not warrant the functionality if any other

application is running at the same time.

 We do not warrant measurement data that is accidentally deleted.

 Password-based restriction is provided for functions that are not

required in normal operation.

HM-JACKarc Operation Manual

Introduction

1 - 3 Rev.4

 Prohibition of Modification For the safety of the operator, and to protect the Analyzer, handle the

Analyzer according to the instructions regarding safety precautions in

this Manual.

And please refrain from modifying this Analyzer.

 Contact Kyowa Medex Co., Ltd.

1-8-10 Harumi, Chuo-ku, Tokyo Japan 104-6004

Tel: +(81)-3-6219-7605 Fax: +(81)-3-6219-7614 [email protected]

Obelis S.A.

Boulevard Général Wahis 53, Brussels, BELGIUM 1030

Tel +(32)2.732.59.54 Fax +(32)2.732.60.03 e-mail: [email protected]

 Other manuals Other than this Manual read the following operation manuals thoroughly

and be sure to understand the information before performing analysis

operations or handling the reagent and other materials.

Keep the Manual near the Analyzer. If you are unsure of the analysis

operation method for this Analyzer, read this Manual thoroughly before

going on with operation and analysis.

Store this Manual carefully.

Operation Manuals packed with this Analyzer

 CCD Touch Scanner FFTA10A Operation Manual

Corresponding codes: CODE39, ITF, Industrial 2 of 5, COOP2 of 5,

NW-7, CODE128, JAN

Instructions packed with other supplies indispensable

 EXTEL HEMO・AUTO HS Instructions for Use

 EXTEL HEMO・AUTO HS Calibrator Instructions for Use

 EXTEL HEMO・AUTO MC Collection Picker Instructions for Use

HM-JACKarc Operation Manual

Introduction

1 - 4 Rev.4

 Conventions employed in this Manual

Handling precautions: This Manual indicates information on precautions for handling the

Instrument as "Handling Precautions".

Carefully read the information following "Handling Precautions", and

handle the Analyzer accordingly.

: This Manual indicates information that the user should be particularly

aware of when performing analysis operation or maintenance

management tasks as "Request".

 : Indicates section to reference in this Manual.

* : Provides supplementary information.

1. Numbers in italics in the text indicate the step numbers in procedures

and the sequences of operations. Perform operations and tasks

according to these numbers.

Request

HM-JACKarc Operation Manual

Introduction

1 - 5 Rev.4

Ministry of Health and Welfare Guidelines

on Medical Instruments

The attachment of this document, Precautions for the Use of Medical Electronic Instruments (for

Safety and Risk Prevention), to medical electronic instruments is required under Notification No. 495

from the Head of Pharmaceutical Affairs Bureau (June 1st, 1972). These precautions should be

observed to handle the Instrument correctly when it is in use.

Precautions for the Use of Medical Electronic Instruments

(for Safety and Risk Prevention)

1. Only the experienced staff are permitted to use the Instrument.

2. The following points must be observed when installing the Instrument.

(1) Install the Instrument in a location where it is not exposed to water.

(2) Install the Instrument in a location where there is no risk of adverse influence from

atmospheric pressure, temperature, humidity, drafts, sunlight, dust, air containing

substances such as salinity, sulfur, etc. or other environmental factors.

(3) Make sure the Instrument is safe from tilting, vibration and impact (including that during

movement).

(4) Do not install the Instrument in a location where chemical substances are stored or where

gas is generated.

(5) Pay close attention to the voltage and frequency of the power supply, and to the allowable

current (or consumption power).

(6) If battery-based power supply is used, check the status of the battery (depth of discharge

and polarity), etc.

(7) The Instrument must be correctly grounded.

3. The following points must be observed before using the Instrument.

(1) Check that the Instrument functions correctly.

(2) Check that all cables are connected correctly and securely.

(3) Be careful of using the equipment in combination with other instruments that can induce

errors in correct diagnoses and cause hazards.

(4) Check the battery power supply, if one is used.

HM-JACKarc Operation Manual

Introduction

1 - 6 Rev.4

4. The following points must be observed after using the Instrument.

(1) Follow the set procedure to return all control switches, dials to the same positions they

had before the Instrument was used, and then turn off the power.

(2) When you disconnect any cables, do not apply any excessive or inappropriate forces on

them, such as pulling them by the cable rather than by the plug.

(3) Pay attention to the following points regarding the storage location.

i) Store the Instrument in a location where it is not exposed to water.

ii) Store the Instrument in a location where there is no risk of adverse influence from

atmospheric pressure, temperature, humidity, drafts, sunlight, dust, air containing

salinity and/or sulfur or other environmental factors.

iii) Make sure the Instrument is safe from tilting, vibration and impact (including that

during movement).

iV) Do not store the Instrument in a location where chemical substances are stored or

where gas is generated.

(4) Clean the accessories, cables and probes.

(5) Be sure to keep the Instrument clean.

5. If the Instrument breaks down, do not attempt to fix it. Label it appropriately as "out of service" and

contact a specialist for repair.

6. Do not modify the Instrument.

7. Maintenance and inspections

(1) Inspect the Instrument and its components regularly.

(2) Before resuming use of the Instrument that has not been used for some time, be sure to

check that it operates correctly and safely.

8. In addition

The Instrument must be operated correctly, in accordance with the operation manuals.

HM-JACKarc Operation Manual

Safety Precautions

2 - 1 Rev.4

2 Safety Precautions

2.1 Explanation about Alert Symbols

The alert symbol on the left is used on this Instrument and in this

Manual to indicate potential dangers. This symbol represents

unspecified general risks and provides warnings and cautions. The

precautions stated after this symbol should be notified for safety

reasons.

The alert symbol on the left used on the Instrument and in this Manual

indicates potential dangers. This symbol represents infectious risk and

provides warnings and cautions. The precautions stated after this

symbol should be notified for safety reasons.

2.2 Explanation of Warnings

Two types of warning legends are used on the Instrument and in this

Manual. These warnings are used separately as described below to

indicate the degree (magnitude) of personal injury and property damage

which could occur.

 This signal and the legend is used in situations in which

incorrect handling could result in serious injury to the user.

 This signal and the legend is used in situations in which incorrect

handling could result in minor injury to the user.

Classifications and categories of damage and injury severity

The degrees of damage and injury used above are classified as follows:

Severe Injury: Loss of vision, wounds, burns (high or low

temperature), electric shock, bone fracture, poisoning,

infection etc. resulting in permanent after effects, or

requiring medical care involving hospitalization or

prolonged outpatient treatment.

Light injury: Injury other than the above, not requiring medical care

involving hospitalization or prolonged outpatient

treatment.

Warning

Caution

HM-JACKarc Operation Manual

Safety Precautions

2 - 2 Rev.4

2.3 Explanation of Warning Labels Affixed to

the Analyzer

 Make sure that warning labels are legible at all times. If a label peels

off, contact local distributor immediately.

 Never peel warning labels off or modify them.

The warning labels shown below are affixed to warn operators of

potential hazards.

Caution

HM-JACKarc Operation Manual

Safety Precautions

2 - 3 Rev.4

2.4 Safety Precautions for Operators

 Before using this Instrument, make sure that the Instrument

was installed by authorized personnel.

 If the Instrument is not installed correctly, it could break down

or cause fire, electric shock or injury.

 Check the following points before starting operation. These

checks are necessary in order to prevent accidents and injuries.

the warning labels are legible

the ground connection is fully secured

 A broken power cable could result in electric shock, fire, and

short circuit. Observe the following points.

the cable is not twisted

the cable is not caught by pressure between the Instrument

and the wall.

the cable is not bent too tight

nothing is put on the cable

the cable is not tied in a bundle

the cable is not modified

the cable is kept away from any heater.

the plug itself, not the cable, is gripped when unplugging the

cable

 Dust accumulated on the power plug can start a fire. Check the

plug regularly and remove dust.

 Insert the power plug firmly into the socket. Poor contact could

cause overheating, burn injuries and fires.

 Do not get the power cable or plug wet, or electrical shock and

short circuit may result.

 Never touch the power plug with wet hands, or electric shock

may result.

 Using the power cable plug (plugging it in and unplugging it) to

turn the power on and off may cause the plug to overheat.

Always use the power switch (or the Main Breaker).

Warning

HM-JACKarc Operation Manual

Safety Precautions

2 - 4 Rev.4

 Only use consumables and replacement parts (including

screws) that are specified by Kyowa Medex Co., Ltd.

 Before cleaning the Instrument, turn the power off and be

sure to remove the power plug from the socket.

 In the event of any malfunction of the Instrument, turn the

power switch off immediately and remove the power plug

from the socket. Leaving faults unattended can cause

accidents and fires.

 Do not turn the power switch off and on repeatedly.

 The Mixing Spatula, Sample Pipette and Reagent Pipette

attached to the injection assembly are sharp and could

cause injury.

 The ends of the Mixing Spatula, Sample Pipette and Reagent

Pipette attached to the injection assembly come into direct

contact with Samples (stool samples) and Reagent. Incorrect

handling of those parts could cause infection with

pathogens or poisoning with Reagent. Wear medical rubber

gloves while working, and take extra care to avoid infection.

Warning

HM-JACKarc Operation Manual

Safety Precautions

2 - 5 Rev.4

2.5 Safety Precautions for Maintenance

 Do not modify the Instrument without permission. Modification

could cause unexpected hazards.

 Use consumables and replacement parts (including screws)

only specified by Kyowa Medex Co., Ltd.

 Before servicing the Instrument or removing any of its parts

(fixing faults, replacing parts, inspecting, repairing, etc.), turn

the power switch off and be sure to disconnect the power plug

from the socket to avoid getting electric shock.

 When disconnecting the power plug from the socket, always

grip and pull on the plug itself, not on the cable.

 Use fuses of the only specified type. Use of non-specified fuses

could cause the Instrument to malfunction or, in some cases,

start a fire.

 Using the power cable plug (plugging it in and unplugging it) to

turn the power on and off may cause the plug to overheat.

Always use the power switch (or the Main Breaker).

 This Instrument weighs approximately 56 kg. Handle it with

extra care when moving. Note that moving the Instrument

should be done by two or more people.

 The ends of the Mixing Spatula, Sample Pipette and Reagent

Pipette attached to the injection assembly are sharp and could

cause injury.

 The ends of the Mixing Spatula, Sample Pipette and Reagent

Pipette attached to the injection assembly come into direct

contact with Samples (stool samples) and Reagents, so

incorrect handling of those parts could cause infection with

pathogens or poisoning with reagents. Wear medical rubber

gloves while working, and take extra care to avoid infection.

Warning

HM-JACKarc Operation Manual

Safety Precautions

2 - 6 Rev.4

2.6 Waste Materials Precautions

 Samples could be contaminated with pathogens etc., so take

extra care to avoid infection when handling them, by wearing

medical rubber gloves etc.

 Before handling sample containers, reagents and buffer, read

and understand the Instructions for Use. Handle them with care

to avoid accidents.

 Any Mixing Spatula, Sample Pipette and Reagent Pipette which

has been detached or removed is sharp and dangerous. Handle

them with extra care.

 The ends of the Mixing Spatula, Sample Pipette and Reagent

Pipette attached to the injection assembly come into direct

contact with samples (stool samples) and reagents. Incorrect

handling of those parts could cause infection with pathogens or

poisoning with reagents. Wear medical rubber gloves at all

times while working, and take extra care to avoid infection.

 Here below is a list of Waste materials generated by regular analysis.

They should be handled as medical waste and disposed of by a

specialist contractor.

 Liquid waste

 Used cells

 Reagents and reagent bottles

 Buffer and buffer fluid bottles

 Used stool sample containers

 Hemoglobin standards before and after preparation

 Hemoglobin standard containers

 Used sample cups

 Control samples before and after preparation

 Control sample containers

 The Instrument contains lithium battery. The local restriction or WEEE

should be applied when battery is wasted. Ask for local distributor for

more information.

Warning

Caution

HM-JACKarc Operation Manual

Safety Precautions

2 - 7 Rev.4

2.7 Instrument Safety Device

 Overcurrent Protection

2 or more ampere of current (input voltage 220v) will be interrupted

immediately by internal breaker and the power will be shut down.

 Transformer Secondary Fuses

 A fuse is installed to protect Control PCBs (Main PCB, Power PCB) from

overcurrent.

 Thetransformer is protected from overheating by a thermal fuse.

 A fuse is installed to protect the lamp from overcurrent.

 Outer Cover Opening Check Sensor

If the outer cover is open, the Analyzer does not start motion. If the outer

cover is opened while the Analyzer is in motion, an error occurs and the

Analyzer will stop sampling. There are 2 switches to secure the function.

2.8 Noise Level of the Analyzer

The maximum noise level is 70db.

2.9 Note for Supplied Water and Detergent

 Temperature of supplied water

The temperature of supplied water for this Instrument should be in

20-30 ℃. Lower temperature of water may cause malfunction of

instrument wash, and higher one may damage the tubing and lead to

leakage of water.

 Amount of use of Detergent

The amount of use of Detergent (water include 0.1% of detergent) is

approximately 4.5mL per a test. It is also used in priming and wash. It is

necessary to keep enough amount of prepared Detergent during the run.

HM-JACKarc Operation Manual

Outline of Instrument

3 - 1 Rev.4

3 Outline of Analyzer

3.1 Characteristics of the Analyzer

3.1.1 Outline

 This Analyzer measures the amount of human hemoglobin present in

a stool sample.

 Compact and light analyzer designed for installation in a small space.

 Barcode reading input is applied to register Sample ID, Calibration

Master Curve and Calibrator Information to make the operation easier

for operators.

 A two-point calibration method is adopted to simplify the measurement

operation.

 The measurement method is Latex Agglutination, which is quick and

achieves superior sensitivity.

 Superior detection sensitivity realizes smaller volume of stool sample

and less smell.

 The sample loading capacity of the Analyzer is 80.

 Reaction Cells are replaced after use automatically.

 The Sample Collection Picker achieves superior stability for human

hemoglobin, reduces the decline in the amount of human hemoglobin

and enables more accurate measurement.

 Analysis results can be checked on the screen and printout. Also,

connection to a host computer enables data to be exported.

HM-JACKarc Operation Manual

Outline of Instrument

3 - 2 Rev.4

3.1.2 Principles of Operation

The Reagent Pipette aspirates the Latex Reagent, which then move to a

Cell on the Reaction Table, where it is dispensed together with the Buffer.

Next, the Reaction Table Rotates and the Cell containing the Reagent

moves to the sampling position. The Sample Pipette aspirates a Sample,

which is then dispensed to the Cell. The Mixing Spatula then turns

counterclockwise to mix the Reagent and the Sample together.

The Cell containing the Reagent and the Sample moves to the detection

position after a period of time set under the measurement conditions, and

the first photometric measurement (ISTT1) is performed. After another set

period of time, the second photometric measurement (ISTT2) is

performed.

The amount of change in the Integrating Sphere Turbidity of the two

photometric measurements (ΔIST=ISTT2-ISTT1) is calculated by the

computer according to the calibration curve to output the measurement

result.

This series of actions is repeated to measure all samples placed in the

sample rack.

IST: Integrating Sphere Turbidity

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