WPI SP101i Two Syringe Infusion Pump User manual

Brand
WPI
Model
SP101i Two Syringe Infusion Pump
Type
User manual
SP101i
Two-syringe infusion pump for microdialysis
INSTRUCTION MANUAL
Serial No._____________________
071607
World Precision Instruments
www.wpiinc.com
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 16
Copyright © 2007 by World Precision Instruments, Inc. All rights reserved. No part of this publication may be
reproduced or translated into any language, in any form, without prior written permission of World Precision
Instruments, Inc.
Contents
Introduction ...........................................................................................................1
Features ..................................................................................................................2
Keypad Functions ................................................................................................3
Display ....................................................................................................................4
Operating Instructions ........................................................................................4
Loading the Syringe ............................................................................................ 8
Manual Stall Setting and Microliter Syringes ...............................................9
NV RAM Failure .................................................................................................... 9
Power Selection ...................................................................................................9
Maintenance ......................................................................................................... 9
Specifications ......................................................................................................13
Warranty ..............................................................................................................14
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 1
Introduction
Operation of the pump is simplified by using a keypad to select features from
a menu displayed on an alphanumeric LCD. All control functions are performed
automatically by the pump microcontroller and are based on linear motion of the
pusher block, associated with the syringe size (diameter) to deliver a known volume.
After entering the syringe diameter, either directly or from a table in memory, all
calibration and control functions are performed by the pump automatically.
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 2
Features
The SP101i is an accurate single syringe infusion pump designed to hold glass or
plastic syringes, of any make, from 10 microliter to 60 milliliter.
Pump setup and operation is extremely simple. A menu, displayed on an
alphanumeric LCD “prompts” the operator to make the necessary selections using the
keypad for choice of features and numerical entries.
The internal diameter of the syringe is used by the control program to calibrate
the pump and deliver the volume and flow rate selected. For simplicity the syringe
diameter is also used to set volume and flow rate units.
The syringe diameter can be entered directly or the syringe can be identified from
a table of syringes held in memory. When the syringe is selected from the table the
diameter is entered automatically.
Two dispense modes are available:
a. dispense volume mode in which the pump keeps track of the volume
dispensed and automatically stops the pump when a set target volume is
reached .
b. run mode where the pump runs at the set flow rate until manually stopped .
In the event of a power interruption during operation, the pump can be
programmed to either resume operation or remain stopped when power is returned.
For convenience, all pump settings are stored in non-volatile memory to minimize
the number of setting changes required.
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 3
Keypad Functions
v This key has two functions:
a. Moves the display to the left.
b. Causes the numerical entry to decrease. To change the displayed
number by a single unit press and release the key quickly. If the key is
pressed longer the number changes with increasing speed.
n This key has two functions.
a. Moves the display to the right.
b. Causes the numerical entry to increase. To change the displayed
number by a single unit press and release the key quickly. If the key is
pressed longer the number changes with increasing speed.
Select This key has three functions:
a. Return to main menu when pressed repeatedly.
b. Selects the pulsing or “highlighted” feature of the main menu display.
c. Enter numerical values.
Run/stop This key has two functions:
a. Turns the motor on and off.
b. Acts as a “pause” during a dispense.
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 4
Display
After the pump is programmed an arrow on the right side of the display indicates the
direction of operation. This arrow pulses when the pump is running.
Operating Instructions
Power Switch
The power switch is located at the right, rear corner of the pump. When the power
is turned on the LCD will display the Volume or Rate setting. Press the select key to
return to the main menu.
Main Menu
Pressing select repeatedly will always return the display to the main menu. The main
menu consists of five variables, three of which are displayed at all times. The center
variable pulses to indicate that this option can be reviewed or changed. The menu
acts as a continuous loop and the arrow keys, vnare used to move around the
loop. The variable to be changed can be selected with the select key.
vn DIA vnTABLE vnRATE vnVOL vn PWR UP vn
direct entry syr dia. flow rate dispense vol run/stop
syr dia (mm) mfr, size µl or mL/h µl or mL
Syringe Diameter Entry
For automatic internal calibration the syringe diameter must be entered. Once
entered the diameter is retained in non-volatile memory and need not be entered
again unless the syringe used is changed. When a syringe diameter is changed the
Volume and Rate settings are set to zero.
There are two methods of diameter entry.
a. Internal library
Select TABLE from the main line menu. The table of syringes is organized by
manufacturer, then by glass or plastic, and then by size. Use the arrow keys
to scroll through the table and the select key to enter the correct setting. First,
scroll through the manufacturers and select the manufacturer of the syringe
used. The menu will next display either material selection or a syringe size.
Again, use the arrow keys to move through the table until the correct size
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 5
is displayed. Press the select key when the correct syringe size is displayed.
This automatically enters the internal syringe diameter.
See Table 1 at the end of this manual for the syringe library.
b. Direct entry
From the main menu select DIA. The display will read “xx.xx mm”. Use
the arrow keys to display the measured internal diameter of the syringe in
millimeters and enter with the select key.
Changing the diameter clears the Volume and Rate settings, and the display
will now prompt for the new Volume setting.
Volume Entry
The Volume setting mode is selected from the main menu or is displayed
automatically after changing the diameter.
Display reads: xx.xx µL (Units µL or mL depend on syringe diameter entered.)
1. Use the arrow keys to enter the dispense volume required.
2. When the correct dispense volume is displayed enter this number with the
select key.
Note: If no target dispense volume is required enter volume = 0.0 The pump will
run at the set flow rate until stopped manually or a stall occurs.
3. After setting a Volume the menu prompts for the flow rate setting.
When the pump runs the actual dispensed volume will be displayed and will
increment until the set volume is reached, at which time the pump will stop
automatically.
Flow Rate Entry
RATE can be selected from the main menu or will be displayed automatically after
the Volume setting.
The display reads: Rate: X.X µL/h or X.X mL/h
Rate units are µL/h or mL/h, microliter or milliliter/hour and are selected
automatically from the syringe diameter.
1. Use the arrow keys to display the flow rate required.
2. Enter the rate with the select key.
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 6
3. If the rate entered is out of range, a message is displayed “Max Rate XX
mL/h” To continue enter a lower rate or select a larger syringe.
If a dispense volume was set then the display will now change to “Volume :
0.0” , that is, in automatic dispense mode.
When the pump runs, the actual dispensed volume will be displayed and will
increment until the set volume is reached, at which time the pump will stop
automatically.
If no dispense volume is set the LCD displays the set flow rate and, when
running, the directional indicator arrow will pulse.
Starting the Pump
a. Press the run/stop key to start the pump. A second press will stop the pump.
b. If the pump is stopped during a dispense the volume accumulator is not
cleared and the dispense is paused. Restarting the pump with a second press
of the run/stop key continues the dispense to the target volume.
Power Failure Mode
When a dispense volume is set, a power interruption always stops the pump and
resets the volume dispense counter to zero. The pump remains stopped when power
is returned and displays either the Volume Setting (Pwr Stop mode) or “Power Failed”
flashing (Pwr Run mode). The pump can be restarted manually using the run or select
keys respectively.
When no dispense volume is selected, the Power Up run/stop option allows the pump
to either resume the dispense when the power returns or remain stopped but display
a message that the dispense was interrupted.
1. Move the main menu to the right and select PWR UP.
2. The display will read Power Up Run ( or STOP ). Use the arrow keys to change
display and select the required option.
RUN After power is returned the pump resumes normal operation. The display
flashes Power Failed to acknowledge a power interruption. Press Select to
display the Rate.
STOP Pump does not run when power returns. The LCD displays the rate setting;
the directional indicator does not pulse.
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 7
Change or Review Volume Setting While Running
While the pump is running it is possible to look at the volume and flow rate settings
without interrupting the operation of the pump. If required the volume and flow rate
settings can be changed while the pump continues to run. When the changes are
entered the pump immediately changes to the new settings.
1. While operating press select to return to the main menu.
2. Select Volume to display the set dispense volume. If no change is required
press select.
3. For a volume change the arrow keys can be used to change the setting which
is entered with the select key.
4. The display moves to RATE, permitting a change if required. Press select if
no change is required or use the arrow keys to change the setting. Pressing
select returns the display to the continuing volume dispense and the pump
immediately changes to the new flow rate, if changed. The volume continues
to increment, uninterrupted to the new dispense setting when it will stop
automatically.
Note: If the VOLUME is changed to a setting smaller than the volume already
accumulated then the pump stops when the new, smaller dispense volume is
selected.
Change or Review Rate Setting While Running
1. Press select to change the volume accumulator display to the main menu.
2. Select RATE.
3. Make rate changes if required and press select.
The pump immediately changes to the new flow rate and the display shows the
volume accumulation continuing, uninterrupted at the new rate.
Fast Forward
Press the run and the n keys simultaneously to actuate the fast forward mode.
The pump travels at its maximum rate while both keys are pressed. If one key is
deactuated the pump will stop and must be restarted with the run key.
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 8
Loading the Syringe
To facilitate loading, the pusher block (1) can be released from the leadscrew by
pressing the bronze button (2) and manually moving it along the guide rods to
accommodate the syringe.
Press the bronze button (2) on the side of the black pusher block (1) to release the
block from the leadscrew.
While keeping the button (2) pressed firmly “in” slide the block along the guide rods
to make way for the syringe plunger.
Raise the spring loaded retaining clamp (3) and rotate it away from the syringe barrel.
Place the syringe barrel in the V of the syringe holder, making sure that the flange of
the syringe barrel is pressed against the side of the syringe holder.
Rotate and release the syringe clamp to hold the syringe in place.
Press in button (2) and move the pusher block back along the guide rods to make
contact with the syringe plunger.
Pusher Block (1)
Release Button (2)
Syringe Plunger, Flange Syringe Clamp (3)
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 9
Manual Stall Setting and Microliter
Syringes
A movable collar located on the rear guide rod can be set to restrict travel of the
pusher block. The block moves until stalling against the collar. Stalling does no
permanent damage but may result in increased wear on the drive mechanism and
should be used only as a “fail-safe” device.
Microliter syringes with fine wire plungers can be damaged if the plunger is
forced into the end of the syringe barrel; the collar can be adjusted to prevent this
occurrence.
NV RAM Failure
If the settings in the non-volatile memory become corrupted the display will read
“NV RAM Failure” and the pump will not operate.
To recover from this condition the pump must be powered down and then turned
on again. The pump will be re-initialized to the default settings and can now be
programmed as normal.
Power Selection
The pump is equipped with an internal voltage selector switch which is set at the
factory to the voltage appropriate for the destination country. The voltage setting is
indicated on the serial number label found on the rear panel.
FUSE 5x20mm, 250V 0.1A (slow-blow)
Maintenance
Maintenance is required only for the moving mechanical parts which should be kept
clean and lubricated. Occasionally, a little light machine oil should be applied to the
guide rods and a little grease or oil to the leadscrew.
Surface cleaning may be performed on the pump by using a dampened cloth, taking
care that no excess cleaner be allowed to seep into the interior of the pump housing.
Do not use organic solvents.
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 10
Table 1 Table of syringe diameters
Air-Tite “All Plastic” Ranfac
1cc 4.70 mm 2 cc 9.12 mm
2.5 9.70 5 12.34
5.0 12.48 10 14.55
10 15.89 20 19.86
20 20.00 30 23.20
30 22.50 50 27.60
50 28.90
Becton Dickinson Scientific Glass Engineering
Interim, WW design, Plastipak SGE
1 cc 4.70 mm 25 µL 0.73 mm
3 8.59 50 1.03
5 11.99 100 1.46
10 14.48 250 2.30
20 19.05 500 3.26
30 21.59
60 26.60 1 mL 4.61mm
2.5 7.28
5 10.30
Becton Dickson 10 14.57
Glass — all types
0.5 cc 4.64 mm Sherwood - Monojet Plastic
1 4.64
2.5 8.66 1 cc 4.65 mm
5 11.86 3 0.94
10 14.34 6 12.70
20 19.13 12 15.90
30 22.70 20 20.40
60 28.60 35 23.80
50 26.60
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 11
Hamilton Terumo
1000-Series Gastight
10 µL 0.46 mm 1 cc 4.73 mm
25 0.78 3 9.00
50 1.03 5 13.04
100 1.46 10 15.79
250 2.30 20 20.18
500 3.26 30 23.36
1 mL 4.61 mm 60 29.45
2.5 7.28
5 10.30
10 14.57 Unimetrics
25 23.03 Series 9000
50 32.57
10 µL 0.46 mm
25 0.73
50 1.03
Popper & Sons, Inc. 100 1.46
Perfektum glass 250 2.30
500 3.26
0.25 3.45 mm 1000 4.61
0.5 3.45
1 4.50
2 8.92
3 8.99
5 11.70
10 14.70
20 19.58
30 22.70
50 29.00
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 12
Table 2 Flow Rates
Syringe Diameter Minimum Maximum
10 µL 0.46 mm 0.001 µL/min 0.350 µL/min
25 µL 0.73 mm 0.001 µL/min 0.884 µL/min
50 µL 1.03 mm 0.001 µL/min 1.759 µL/min
100 µL 1.46 mm 0.001 µL/min 3.526 µL/min
250 µL 2.3 mm 0.01 µL/min 8.78 µL/min
500 µL 3.26 mm 0.01 µL/min 17.65 µL/min
1 mL 4.61 mm 0.1 µL/min 35.2 µL/min
2.5 mL 7.28 mm 0.1 µL/min 88.0 µL/min
3 mL 8.59 mm 0.1 µL/min 122.5 µL/min
5 mL 10.3 mm 0.1 µL/min 176.2 µL/min
10 mL 14.57 mm 0.001 µL/min 0.351 mL/min
20 mL 19.05 mm 0.001 µL/min 0.602 mL/min
30 mL 21.59 mm 0.001 µL/min 0.773 mL/min
50 mL 28.9 mm 0.001 µL/min 1.387 mL/min
60 mL 26.6 mm 0.001 µL/min 1.175 mL/min
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 13
Specifications
Syringe Size 10 µL to 10 mL (two), or 60 mL (one)
Voltage Operating Range 95-130, 220-260 VAC
Drive Mechanism microprocessor controlled stepper motor 1/2
microstepping, driving a leadscrew through a
belt and pulley drive mechanism
Pusher Advance per Half Step 0.66 micron
Minimum Stepping Rate 1 step/30 sec
Maximum Stepping Rate 400 half-steps/sec
Speed Range 6000:1
Flow Rate Range 0.001 µL/min (10 µL syringe) to 1.175 mL/min
(60 mL syringe)
Linear Force 40 Ib (18 kg)
Power 115/230 VAC, 60/50 Hz, internal selector switch
Fuse 5x20 mm, 250 V, 0.1 amp (slow-blow)
Dimensions 9 x 6 x 5 in. (23 x 15.25 x 13 cm)
Weight 5 lb (2 kg)
SP101i Syringe Pump
WORLD PRECISION INSTRUMENTS 14
Warranty
WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment, including its
components and parts, shall be free from defects in material and workmanship for a period of one year* from the
date of receipt. WPI’s obligation under this warranty shall be limited to repair or replacement, at WPI’s option, of
the equipment or defective components or parts upon receipt thereof f.o.b. WPI, Sarasota, Florida U.S.A. Return of
a repaired instrument shall be f.o.b. Sarasota.
The above warranty is contingent upon normal usage and does not cover products which have been modified
without WPI’s approval or which have been subjected to unusual physical or electrical stress or on which the
original identification marks have been removed or altered. The above warranty will not apply if adjustment,
repair or parts replacement is required because of accident, neglect, misuse, failure of electric power, air
conditioning, humidity control, or causes other than normal and ordinary usage.
To the extent that any of its equipment is furnished by a manufacturer other than WPI, the foregoing warranty
shall be applicable only to the extent of the warranty furnished by such other manufacturer. This warranty will not
apply to appearance terms, such as knobs, handles, dials or the like.
WPI makes no warranty of any kind, express or implied or statutory, including without limitation any warranties
of merchantability and/or fitness for a particular purpose. WPI shall not be liable for any damages, whether direct,
indirect, special or consequential arising from a failure of this product to operate in the manner desired by the
user. WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use
of this product.
Claims and Returns
• Inspect all shipments upon receipt. Missing cartons or obvious damage to cartons should be noted on the
delivery receipt before signing. Concealed loss or damage should be reported at once to the carrier and an
inspection requested. All claims for shortage or damage must be made within 10 days after receipt of shipment.
Claims for lost shipments must be made within 30 days of invoice or other notification of shipment. Please save
damaged or pilfered cartons until claim settles. In some instances, photographic documentation may be required.
Some items are time sensitive; WPI assumes no extended warranty or any liability for use beyond the date
specified on the container.
• WPI cannot be held responsible for items damaged in shipment en route to us. Please enclose merchandise in
its original shipping container to avoid damage from handling. We recommend that you insure merchandise
when shipping. The customer is responsible for paying shipping expenses including adequate insurance on all
items returned.
• Do not return any goods to WPI without obtaining prior approval and instructions (RMA#) from our returns
department. Goods returned unauthorized or by collect freight may be refused. The RMA# must be clearly
displayed on the outside of the box, or the package will not be accepted. Please contact the RMA department for
a request form.
• Goods returned for repair must be reasonably clean and free of hazardous materials.
• A handling fee is charged for goods returned for exchange or credit. This fee may add up to 25% of the sale
price depending on the condition of the item. Goods ordered in error are also subject to the handling fee.
• Equipment which was built as a special order cannot be returned.
• Always refer to the RMA# when contacting WPI to obtain a status of your returned item.
• For any other issues regarding a claim or return, please contact the RMA department
Warning: This equipment is not designed or intended for use on humans.
* Electrodes, batteries
and other consumable
parts are warranted
for 30 days only from
the date on which the
customer receives these
items.
World Precision Instruments, Inc.
International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258
Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com
UK: Astonbury Farm Business Centre • Aston, Stevenage, Hertfordshire SG2 7EG • Tel: 01438-880025 • Fax: 01438-880026 • E-mail: [email protected]
Germany: Liegnitzer Str. 15, D-10999 Berlin • Tel: 030-6188845 • Fax: 030-6188670 • E-mail: wpide@wpi-europe.com
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WPI SP101i Two Syringe Infusion Pump User manual

Brand
WPI
Model
SP101i Two Syringe Infusion Pump
Type
User manual
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