Phcbi MDF-U5412H-PE Operating instructions

Please read the operating instructions carefully before using this product, and keep the operating

instructions for future use.

See page 42 for model number.

Operating Instructions

Biomedical Freezer

2

CONTENTS

INTRODUCTION P. 3

PRECAUTIONS FOR SAFE OPERATION P. 4

LABELS ON THE UNIT P. 9

SYMBOLS ON THE UNIT P. 10

ENVIRONMENTAL CONDITIONS P. 11

INTENDED USE AND PRECAUTIONS P. 11

FREEZER COMPONENTS

Main body P. 12

Control panel P. 14

INSTALLATION SITE P. 16

INSTALLATION P. 17

START-UP PROCEDURE P. 19

SETTING OF CHAMBER TEMPERATURE P. 20

LOCK OF CHAMBER TEMPERATURE SETTING P. 21

SETTING OF ALARM TEMPERATURE

Setting of high temperature alarm P. 22

Setting of low temperature alarm P. 23

SETTING OF DOOR ALARM DELAY P. 24

SETTING OF BUZZER SUSPENDED PERIOD P. 25

SETTING OF COMPRESSOR START DELAY P. 26

DURING/AFTER POWER FAILURE P. 27

REMOTE ALARM TERMINAL P. 28

ALARM FUNCTIONS P. 29

SAFETY FUNCTIONS P. 29

SELF DIAGNOSTIC FUNCTIONS P. 30

ROUTINE MAINTENANCE

Cleaning of exterior, interior, and accessories P. 31

Checking of alarm operation P. 31

Defrosting P. 32

CALIBRATION P. 32

REPLACEMENT OF BATTERY P. 33

TROUBLESHOOTING P. 34

DISPOSAL OF UNIT P. 35

Recycle of battery P. 35

Decontamination of unit P. 35

TEMPERATURE RECORDER (OPTION) P. 40

INTERFACE BOARD (OPTION) P. 40

SPECIFICATIONS P. 41

PERFORMANCE P. 42

SAFETY CHECK SHEET P. 43

3

INTRODUCTION

■ Read the operating instructions carefully before using the product and follow the instructions for safe

operation.

■ PHC Corporation takes no responsibility for safety if the product is not used as intended or is used with

any procedures other than those given in the operating instructions.

■ Keep the operating instructions in a suitable place so that they can be referred to as necessary.

■ The operating instructions are subject to change without notice for improvement of performance or

function.

■ Contact our sales representative or agent if any page of the operating instructions is lost or the page

order is incorrect, or if the instructions are unclear or inaccurate.

■ No part of the operating instructions may be reproduced in any form without the express written

permission of PHC Corporation.

IMPORTANT NOTICE

PHC Corporation guarantees this product under certain warranty conditions. However, please note that

PHC Corporation shall not be responsible for any loss or damage to the contents of the product.

4

PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with the operating instructions

as it contains important safety advice.

Items and procedures are described so that you can use this unit correctly and safely.

If the precautions advised are followed, this will prevent possible injury to the user and

any other person.

Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel

possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and

damage to the unit and associated property.

Symbol shows;

This symbol means caution.

This symbol means an action is prohibited.

This symbol means an instruction must be followed.

Be sure to keep the operating instructions in a place accessible to users of this unit.

5

PRECAUTIONS FOR SAFE OPERATION

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from

turning over. If the floor is not strong enough or the installation site is not adequate, this may result

in injury from the unit falling or tipping over.

Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping.

Improper installation may result in water spillage or injury from the unit tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location. This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present. The current leakage or

electric shock may result due to corrosion.

Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply

outlet is not grounded, it is necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod.

Inadequate grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed.

These may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use

may cause bad effect on your health or environment.

Turn off the power switch and disconnect the power supply to the unit prior to any

maintenance. Failure to follow this may cause electric shock or injury.

WARNING

6

PRECAUTIONS FOR SAFE OPERATION

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet

hand. This may cause electric shock.

Ensure you do not inhale or consume medication or aerosols from around the unit at the time

of maintenance. These may be harmful to your health.

Never splash water directly onto the unit. Water or liquid may cause electric shock or short circuit.

Never put containers with liquid on the unit. Water or liquid may cause electric shock or short

circuit.

Never bind, process, or step on the power supply cord, or never damage or break the power

supply plug. A broken supply cord or plug may cause fire or electric shock.

Do not use the power supply cord if there is something wrong with it. A broken supply cord or

plug may cause fire or electric shock.

Never disassemble, repair, or modify the unit or optional components yourself.. Any such work

carried out by an unauthorized person may result in fire, or electric shock or injury due to a

malfunction.

Disconnect the power supply plug if there is something wrong with the unit. Continued

abnormal operation may cause electric shock or fire.

When removing the power supply plug from the power supply outlet, grip the power supply

plug, not the cord. Pulling the cord may result in electric shock or fire by short circuit.

Before moving the unit, be absolutely sure to disconnect the power supply plug from the

power outlet, and then move the unit while ensuring that its power supply cord is not damaged.

If the power supply cord is damaged, electric shock and/or a fire may result.

Disconnect the power plug when the unit is not used for long periods. Keeping the connection

may cause electric shock, current leakage, or fire due to the deterioration of insulation.

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that

children do not have access and that doors cannot be closed completely. Removing doors

prevents accidents such as suffocation.

The disposal of the unit should be accomplished by appropriate personnel. Leaving the unit in

an unsupervised area may cause accidents.

Do not put the packing plastic bag within reach of children. Leaving the packing plastic bag may

cause accidents such as suffocation.

WARNING

7

PRECAUTIONS FOR SAFE OPERATION

The replacement of the battery for power failure alarm should be executed by a qualified

engineer or a service personnel only. The replacement of the battery for power failure alarm

involves the risk of electric shock.

Install the unit in a well-ventilated (airy) location to prevent the accumulation of flammable

refrigerant. The flammable refrigerant may cause fire if it leaks.

Never damage the chamber wall or pipework in the chamber when removing the frost. The

flammable refrigerant may cause fire if it leaks.

Flammable and explosive product. The unit contains flammable refrigerant. Be sure to follow the

below instructions when servicing or recycling.

 Well ventilate the room to prevent refrigerant accumulation.

 Keep fire away when the refrigerant is contained in the product.

 Do not damage or break the pipework.

Do not place this unit in a location where it is difficult to disconnect the power supply plug.

Failure to disconnect the power supply plug may cause fire in the event of a problem or malfunction.

WARNING

8

PRECAUTIONS FOR SAFE OPERATION

This unit must be plugged into a dedicated circuit protected by branch circuit breaker.

Use a dedicated power source as indicated on the rating label attached to the unit. A

multiple-tap may cause fire resulting from abnormal heating.

Connect the power supply plug to the power source firmly after removing the dust on the plug.

A dusty plug or improper insertion may cause a heat or ignition.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

At the recovery from the power failure, check the unit starts the operation properly. Also

check there is no change on the setting.

When moving the unit, contact our sales representative or agent. The moving by a user may

cause damage or injury resulting from tipping over of the unit.

Do not use a brush, an acid, a thinner, a laundry soap, a powder detergent, boiling water for

cleaning. These cause damage of painted surface or failure of plastic and rubber components.

Prepare a safety check sheet when you request any repair or maintenance for the safety of

service personnel. Copy the “Safety check sheet” and hand it to a service personnel after filling the

sheet.

Our company guarantees the product under certain warranty conditions. Our company in no

way shall be responsible for any loss of content or damage of content.

CAUTION

9

LABELS ON THE UNIT

Users are advised to avoid accidents by carefully reading the warnings and cautions contained on

warning labels at key locations on the interior and exterior of the unit.

Possible

Danger

Warning/Caution Type

Location of Danger

Warning/Caution Label Description of Danger

Personal

injury

Electric shock

Inside the cover for

space for a temperature

recorder

This is a power supply joint of

the temperature recorder.

Do not touch the power supply

joint to avoid the electric

shock.

Personal

injury

Flammable and

explosive product

Exterior, Interior

Flammable refrigerant may

cause fire if it leaks.

Personal

injury

Sample

damage

Frostbite

Chamber temperature

rise

Interior

No gloves may cause frostbite.

Too much frost may cause

chamber temperature rise

resulting from incomplete door

close.

Sample

damage

Chamber temperature

rise

Interior

Do not use a knife or a sharp

object for defrosting.

The failure may be caused if a

pipe or product is damaged.

10

SYMBOLS ON THE UNIT

The symbols are attached to the unit. The following table describes the symbols.

This symbol is attached to covers that access high-voltage electrical components to

prevent electric shock. Only a qualified engineer or service personnel should be

allowed to open these covers.

This symbol indicates that caution is required. Refer to product documentation for

details.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

11

ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC61010-1):

■ Indoor use;

■ Altitude up to 2000 m;

■ Ambient temperature 5oC to 40oC;

■ Maximum relative humidity 80% for temperature up to 31oC decreasing linearly to 50% relative humidity

at 40oC;

■ Mains supply voltage fluctuations up to ±10% of the nominal voltage;

■ Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;

■ Temporary OVERVOLTAGES occurring on the mains supply;

■ Applicable pollution degree of the intended environment (POLUTION DEGREE 2 in most cases)

INTENDED USE AND PRECAUTIONS

This equipment is designed for low temperature storage of DNAs and plasma.

 The effective storage period depends on the sample condition and storage temperature. It is

necessary to determine the storage temperature and period suitable for the purpose.

 For the plasma, the lower storage temperature should be required for longer term storage. It is

recommended to store the plasma at -80oC or lower.

12

FREEZER COMPONENTS

Main body

2

1

5

4

3

5

6

12

7

9 10 11 8

13

FREEZER COMPONENTS

1. Control panel: The chamber temperature and alarms can be set through the keys on the control panel.

The temperature display and indicators are also provided on the control panel [page 14].

2. Lock: By using with the enclosed key, both the upper door and lower door are locked or unlocked.

3. Leveling foot: This is the bolt for setting the unit and adjusting the level on the right and the left. At

the installation, extend the bolt until the front casters are away from the floor to set the unit [page 17].

4. Space for temperature recorder: A temperature recorder (option) is mounted here and the chamber

temperature can be recorded automatically [page 40].

5. Storage container: This is made of styrol resin. Be careful not to damage the storage container by

the scraper when removing the frost.

 The bottom 2 large storage containers are used for collecting the water when defrosting [page 32].

Note:

Do not pull the storage container excessively. The storage container may fall down.

6. Door latch: Always use the door latch to keep the door firmly.

7. Fixture (back side): A fixture is attached to the rear of the frame. Fix the frame to the wall with this

fixture and rope or chain [page 17].

8. Space for optional component: An interface board (MTR-480) or a LAN interface board (MTR-L03) is

installed here [page 40].

9. Remote alarm terminal: A remote alarm device (marketed product) can be connected to this terminal.

The alarm is noticed to an operator in the remote area by the remote alarm when the unit is installed in an

unmanned location [page 28].

10. Battery switch: This is a power switch of the battery for power failure alarm. Always keep ON this

switch during freezer operation to notice the power failure alarm.

11. Power switch: This is a power switch of the unit (ON=“l”,OFF=“○”).

12. Access port: This port is used to pass the sensor or cable of measuring equipment, or sensor of

temperature recorder (option) to the chamber.

 Always replace the rubber caps and the insulation to the access port when no use or the rise of

chamber temperature or condensation outside the access port may be resulted.

14

FREEZER COMPONENTS

Control panel

1. Door check indicator (DOOR):

The red LED lamp is lit when the door is open.

The buzzer sounds 2 minutes (initial setting)

after lighting of door check indicator to inform the

door open [page 29].

2. Alarm indicator (ALARM):

The red LED lamp blinks during an alarm

condition [page 29].

3. Battery indicator (BATTERY):

The orange LED lamp is lit to notice the time for

battery replacement [page 30].

4. Temperature display:

Normally shows the current chamber temperature

and during an alarm condition, shows an error

code etc. [page 30].

5. Up arrow key ( ):

At “temperature display mode”; Depressing this

key for more than 5 seconds leads setting mode.

At “setting mode”; By depressing this key, the

figure to be inputted (blinked digit) on the

temperature display can be changed [page 20 to

page 26].

At the setting of “lock of chamber temperature

setting”: By depressing this key, ON/OFF of the

lock can be selected [page 21].

6. Scroll key ( ):

At “temperature display mode”; Depressing this

key for more than 5 seconds leads the setting of

“lock of chamber temperature setting” [page 21].

At “setting mode”; Depressing this key leads the

movement between the digits to be inputted

(blinked digit) on the temperature display [page 20

to page 26].

7. Set key (SET):

At “temperature display mode”; depressing this

key leads “chamber temperature setting mode”.

At “setting mode”; Depress this key to enter the

set value during the setting procedure [page 20 to

page 26].

“temperature display mode”: the status that the

current chamber temperature is displayed on the

temperature display

“setting mode”; the status that the input on the

temperature display is effective by depressing the

up arrow key for more than 5 seconds.

5

4 2 1 3

6 7

15

FREEZER COMPONENTS

8. Defrost key (DEF.):

At “temperature display mode”; depressing this

key for more than 5 seconds leads the stop of

freezer operation. Depressing this key again

leads resumption of freezer operation. For the

details, refer to page 32.

9. Buzzer stop key (BUZZER):

The buzzer is silenced by depressing this key

when the alarm indicator blinks and the buzzer

sounds.

The buzzer sounds again after 30 minutes

(initial setting) even if the buzzer is stopped by

depressing the buzzer stop key (BUZZER)

when the same alarm status continues.

The alarm indicator blinks and the buzzer

sounds when other alarm is occurred in spite of

buzzer suspended period.

10. Alarm test key (ALARM TEST):

This key is used to check the operation of

buzzer, alarm indicator and remote alarm

terminal [page 31].

“temperature display mode”: the status that

the current chamber temperature is displayed on

the temperature display

8 9 10

16

INSTALLATION SITE

To operate this unit properly and to obtain maximum performance, install the unit in a location with the

following conditions:

 A location not subjected to direct sunlight

Do not install the unit under direct sunlight. Installation in a location subjected to direct sunlight cannot

obtain the intended performance.

 A location with adequate ventilation

Leave at least 10 cm around the unit for ventilation. Poor ventilation will result in a reduction of the

performance and consequently the failure.

 A location away from heat generating sources

Avoid installing the unit near heat-emitting appliances such as a heater or a boiler etc. Heat can

decrease the intended performance of the unit.

 A location with little temperature change

Install the unit under stable ambient temperature. The allowable ambient temperature is between 5oC

and 30oC.

 A location with a sturdy and level floor

Always install the unit on a sturdy and level floor. The uneven floor or tilted installation may cause failure

or injury. Install the unit in stable condition to avoid the vibration or noise. Unstable condition may

cause vibration or noise.

WARNING

Install the unit on a sturdy floor. If the floor is not strong enough or the installation site is not

adequate, this may result in injury from the unit falling or tipping over.

Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping.

Improper installation may result in water spillage or injury from the unit tipping over.

 A location not prone to high humidity

Install the unit in the ambient of 80%R.H. or less humidity. Installation under high humidity may cause

current leakage or electric shock.

WARNING

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

 A location without flammable or corrosive gas

Never install the unit in a flammable or corrosive location. This may cause explosion or fire or may result

in the current leakage or electric shock by the corrosion of the electrical components.

17

INSTALLATION

When installing the unit, follow the steps below to secure the unit properly, and also be absolutely sure to

connect the unit to ground.

In addition, install a ground fault circuit breaker (on the unit’s power supply side) which is mandatory

under the applicable laws and regulations.

1. Preparation after unpackaging

Remove all the tapes used to secure the doors and interior parts, and leave the doors open for a short

while for ventilation.

If any surfaces of the outer cabinet are dirty, wipe off the dirt using a cloth moistened with a diluted

neutral dish-washing detergent.

Using an undiluted solution of a neutral dish-washing detergent may cause the unit’s plastic areas to

crack. Follow the directions of the neutral dish-washing detergent for the dilution details.

After removing the dirt using the diluted neutral dish-washing detergent, be absolutely sure to wipe the

surfaces with a cloth dipped in clean water to remove the traces of the neutral dish-washing detergent.

After this, be absolutely sure to wipe the surfaces with a dry cloth, allow the surfaces of the outer cabinet

to dry out, and then proceed with the installation.

Note:

Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion of

the cord coating.

2. Securing and leveling the unit using the leveling feet

Rotate the front leveling feet clockwise until the casters are

raised 5 to 10 mm from the surface of the floor. (Fig. 1)

When the casters are raised from the surface of the floor,

the unit is secured. If they are left touching the floor, the

unit may inadvertently move out of position when its doors

are opened or closed.

In addition, rotate the leveling feet slightly clockwise or

counterclockwise, and adjust them so that the unit is level

between the left and right.

3. Securing the unit using the fixture

Use the fixtures on the rear panel of the unit, and secure

the unit to a wall with a strong rope or chain available on

the market. (Fig. 2)

Fig. 1

Leveling foot

Fixture

Fig. 2

18

INSTALLATION

4. Preventing electric shock by connecting the unit to ground

When installing the unit, be absolutely sure to connect it to ground. Grounding is necessary to prevent

the electric shock which will arise when the electrical insulation has deteriorated.

WARNING

Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet

is not grounded, it is necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

19

START-UP PROCEDURE

To commence the operation (initially or after temporary power off due to the cleaning, maintenance, etc.),

follow the procedure below:

The operation is resumed automatically at the recovery from power failure with the setting before power

failure [page 27].

1. Connect the power supply cord to the dedicated power source with the chamber empty.

2. Turn on the power switch.

►At the power on, the alarm indicator blinks and the blinking chamber temperature is displayed. (This is

not a malfunction.)

The blink of alarm indicator and the chamber temperature will stop when the chamber temperature goes

down around the chamber set temperature [Initial setting (factory setting): -40oC].

3. Turn on the battery switch.

4. Set the desired chamber temperature [page 20].

The factory setting of chamber temperature is -40oC.

5. On the temperature display, check the chamber temperature reaches the set temperature.

Allow the chamber temperature to fall to the set temperature when the start-up after cleaning,

maintenance or moving.

6. Put the material in the chamber gradually.

Putting a large amount of material in the chamber at a time causes the temperature rise.

7. As needed, set the alarm temperature [page 22 to 23], lock of chamber temperature setting [page 21],

door alarm delay [page 24],buzzer suspended period [page 25], and compressor start delay [page 26].

20

SETTING OF CHAMBER TEMPERATURE

Set the chamber temperature as needed to keep the material at appropriate temperature for long period

of time.

 Setting range of chamber temperature: between -45oC and-18oC

 Initial setting (factory setting): -40oC

The setting range of chamber temperature is between -45oC and -18oC, however the guaranteed

chamber temperature with no load is -40oC when the ambient temperature is 30oC.

Example: Change the setting of chamber temperature to -25oC from -40oC

► Following is an example. Operate the key according to the desired chamber temperature.

Description of operation Key operated Indication after operation

1

Connect the power supply plug to

the dedicated outlet and turn on

the power switch.

(only when start-up of unit)

-----

The current chamber temperature

blinks.

The alarm indicator blinks.

2 Depress the set key. SET The current setting (-40) is displayed

and the second figure 4 blinks.

3 Depress the up arrow key and

scroll the figure to 2.

The display changes to -20 from

-40.

4 Depress the scroll key once.

The first figure 0 blinks.

5 Depress the up arrow key and

scroll the figure to 5.

The display changes to -25 from

-20.

6 Depress the set key. SET

The temperature is memorized and

the current chamber temperature

blinks.

The setting mode returns to the temperature display mode automatically when 90 seconds has passed

without any key operation (auto-return function). In this case, the setting is not accepted.

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