Thermo Fisher Scientific VetMAX™ PRRSV EU & NA 3.0 Kit Operating instructions

Brand: Thermo Fisher Scientific

Model: VetMAX™ PRRSV EU & NA 3.0 Kit

Category: Car kits

Type: Operating instructions

VetMAX™ PRRSV EU & NA 3.0 Kit

RNA puriﬁcation protocols for use with the kit (Cat. No. A57016)

Catalog NumberA57016

Pub.No. MAN0029145Rev. A.0

WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear,

clothing, and gloves. Safety Data Sheets (SDSs) are available from thermoﬁsher.com/support.

WARNING! POTENTIAL BIOHAZARD. Read the biological hazard safety information at this product's page at

thermoﬁsher.com. Wear appropriate protective eyewear, clothing, and gloves.

Species Matrices Test type

Porcine

• Serum

• Whole blood

• Semen

• Tissue

• Oral ﬂuid

• Processing ﬂuid

Individual or pooled[1]

[1] Pool up to five samples for serum, whole blood, or semen sample matrices.

■Purpose of this guide ....................................................................................... 2

■Sample selection .......................................................................................... 2

■Sample storage ........................................................................................... 2

■Required materials not supplied .............................................................................. 3

■Purify RNA using the MagMAX™ CORE Nucleic Acid Puriﬁcation Kit (automated method) ................................. 3

■Good laboratory practices for PCR and RT-PCR ................................................................ 11

AppendixAPuriﬁcation with the KingFisher™ Duo Prime or KingFisher™mL instrument

■Required materials not supplied ............................................................................. 12

■Puriﬁcation procedure ..................................................................................... 12

Appendix B Documentation and support

■Customer and technical support ............................................................................. 13

■Limited product warranty ................................................................................... 13

SUPPLEMENTAL INSTRUCTIONS

For Laboratory Use.

Purpose of this guide

This guide describes methods of RNA puriﬁcation from samples (serum, whole blood, semen, tissue, oral ﬂuid, and processing ﬂuid)

using the MagMAX™ CORE Nucleic Acid Puriﬁcation Kit. The methods have been validated and optimized for later use with the VetMAX™

PRRSV EU & NA 3.0 Kit (Cat.No.A57016).

Sample selection

Sample matrix Type of analysis Quantity required and sampling equipment

Serum or whole blood Individual or pooled 200µL

Semen Individual or pooled 300µL

Tissue Individual 20–30 mg

Oral ﬂuid N/A[1] 300µL

Processing ﬂuid N/A[1] 200µL

[1] For this sample matrix, a mix of different animals or samples is used.

Sample storage

Sample matrix Storage

Serum (in dry tube) Maintain the samples at 2°C to 8°C until use (up to 8 days).

After use or after 8 days, store samples below −16°C for up to 1 year, or below −70°C for long‑term storage.

Whole blood (in EDTA tube) Maintain the samples at 2°C to 8°C until use (up to 8 days).

After use or after 8 days, store samples below −16°C for up to 1 year, or below −70°C for long‑term storage.

Semen Store samples according to the type of semen to be tested:

• For fresh semen, maintain the samples at 2°C to 8°C until use (up to 8 days).

• For commercial semen, maintain the samples according to the supplier's recommendation.

Tissue After sampling, store tissue samples at 2°C to 8°C if the analysis is to be performed within 24 hours of sampling.

After use or after 24 hours, store samples below −16°C for use within 1 year, or below −70°C for long-term storage.

Oral ﬂuid Maintain the samples at 2°C to 8°C until use (up to 7 days).

After use or after 7 days, store samples below −16°C for up to 1 year, or below −70°C for long‑term storage.

Processing ﬂuid Maintain the samples at 2°C to 8°C until use (up to 8 days).

After use or after 8 days, store samples below −16°C for up to 1 year, or below −70°C for long‑term storage.

2 VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions

Required materials not supplied

Unless otherwise indicated, all materials are available through thermoﬁsher.com. "MLS" indicates that the material is available from

ﬁsherscientiﬁc.com or another major laboratory supplier.

Table1Materials required for all sample preparation methods

Item Source

Instrument and equipment

One of the following instruments:

• KingFisher™ Flex Puriﬁcation System

• MagMAX™ Express‑96 Magnetic Particle Processor

See page11 for other compatible instruments.

Contact your local sales oce.

Precision micropipettes (range of 1 µL to 1,000µL) with DNase/RNase‑free ﬁlter tips MLS

Laboratory mixer, vortex or equivalent MLS

Benchtop microcentrifuge capable of 15,000 × gMLS

Precision scale MLS

Consumables

1.5‑mL and 2‑mL DNase/RNase‑free microtubes MLS

Kits and reagents

MagMAX™ CORE Nucleic Acid Puriﬁcation Kit A32700 or A32702

5 – IPC PRRS From the VetMAX™ PRRSV EU & NA 3.0 Kit

Sterile water —

RNase/DNase‑free water (for negative control samples (NCS)) MLS

Table2Optional equipment

Item Source

Biotang Inc Microplate Shaker, or equivalent titer plate shaker (for mixing beads with samples; all workﬂows) Fisher Scientiﬁc™ 50-751-4965

Benchtop centrifuge with plate adaptors (for lysate preparation in plates; Complex Workﬂow) MLS

Table3Additional materials required for the Simple Workﬂow (tissue samples only)

Item Source

Bead mill homogenizer for bead-beating, one of the following, or equivalent:

• Fisherbrand™ Bead Mill 24 Homogenizer (recommended)

• Mixer Mill MM 400 (Verder 207450001)

• Fisher Scientiﬁc™ 15-340-163

• Fisher Scientiﬁc™ 08418241

PYREX™ Solid Glass Beads for Distillation Columns (3mm) Fisher Scientiﬁc™ 11-312-10A

Purify RNA using the MagMAX™ CORE Nucleic Acid Puriﬁcation Kit (automated method)

Follow this procedure if you are using these instruments:

• KingFisher™ Flex

• MagMAX™ Express-96

Follow AppendixA, “Puriﬁcation with the KingFisher™ Duo Prime or KingFisher™mL instrument” if you are using these instruments:

• KingFisher™ Duo Prime

• KingFisher™mL

Workﬂow

Follow the appropriate workﬂow based on your sample type.

•RNA puriﬁcation from serum, whole blood, tissue and processing ﬂuid (Simple Workﬂow) (page6)

VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions 3

•RNA puriﬁcation from oral ﬂuid (Complex Workﬂow) (page8)

•RNA puriﬁcation from semen (page10)

RNA puriﬁcation from serum, whole blood, tissue and processing ﬂuid (Simple

Workﬂow)

Set up the processing plates (page6)

Prepare Bead/PK Mix (page6)

Prepare Lysis/Binding/IPC Solution (page7)

Prepare the sample (page7)

Combine the sample with the Bead/PK Mix and Lysis/Binding/IPC Solution

(page7)

Process samples on the instrument (page8)

RNA puriﬁcation from oral ﬂuid (Complex Workﬂow)

Set up the processing plates (page8)

Prepare Bead/PK Mix (page8)

Prepare Lysis/IPC Solution (page9)

Prepare the clariﬁed lysate (page9)

Combine the clariﬁed lysate with the Bead/PK Mix and MagMAX™ CORE Binding

Solution (page9)

Process samples on the instrument (page9)

4 VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions

RNA puriﬁcation from semen

Set up the processing plates (page10)

Prepare PK Mix (page10)

Treat samples with the PK Mix (page10)

Combine the sample with the Binding/Bead/IPC Mix (page11)

Process samples on the instrument (page11)

Procedural guidelines

• Before use, invert bottles of solutions and buers to ensure thorough mixing.

• Mix samples with reagents using a plate shaker or by pipetting up and down.

Note: Do not use a plate shaker with the tube strips that are required by the KingFisher™mL instrument.

• To prevent cross-contamination:

– Cover the plate or tube strip during the incubation and shaking steps, to prevent spill-over.

– Carefully pipet reagents and samples, to avoid splashing.

• To prevent nuclease contamination:

– Wear laboratory gloves during the procedures. Gloves protect you from the reagents, and they protect the nucleic acid from

nucleases that are present on skin.

– Use nucleic acid-free pipette tips to handle the reagents, and avoid putting used tips into the reagent containers.

– Decontaminate lab benches and pipettes before you begin.

Before ﬁrst use of the kit

(Optional) Determine the optimal bead mill homogenizer settings

We recommend using the Fisherbrand™ Bead Mill 24 Homogenizer for maximum nucleic acid yield. If an alternative instrument is used,

follow the manufacturer's guidelines to determine the speed and time settings necessary to achieve sucient cell lysis.

Determine the maximum plate shaker setting

If a plate shaker is used, use the following steps to determine the maximum setting.

1. Verify that the plate ﬁts securely on your shaker.

2. Add 1 mL of water to each well of the plate, then cover with sealing foil.

3. Determine the maximum setting that you can use on your shaker without any of the water splashing onto the sealing foil.

Download and install the script

The appropriate script for the MagMAX™ CORE Nucleic Acid Puriﬁcation Kit must be installed on the instrument before ﬁrst use.

VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions 5

1. On the MagMAX™ CORE Nucleic Acid Puriﬁcation Kit product web page (at thermoﬁsher.com, search by catalogue number), scroll

to the Product Literature section.

2. Right‑click the appropriate ﬁle to download the latest version of the MagMAX_CORE script for your instrument.

Table4Recommended scripts

Instrument

Script name

Standard script Express script[1]

KingFisher™ Flex MagMAX_CORE_Flex.bdz MagMAX_CORE_Flex_Express.bdz

KingFisher™96

MagMAX™ Express-96 MagMAX_CORE_KF-96.bdz MMC_KF96_Express.kf2

KingFisher™ Duo Prime MagMAX_CORE_DUO.bdz MagMAX_CORE_DUO_Express.bdz

KingFisher™mL MagMAX_CORE_mL_no_heat.bdz MagMAX_CORE_mL_Express.bdz

[1] The Express scripts do not have heating steps.

If required by your laboratory, use one of the following scripts, which do not heat the liquid during the elution step.

Table5Alternate scripts without heated elution step

Instrument

Script name

Standard script Express script

KingFisher™ Flex MagMAX_CORE_Flex_no_heat.bdz MagMAX_CORE_Flex_Express.bdz

KingFisher™96

MagMAX™ Express-96

MagMAX_CORE_KF-96_no_heat.bdz MMC_KF96_Express.kf2

KingFisher™ Duo Prime MagMAX_CORE_DUO_no_heat.bdz MagMAX_CORE_DUO_Express.bdz

KingFisher™mL MagMAX_CORE_mL_no_heat.bdz MagMAX_CORE_mL_Express.bdz

3. See your instrument user guide or contact Technical Support for instructions for installing the script.

Purify RNA from serum, whole blood, tissue, and processing ﬂuid (Simple Workﬂow)

1. Set up the processing plates on the KingFisher™ Flex or MagMAX™ Express-96 instrument.

Plate ID Plate position[1] Plate type Reagent Volume per well

Wash Plate 1 2 Deep Well MagMAX™ CORE Wash Solution 1 500µL

Wash Plate 2 3 Deep Well MagMAX™ CORE Wash Solution 2 500µL

Elution 4 Standard MagMAX™ CORE Elution Buer 90µL

Tip Comb 5 Standard Place a tip comb in the plate.

[1] Position on the instrument.

Note: To set up processing plates or tube strips for the KingFisher™ Duo Prime or

KingFisher™mL instrument, see page11.

2. (Optional) To prevent evaporation and contamination, cover the prepared processing plates with

sealing foil until they are loaded into the instrument.

1Set up the processing

plates

We recommend that you prepare new Bead/PK Mix for each processing run. If necessary, you can

store the Bead/PK Mix at 4°C for up to 1 week.

2Prepare Bead/PK Mix

6 VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions

2Prepare Bead/PK

Mix (continued)

1. Vortex the MagMAX™ CORE Magnetic Beads thoroughly to ensure that the beads are fully

resuspended.

2. Combine the following components for the required number of samples plus 10% overage.

Component Volume per sample

MagMAX™ CORE Magnetic Beads 20µL

MagMAX™ CORE Proteinase K 10µL

Total Bead/PKMix 30µL

1. Combine the following components for the required number of samples plus 10% overage.

Component Volume per sample

MagMAX™ CORE Lysis Solution 350µL

MagMAX™ CORE Binding Solution 350µL

5 – IPC PRRS[1] 2µL

Total Lysis/Binding/IPC Solution 702µL

[1] Supplied with the VetMAX™ PRRSV EU & NA 3.0 Kit.

2. Mix by inverting the tube or bottle at least 10times.

(Optional) Store the Lysis/Binding/IPC Solution at room temperature for up to 24 hours.

3Prepare

Lysis/Binding/IPC

Solution

Prepare samples according to sample type.

Sample type Action

• Serum

• Whole blood

• Processing ﬂuid

Proceed with 200µL of sample.

Tissue a. Add the following components to a 2-mL tube:

• Tissue—20 to 30mg

• PBS (1X), pH 7.4—1mL

• PYREX™ Solid Glass Beads for Distillation Columns (3mm)—2beads

b. Disrupt (bead-beat) the samples.

• Fisherbrand™ Bead Mill 24 Homogenizer—6m/s for 45seconds

• Mixer Mill MM 400—30 Hz for 5 minutes

Note: If an alternative bead mill homogenizer is used, see “(Optional) Determine

the optimal bead mill homogenizer settings” on page5.

c. Centrifuge at 1,000×g for 1 minute.

d. Proceed with 100 μL of supernatant.

4Prepare the sample

1. Invert the tube of Bead/PK Mix several times to resuspend the beads, then add 30µL of the

Bead/PK Mix to the required wells in the plate or tube strip.

2. Transfer the appropriate volume of each prepared sample to a well with the Bead/PKMix.

Sample type Amount

Serum, whole blood, or processing ﬂuid 200µL of sample

Tissue 100µL of supernatant

5Combine the sample

with the Bead/PKMix

and Lysis/Binding/IPC

Solution

VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions 7

5Combine the

sample with the

Bead/PKMix and

Lysis/Binding/IPC

Solution (continued)

3. Mix the sample with the Bead/PK Mix for 2 minutes at room temperature according to your

mixing method.

•Using a plate shaker—Shake vigorously for 2 minutes (see “Determine the maximum plate

shaker setting” on page5).

•By pipetting—Pipet up and down several times, then incubate for 2 minutes at room

temperature. (For downstream processing on the KingFisher™mL instrument, you must mix

by pipetting.)

4. Add 700 µL of the Lysis/Binding/IPC Solution to each sample-containing well or tube.

5. Immediately proceed to process samples on the instrument (next section).

1. Select the appropriate script on the instrument (see “Download and install the script” on page5).

2. Start the run, then load the prepared plates or tube strips in the appropriate positions when

prompted by the instrument.

Store puriﬁed nucleic acid on ice for immediate use, at −20°C for up to 1month, or at −80°C for

long‑term storage.

6Process samples on

the instrument

Purify RNA from oral ﬂuid (Complex Workﬂow)

1. Set up the processing plates on the KingFisher™ Flex or MagMAX™ Express-96 instrument.

Plate ID Plate position[1] Plate type Reagent Volume per well

Wash Plate 1 2 Deep Well MagMAX™ CORE Wash Solution 1 500µL

Wash Plate 2 3 Deep Well MagMAX™ CORE Wash Solution 2 500µL

Elution 4 Standard MagMAX™ CORE Elution Buer 90µL

Tip Comb 5 Standard Place a tip comb in the plate.

[1] Position on the instrument.

Note: To set up processing plates or tube strips for the KingFisher™ Duo Prime or

KingFisher™mL instrument, see page11.

2. (Optional) To prevent evaporation and contamination, cover the prepared processing plates with

sealing foil until they are loaded into the instrument.

1Set up the processing

plates

We recommend that you prepare new Bead/PK Mix for each processing run. If necessary, you can

store the Bead/PK Mix at 4°C for up to 1 week.

1. Vortex the MagMAX™ CORE Magnetic Beads thoroughly to ensure that the beads are fully

resuspended.

2. Combine the following components for the required number of samples plus 10% overage.

Component Volume per sample

MagMAX™ CORE Magnetic Beads 20µL

MagMAX™ CORE Proteinase K 10µL

Total Bead/PKMix 30µL

2Prepare Bead/PK Mix

8 VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions

1. Combine the following components for the required number of samples plus 10% overage.

Component Volume per sample

MagMAX™ CORE Lysis Solution 450µL

5 – IPC PRRS[1] 2µL

Total Lysis/IPC Solution 452µL

[1] Supplied with the VetMAX™ PRRSV EU & NA 3.0 Kit.

2. Mix by inverting the tube or bottle at least 10times.

(Optional) Store the Lysis/IPC Solution at room temperature for up to 24 hours.

3Prepare Lysis/IPC

Solution

1. Brieﬂy mix the oral ﬂuid sample, then proceed with 300µL of sample.

2. Add the Lysis/IPC Solution, then clarify the lysate.

Processing

equipment Action

Processing in

tubes

a. For each sample, add 450 µL of the Lysis/IPC Solution to a new 2-mL tube.

b. Add 300 µL of sample to the Lysis/IPC Solution.

c. Vortex vigorously for 3 minutes.

d. Centrifuge at 15,000×g for 2 minutes.

e. Proceed with 600μL of supernatant (clariﬁed lysate). Be careful to avoid

disrupting the pellet.

Processing in

plates

a. For each sample, add 450 µL of the Lysis/IPC Solution to the appropriate wells

of a deep-well plate.

b. Add 300 µL of sample to the Lysis/IPC Solution.

c. Seal the plate with sealing foil.

d. Shake the plate at moderate speed for 5 minutes.

e. Centrifuge at 3,000×g for 5 minutes.

f. Proceed with 600μL of supernatant (clariﬁed lysate). Be careful to avoid

disrupting the pellet.

4Prepare the clariﬁed

lysate

1. Invert the tube of Bead/PK Mix several times to resuspend the beads, then add 30µL of the

Bead/PK Mix to the required wells in the plate or tube strip.

2. Transfer 600µL of each clariﬁed lysate (see “Prepare the clariﬁed lysate” on page9) to a well

with the Bead/PKMix.

3. Mix the sample with the Bead/PK Mix for 2 minutes at room temperature according to your

mixing method.

•Using a plate shaker—Shake vigorously for 2 minutes (see “Determine the maximum plate

shaker setting” on page5).

•By pipetting—Pipet up and down several times, then incubate for 2 minutes at room

temperature. (For downstream processing on the KingFisher™mL instrument, you must mix

by pipetting.)

4. Add 350µL of MagMAX™ CORE Binding Solution.

5. Immediately proceed to process samples on the instrument (next section).

5Combine the clariﬁed

lysate with the

Bead/PKMix and

MagMAX™ CORE

Binding Solution

1. Select the appropriate script on the instrument (see “Download and install the script” on page5).

2. Start the run, then load the prepared plates or tube strips in the appropriate positions when

prompted by the instrument.

6Process samples on

the instrument

VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions 9

6Process samples

on the instrument

(continued)

Store puriﬁed nucleic acid on ice for immediate use, at −20°C for up to 1month, or at −80°C for

long‑term storage.

Purify RNA from semen

1. Set up the processing plates on the KingFisher™ Flex or MagMAX™ Express-96 instrument.

Plate ID Plate position[1] Plate type Reagent Volume per well

Wash Plate 1 2 Deep Well MagMAX™ CORE Wash Solution 1 500µL

Wash Plate 2 3 Deep Well MagMAX™ CORE Wash Solution 2 500µL

Elution 4 Standard MagMAX™ CORE Elution Buer 90µL

Tip Comb 5 Standard Place a tip comb in the plate.

[1] Position on the instrument.

Note: To set up processing plates or tube strips for the KingFisher™ Duo Prime or

KingFisher™mL instrument, see page11.

2. (Optional) To prevent evaporation and contamination, cover the prepared processing plates with

sealing foil until they are loaded into the instrument.

1Set up the processing

plates

Note: Prepare PK Mix immediately before use.

1. Combine the following components (in the order indicated) for the required number of samples,

plus 10% overage (recommended).

Component Volume per sample

PBS (1X), pH 7.4 200μL

MagMAX™ CORE Proteinase K 10μL

Total PK Mix 210μL

2. Invert the tube several times to mix, then centrifuge brieﬂy to collect the contents.

2Prepare PK Mix

1. Add semen sample and 400µL of the MagMAX™ CORE Lysis Solution to a fresh 2‑mL

microcentrifuge tube.

Sample type Amount

Fresh or frozen semen 300µL of sample

Commercial semen Volume recommended by the supplier

2. Vortex vigorously for 1 minute.

3. Immediately proceed to the next section.

3Prepare the sample

Perform this procedure in single tubes to avoid contamination. Do not use plates.

1. Add 210 µL of the PK Mix directly to the lysate.

2. Vortex vigorously for 1 minute, then centrifuge brieﬂy to collect the contents.

3. Incubate the sample for 30 minutes at 70°C.

4. Centrifuge at 15,000×g for 2 minutes to collect the contents.

4Treat samples with the

PK Mix

10 VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions

1. Transfer 600μL of each sample lysate to the appropriate wells in the sample plate or tube strip.

2. Vortex the MagMAX™ CORE Magnetic Beads thoroughly to ensure that the beads are fully

resuspended.

3. Prepare the Binding/Bead/IPC Mix—Combine the following components for the required number

of samples, plus 10% overage (recommended).

Component Volume per sample

MagMAX™ CORE Binding Solution 400µL

MagMAX™ CORE Magnetic Beads 20μL

5 – IPC[1] 2μL

Total Binding/Bead/IPC Mix 422µL

[1] Included in the VetMAX™ PRRSV EU & NA 3.0 Kit.

4. Mix the Binding/Bead/IPC Mix by inversion until the solution is homogeneous, then add 420μL

of the Binding/Bead/IPC Mix to each sample.

5. Immediately proceed to process samples on the instrument (next section).

5Combine samples with

the Binding/ Bead/IPC

Mix

1. Select the appropriate script on the instrument (see “Download and install the script” on page5).

2. Start the run, then load the prepared plates or tube strips in the appropriate positions when

prompted by the instrument.

Store puriﬁed nucleic acid on ice for immediate use, at −20°C for up to 1month, or at −80°C for

long‑term storage.

6Process samples on

the instrument

Good laboratory practices for PCR and RT-PCR

• Wear clean gloves and a clean lab coat.

– Do not wear the same gloves and lab coat that you have previously used when handling ampliﬁed products or preparing

samples.

• Change gloves if you suspect that they are contaminated.

• Maintain separate areas and dedicated equipment and supplies for:

– Sample preparation and reaction setup.

–Ampliﬁcation and analysis of products.

• Do not bring ampliﬁed products into the reaction setup area.

• Open and close all sample tubes carefully. Avoid splashing or spraying samples.

• Keep reactions and components capped as much as possible.

• Use a positive-displacement pipettor or aerosol‑resistant barrier pipette tips.

• Clean lab benches and equipment periodically with 10%bleach solution or DNA decontamination solution.

VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions 11

AppendixAPuriﬁcation with the KingFisher™ Duo Prime or KingFisher™mL instrument

Required materials not supplied

Unless otherwise indicated, all materials are available through thermoﬁsher.com. "MLS" indicates that the material is available from

ﬁsherscientiﬁc.com or another major laboratory supplier.

Table6Materials required for processing on the KingFisher™ Duo Prime instrument

Item Source

KingFisher™ Duo Prime Puriﬁcation System 5400110

KingFisher™ combi pack for microtiter 96 deep-well plate (includes 8 plates, 8 tip combs, and 8 elution strips and caps) (for Duo Prime

only) 97003530

KingFisher™ elution strip for 12 pin magnet (for Duo Prime only) (40 pieces)[1] 97003520

KingFisher™ 12-tip comb, for 96 deep-well plate (for Duo Prime only) (50 pieces)[1] 97003500

KingFisher™ 96 deep-well plate, sterile (for Duo Prime, Flex and Presto)[1] 95040460

[1] Included in the KingFisher™ combi pack (Cat. No. 97003530).

Table7Materials required for processing on the KingFisher™mL instrument

Item Source

KingFisher™mLPuriﬁcation System 5400050

KingFisher™mL Tubes and tip combs (for 240 samples) 97002141

KingFisher™mL Tip comb (800 pieces) 97002111

KingFisher™mL Tube (20x45 pieces) 97002121

Puriﬁcation procedure

Note: When performing this procedure for processing on the KingFisher™mL instrument, mix samples by pipetting up and down. Do not

use a plate shaker with the large tube strips required by this instrument.

1. Follow the workﬂow for your sample type, starting with sample lysate preparation through combining the samples with beads and

lysis solution.

Note: Do not set up processing plates or tubes before preparing samples.

12 VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions

2. Add MagMAX™ CORE Wash Solutions and MagMAX™ CORE Elution Buer to the indicated positions, according to your instrument.

Load the Tip Comb and all of the plates or tube strips at the same time. The instrument does not prompt you to load items

individually.

Table8Plate setup: KingFisher™ Duo Prime instrument

Row ID Row in the plate Plate type Reagent Volume per well

Sample A Deep Well Sample lysate/bead mix Varies by sample

Wash 1 B MagMAX™ CORE Wash Solution 1 500µL

Wash 2 C MagMAX™ CORE Wash Solution 2 500µL

Elution[1] Separate tube strip[2] Elution strip MagMAX™ CORE Elution Buer 90µL

Tip Comb H Deep Well Place a tip comb in the plate.

[1] Ensure that the elution strip is placed in the correct direction in the elution block.

[2] Placed on the heating element.

Table9Tube strip setup: KingFisher™mL instrument

Position ID Tube strip position Tube Reagent Volume per well

Sample 1 Standard Sample lysate/bead mix Varies by sample

Wash 1 2 MagMAX™ CORE Wash Solution 1 500µL

Wash 2 3 MagMAX™ CORE Wash Solution 2 500µL

Elution 4 MagMAX™ CORE Elution Buer 90µL

Tip Comb N/A N/A Slide the tip comb into the tip comb holder.

3. Select the appropriate script on the instrument (see “Download and install the script” on page5).

4. Start the run, then load the prepared plates or tube strips in the appropriate positions when prompted by the instrument.

Store puriﬁed nucleic acid on ice for immediate use, at −20°C for up to 1month, or at −80°C for long‑term storage.

AppendixBDocumentation and support

Customer and technical support

Visit thermoﬁsher.com/support for the latest service and support information.

• Worldwide contact telephone numbers

• Product support information

– Product FAQs

– Software, patches, and updates

– Training for many applications and instruments

• Order and web support

• Product documentation

– User guides, manuals, and protocols

–Certiﬁcates of Analysis

– Safety Data Sheets (SDSs; also known as MSDSs)

Note: For SDSs for reagents and chemicals from other manufacturers, contact the manufacturer.

Limited product warranty

Life Technologies Corporation and/or its aliate(s) warrant their products as set forth in the Life Technologies' General Terms and

Conditions of Sale at www.thermoﬁsher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please

contact Life Technologies at www.thermoﬁsher.com/support.

VetMAX™ PRRSV EU & NA 3.0 Kit Supplemental Instructions 13

Laboratoire Service International (LSI) | 6 Allée des Ecureuils – Parc Tertiaire du Bois-Dieu | 69380 Lissieu – France

For descriptions of symbols on product labels or product documents, go to thermoﬁsher.com/symbols-deﬁnition.

Revision history:Pub.No.MAN0029145 A.0

Revision Date Description

A.0 13 September 2023 New document for VetMAX™ PRRSV EU & NA 3.0 Kit.

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,

PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all

applicable Limited Use Label Licenses.

thermoﬁsher.com/support|thermoﬁsher.com/askaquestion

thermoﬁsher.com

13 September 2023

Thermo Fisher Scientific VetMAX™ PRRSV EU & NA 3.0 Kit Operating instructions

Brand: Thermo Fisher Scientific

Model: VetMAX™ PRRSV EU & NA 3.0 Kit

Category: Car kits

Type: Operating instructions

Download PDF

report

Thermo Fisher Scientific VetMAX™ PRRSV EU & NA 3.0 Kit Operating instructions

Thermo Fisher Scientific VetMAX™ PRRSV EU & NA 3.0 Kit Operating instructions

Related papers