Nikon eclipse fn1 Instructions Manual

Microscope

ECLIPSE FN1

Instructions

M368E 05.10.NF.1

1

Introduction

Thank you for purchasing the Nikon product.

This instruction manual is written for the users of the Nikon Microscope ECLIPSE FN1. To ensure correct usage,

please read this manual carefully before operating the product.

• It is prohibited to reproduce or transmit this manual in part or whole without Nikon's expressed permission.

• The contents of this manual are subject to change without notice.

• Although every effort has been made to ensure the accuracy of this manual, if you note any points that are

unclear or incorrect, contact your nearest Nikon representative.

• Some of the products described in this manual may not be included in the set you have purchased.

• Also be sure to read the manuals for any other products that you are using with this system (the super

high-pressure mercury lamp power supply, high-intensity light source, and such).

WARNING and CAUTION Symbols Used in This Manual

Although Nikon products are designed to provide the utmost safety during use, incorrect usage or failure to follow the

safety instructions provided may cause personal injury or property damage. Damage or injury that may occur due to

mishandling is unwarranted. To ensure correct usage, read the instruction manual carefully and thoroughly before

using the product. Do not discard the manual; keep it handy for easy reference.

Safety instructions within this manual are accompanied by the following symbols to highlight their importance. For

your safety, always follow the instructions accompanying these symbols.

Symbol Meaning

Disregarding instructions accompanying this symbol may lead to serious injury or

death.

Disregarding instructions accompanying this symbol may lead to injury or property

damage.

WARNING

CAUTION

Introduction

2

Meaning of the symbol used on the product

The symbol appearing on the product indicates the need for caution at all times during use. Always refer to the

instruction manual and read the relevant instructions before manipulating any part to which the symbol has been

affixed.

Caution for heat

This symbol on the lamphouse of the Eclipse FN1 calls your attention on the following:

• The lamp and its surroundings (including the lamp house) become very hot during and

immediately after lighting.

• Do not touch the lamp and its surroundings during and immediately after lighting to prevent

the risk of burns.

• Make sure that the lamp and its surroundings are sufficiently cool before the lamp

replacement.

3

Safety Precautions

Take note of the following points to use this product and its system safely and correctly.

1 Intended product use

This microscope system is intended primarily for microscopy and photomicrography of specimens such as

sliced living cells using the dia illumination or the epi illumination.

2 Do not disassemble

Disassembling the microscope or the microscope system may result in electric shock or malfunctions.

Malfunctions and damage due to disassembling are unwarranted.

Never attempt to disassemble any part other than the parts described in this manual. If you experience

problems with the microscope or the microscope system, contact your nearest Nikon representative.

3 Read the instructions carefully

To ensure safety, carefully read this manual and the manuals provided with any other equipment used with

this microscope. In particular, observe all warnings and cautions given at the beginning of each manual.

To use this microscope system with an epi-fl attachment:

The mercury lamp (or xenon lamp) used for the epi-fl microscopy requires special handling. Make sure to

see the manual provided for either the super high-pressure mercury lamp power supply or the

high-intensity light source.

4 Cautions about the power cord

Make sure to use the specified power cord. Using a wrong power cord may result in malfunctions or fire.

The power supply for this system is classified as subject to Class I protection against electrical shock.

Make sure the power supply unit is connected to an appropriate ground terminal (protective earth

terminal).

Refer to Chapter 7, "Specifications," for the specified power cord.

To prevent electrical shock, always turn off the power switch (press it to the "O" position) on the power

supply unit before attaching or detaching the power cord.

5 Cautions about heat from the light source

The lamp and its surroundings (including the lamp house) become very hot during and immediately after

lighting.

• Do not touch the lamp and its surroundings during and immediately after lighting. They become very

hot and may cause burn injuries.

• Make sure that the lamp and its surroundings are sufficiently cool before the lamp replacement.

• Do not allow cloth, paper, or highly flammable volatile materials, such as gasoline, benzine, paint

thinner or alcohol, to come near the lamp house while the lamp is lit or within 30 minutes after

switching off the power. They become very hot and may cause fire or burn injuries.

WARNING

Safety Precautions

4

6 Mercury lamp or xenon lamp

(for an epi-fl attachment)

The mercury lamp (or xenon lamp) used for the epi-fl attachment requires special handling. To use the

system safely and correctly, read the warnings below and be aware of the dangers. Also carefully read

the manual for the super high-pressure mercury lamp power supply (or high-intensity light source) and

the manual (if provided) by the manufacturer of the lamp and follow their instructions.

Warnings about the mercury lamp and the xenon lamp

1) The mercury lamp (or the xenon lamp) radiates ultraviolet light that is harmful to the eyes and skin

when the lamp is turned on. Don't look at the lamp directly. Direct viewing of light from these lamps

may result in blindness.

2) Gas is sealed under very high pressure inside the lamp. The pressure increases when the lamp is on.

If the lamp is scratched, dirty, subjected to high external pressure or physical impact, or used beyond

its operational life, the sealed gas may escape or the lamp may burst. In such cases, there is a

possibility of inhaling the gas, injury by broken piece of the glass, or other accidents.

3) When the lamp is on, the lamp and its surroundings become extremely hot. Do not tough the lamp

and its surroundings to prevent a burn injury and do not place flammable materials near the lamp to

prevent a fire.

4) Use only the lamps specified in this manual. Using other than the specified type of lamp could

result in an accident, such as a burst.

Because safety is a top priority in the design of Nikon products, the hazards described above should not

pose any danger as long as you heed all of the warnings and cautions in the manuals and use the system

only for its intended purpose. However, the hazards described above could lead to an accident if you fail

to heed all of the warnings and cautions in the manuals, if you strike the system, or if you attempt to

disassemble the system. Therefore, always be sure to heed all of the warnings and cautions.

7 Turn off the lamp before changing the filter cubes

(for an epi-fl attachment)

Before changing the filter cubes, be sure to turn off the light source of the epi-fl attachment. If the lamp is

lit and the filter cubes are changed, dangerous ultraviolet rays may be radiated.

8 Place the D/UV slider into the optical path

(for the D-FL epi-fl attachment)

To perform the bright-field microscopy under the episcopic illumination, the dark-field microscopy under

the episcopic illumination, or the DIC microscopy under the episcopic illumination with the mercury lamp

house, place the D/UV slider of the D-ES EPI ND slider into the optical path. If the D/UV slider is removed

from the optical path, dangerous ultraviolet rays will be radiated and the eyes and skin may be injured.

9 Installation of the microscope

To prevent the microscope from toppling over, fix the microscope FN1 body securely on a baseplate or a

vibration isolator with bolts.

WARNING

Safety Precautions

5

1 Isolate this microscope and its system equipment from the power source during assembly,

connecting/disconnecting cords, lamp replacement, and maintenance

To prevent electric shock and/or malfunctions, always turn off the power switches for the system (press

them to the "O" positions) and unplug the power cords from the wall outlet before assembly, connecting or

disconnecting cords, lamp replacement, and cleaning the microscopy and the objectives.

2 Cautions when replacing lamps

To prevent burn injuries, wait at least 30 minutes after the lamp is turned off to give it sufficient time to cool

down when replacing lamps. And, to avoid electric shock or malfunctions, never attempt to replace lamps

without turning off the power switches for the system (press them to the "O" positions). And then, unplug

the power cords from the wall outlet.

Make sure the lamp house cover is securely fitted to the lamp house after replacing lamps. Never turn on the

lamp while the lamp house cover is open.

When you dispose of the replaced lamp, do not break it up. Instead, dispose of the used lamp as special

industrial waste or dispose of it according to the local regulations and rules.

3 Use the specified light source

Use the specified light source. Using a wrong light source may cause a malfunction or a fire.

• For the illumination with the optical fiber

Specified light source: Sumita Optical Glass Inc. Halogen light source LS-DWL-N

• For the illumination with the lamp house

Specified lamp house: Nikon FN-LH

Specified lamp: 12V 100W Long life halogen lamp (PHILIPS 7724i or ODRAM HLX 64623)

Specified power supply: Nikon TE2-PS100W

* In addition, when the lamp house is used for the illumination, a lamp house adapter must be

attached to connect the lamp house with the microscope FN1.

4 Do not spill water or a chemical agent onto the microscope system

Never allow water or a chemical agent to come into contact with the microscope system. And do not use

the microscope system in circumstances where the microscope system is splashed with water or a

chemical agent. Water or a chemical agent splashed onto the power supply or the power supply device for

the optical fiber light source may cause a short circuit, resulting in malfunction or abnormal overheat. If the

product is subject to contact with water or a chemical agent, turn off the power switches for the product

(press them to the "O" positions). Then unplug the power cords from the wall outlet. Then, wipe off the

water with a piece of dry cloth. If water or a chemical agent enters the product, do not use the product and

contact your nearest Nikon representative.

5 Do not place any object on the top of the microscope or its system

Do not place any object on the top of the microscope or its sytem. Do not cover them with a piece of cloth

or so on. The system temperature will rise, resulting in malfunctions.

CAUTION

Safety Precautions

6

6 Cautions in assembling, installing, and carrying the microscope system

• Take care to avoid pinching your finger or hand during the microscope system assembly and

installation.

• Scratches or fouling such as fingerprints on optical components (lenses, filters, and so on) will

degrade microscope images. Be careful to avoid scratches or direct contact with the lenses and filters

when assembling the microscope system.

• To carry the microscope system, first detach all parts. And, tilt the microscope body while supporting

it, then securely hold it by the base.

• Never grasp the focus knobs, eyepiece tube, sliding nosepiece, nosepiece up/down part, stage,

substage, or condenser when carrying the microscope system. This may cause the part to come off,

resulting in damage to the microscope system.

• Do not install the product in a locker or a cabinet.

7 Do not turn on the power supply and the power supply for the optical fiber light source when a

cover is placed over the product.

Do not turn on the power supply and the power supply for the optical fiber light source when a cover is

placed over the product. A cover on the product blocks the heat radiation and it may cause abnormal heat

or fire hazards.

8 Cautions about long and continuous observations

To relieve fatigue resulting from long observation sessions, limit continuous observations to one hour.

Take at least 10- to 15-minute breaks between observation sessions. And, take care of the layout of

devices to be used and adjust the height of your chair.

CAUTION

Notes on Handling the Microscope System

7

Notes on Handling the Microscope System

1 Handle with care

This microscope system is a precision optical instrument. Handle the microscope system with care to avoid

shock on impact.

In particular, objectives may loose accuracy when exposed to even a weak physical shock.

2 Weak electromagnetic waves

The power supply for this microscope system emits weak electromagnetic waves. There is a possibility that

some precision electronic devices are affected by the electromagnetic waves. To prevent bad influences,

locate such electronic equipment away from the microscope system. If a TV or radio reception is affected,

move the TV or radio set farther from the product.

3 Dirt on the lens

Scratches or fouling such as fingerprints on optical components (lenses, filters, and so on) will degrade

microscope images.

If these parts become dirty, clean them as described in Capter 6, "Care and Maintenance" at the end of this

manual.

4 Dirt on the lamp

Never touch the lamp with bare hands. Dirt or fingerprints on the lamp will result in uneven illumination and

reduce the service life of the lamp. Always wear gloves when handling lamps.

5 Installation location

This microscope system is a precision optical instrument. So, the usage or storage in an inappropriate

environment may result in malfunctions or poor performance. Consider the following factors when selecting

an installation location:

• Fix the microscope FN1 body securely on a baseplate or a vibration isolator with bolts.

• When the microscope is mounted on a microscope slider such as an XY mover, fix the microscope

body on the microscope slider securely with bolts or so on to prevent the microscope from toppling

over.

• The halogen lamp light source, the lamp house, and the optical fiber light source need to dissipate heat

when the lamp is lit. So, locate such heat sources at least 10 cm away from walls.

• Choose a location less exposed to hazards in the event of collisions, earthquakes, or other potential

disasters. Choose a sturdy vibration isolator, desk, or table for the base of the microscope system.

• Avoid bright locations where the product is exposed to direct sunlight or immediately under room lights.

If the ambient light is bright, the microscopy image will be affected.

• Choose a location that is free from considerable dust or dirt.

• To avoid splashes, do not use the microscope system near water.

• Make sure the ambient temperature is 0 to 40°C and humidity is 85% or less. Using or storing the

product in hot, humid locations may result in mold formation or condensation on lenses, performance

degradation, or malfunctions.

• Select a layout that allows easy removal of the power cords from the AC inlets on the power supply

and the optical fiber light source in the event of an emergency.

Notes on Handling the Microscope System

8

6 Focus knobs

• Never turn the focus knobs on the left and right sides of the product in opposite directions at the same

time. Doing so may damage the product.

• Turning the coarse focus knob past its farthest point will damage the product. Never use excessive

force when turning the knob.

7 Protect the ports (when the trinocular eyepiece tube or the variable magnification double port tube

is attached).

Always attach caps to any port not in use. When caps are provided with equipment, use them. If any port is

not covered with a cap, extraneous light and dust may come into the equipment.

8 Handling filters (for an epi-fl attachment)

• Interference filters (especially excitation light filters, which are exposed to strong light) deteriorate over

time. Replace the filters as necessary.

• Filter characteristics may alter if the filter is exposed to high humidity. To prevent changes or

degradation of filter characteristics, avoid using or storing the filters under conditions of high humidity or

high temperature and avoid subjecting the filters to rapid temperature changes. When a filter is not in

use, store it in a desiccator or hermetically sealed container with a drying agent.

• The filters attached in the nine types of filter cubes listed below have sharper wavelength characteristics

than standard filters. However, due to their sophisticated coatings, they must be handled with special

care. In particular, take care to avoid abrasion from cleaning. Follow the procedure described in Section

1, "Lens Cleaning," of Chapter 6, "Care and Maintenance."）

Single band filter cubes: DAPI, FITC, TxRed, GFP

Multi band filter cubes: F-R, F-T, D-F, D-F-R, D-F-T

9 Handling

the D-FLD dark-field illumination cube (for an epi-fl attachment)

There are two blades in front of the D-FLD dark-field illumination cube to prevent light from coming through.

Do not apply any pressure onto the blades to prevent malfunctions.

10 Contents of the package

1) The contents of the package are listed below. Check them.

• Microscope ECLIPSE FN1

Main body x1

Substage part x1

Eyepiece tube mount part x1

Nosepiece arm part x1

Four base auxiliary legs x4

Tools

Hexagonal screwdriver x1

Two hexagonal wrenches x2

Screws

Two condenser centering screws x2

Condenser clamp screw x1

Notes on Handling the Microscope System

9

Two clamp screws for the eyepiece tube mount part front side (long type) x2

Two clamp screws for the eyepiece tube mount part rear side (short type) x2

Sixteen clamp screws for base auxiliary legs x16

Sixteen washers for base auxiliary legs x16

• Sliding nosepiece x1

• LWD condenser

Main body x1

Two diaphragms for the diagonal illumination x2

• Polarizer turret

Main body x1

1/4 lambda plate positioning tool x1

• Polarizer for visible lights x1

• Polarizer for infrared rays x1

• Variable magnification double port tube x1

• Single objective holder x1

• LWD 16x water immersion type objective x1

• LWD 16x DIC slider x1

• Variable magnification turret x1

• Rectangular stage

Stage main part x1

Two auxiliary support columns x2

Two auxiliary plates x2

Knurled ring x1

Acrylic chamber x1

Chamber holder x1

Eight flat head screws to clamp auxiliary plates x1

• Lamp house adapter

Main body x1

Raising parts x2

• Precentered lamp house x1

• Magnescale

Main body x1

Display part x1

• AC adapter x1

2) Do not discard the packing case, as it may be needed for future transportation.

Contents

10

Contents

Introduction...................................................................................................................................... 1

WARNING and CAUTION Symbols Used in This Manual................................................... 1

Meaning of the symbol used on the product ....................................................................... 2

Safety Precautions........................................................................................................................... 3

WARNING.......................................................................................................................... 3

CAUTION........................................................................................................................... 5

Notes on Handling the Microscope System ........................................................................ 7

Chapter 1 Part Name .................................................................................................................. 12

Chapter 2 Microscopy ................................................................................................................. 14

Chapter 3 Operation of Each Part ............................................................................................... 17

1 Coarse Focus Knob and Fine Focus Knob.......................................................... 17

1.1 Focus Knob Rotation and the Objective Vertical Movement............................ 17

1.2 Adjustment for the Rotation Torque (rotation Resistance)

of the Coarse Focus Knob ............................................................................... 17

2 Sliding Nosepiece ............................................................................................... 18

2.1 Objective Vertical Movement Lever and the Objective Position....................... 18

2.2 Switching Objectives........................................................................................ 19

2.3 Parfocal Adjustment for the Objectives ............................................................ 20

2.4 Dipping Function for a Water Immersion Type Objective................................. 20

2.5 Centering the Objective ................................................................................... 20

2.6 Attaching/Detaching the DIC Slider ................................................................. 21

3 Single Objective Holder....................................................................................... 21

4 Eyepiece Tube .................................................................................................... 21

4.1 Selecting the Optical Path in the Eyepiece Tube

(T Eyepiece Tube, F Eyepiece Tube)............................................................... 21

4.2 Diopter Adjustment........................................................................................... 22

4.3 Interpupillary Distance Adjustment................................................................... 23

5 LWD Condenser.................................................................................................. 23

5.1 Focusing and Centering the LWD Condenser ................................................. 23

5.2 Adjusting the Condenser Aperture Diaphragm ................................................ 24

5.3 Attaching an Optical Element into the LWD Condenser Turret ........................ 25

5.4 Operation of the LWD Condenser for Each Microscopy .................................. 26

6 Field Diaphragm.................................................................................................. 27

7 Stage .................................................................................................................. 27

8 Water Immersion Operation (Water Immersion Type Objective Handling) ........... 28

9 Cleaning the Oil Immersion Type Objective After Use ......................................... 28

10 Variable Magnification Double Port Attachment................................................... 29

10.1 Port Selection................................................................................................... 30

10.2 Mounting Parts of the Ports ............................................................................. 30

10.3 Mounting a 2/3 Inch TV Camera ...................................................................... 30

10.4 Aligning the Optical Paths for the Front Port and the Rear Port ...................... 31

10.5 Parfocal Adjustment for the Front Port and the Rear Port ............................... 31

10.6 Selecting the Magnification for the Rear Port .................................................. 31

10.7 For the DIC Microscopy ................................................................................... 32

Contents

11

11 Polarizer Turret ................................................................................................... 32

11.1 Attaching Filters ............................................................................................... 33

11.2 For the DIC microscopy ................................................................................... 33

11.3 Contrast Adjustment......................................................................................... 35

Chapter 4 Assembly.................................................................................................................... 36

Chapter 5 Troubleshooting.......................................................................................................... 49

1 Viewing Problems and Control Problems ............................................................ 49

2 Electrical System Problems................................................................................. 52

Chapter 6 Care and Maintenance ............................................................................................... 53

Chapter 7 Specifications ............................................................................................................. 54

12

Part Name

1

If the microscope is not assembled yet, refer to Chapter 4, "Assembly" and assemble the microscope beforehand.

For details about assembling and handling the power supply and the light source, see the instruction manuals for

them.

1. Main part of the microscope

This photo depicts the ECLIPSE FN1 microscope configured with the epi-fl attachment, the variable magnification

double port attachment, and the ergonomic eyepiece tube.

* The product name for the variable magnification double port attachment is "Mag. Variable Double Port."

* The ultraviolet light shield is removed from the

microscope in the photo above. The following figure

shows the ultraviolet light shield.

Eyepiece

Eyepiece tube

Diopter adjustment

ring

Slide click release button

Sliding nosepiece

Objective vertical movement

lever

Condenser

Base auxiliary leg

Sliding nosepiece lever

Epi-fl attachmen

t

Condenser focus knob

Condenser centering screw

Optical path selector lever

Variable magnification

double port attachment *

Magnification selector dial

Rectangular stage

Chambe

r

Ultraviolet light shield

Chapter 1 Part Name

13

2

2. TE2-PS100W power supply

The bottom of the power supply becomes extremely hot while it is in use. Do not obstruct the

air vents on the sides of the power supply.

A

nalyzer selector lever

Rear side fine focus knob

Rear side coarse focus knob

Stage movement knob

for the Y-axis

Stage movement knob

for the X-axis

Front side fine focus knob

Front side coarse focus knob

Coarse focus torque adjustment ring

Objective

Field diaphragm control

WARNING

Focus adjustment ring for the rear port

Lamp house

Parfocal correction

knob

Brightness

control knob

Power indicator

This lamp is lit when power is on.

Power switch

Push it to the "I" side to turn on.

Push it to the "O" side to turn off.

A

C inlet

Connect the specified power cord

that meets the voltage provided.

Refer to Chapter 7, "Specification"

for the specified power cord.

OUTPUT connector

This connector is used fo

r

the lamp.

It is connected to the lamp

house through the lamp

cable.

Sanwa Electric

SNS-1603-RSFb

Pin 1: Output +

Pin 2: Output -

Pin 3: Not used

14

Microscopy

2

The general procedure for the microscopy with water immersion objective is described below.

For details, refer to Chapter 3, "Operation of Each Part."

1

Turn on the power supply or the optical fiber light source. (Push the switch to the "I" postion.)

2

Adjust the brightness with the brightness control knob on the power supply or on the optical fiber

light source.

3

Rotate the polarizer turret to locate the desired filter into the optical path.

Under the standard configuration, the following filters are attached to the polarizer turret.

Upper turret: NCB11

Lower turret: ND filters (ND2, ND4, and ND16)

4

Push in the sliding nosepiece to locate the near

side objective, the low magnification objective (4x

or 10x), into the optical path.

CAUTION:

Make sure to move the objective vertical

movement lever to the center position

when you change objectives between

the near side one and the far side one.

You cannot change objectives when the

objective vertical movement lever is

placed at the right or the left. If you

attempt to change objectives forcibly,

the microscopy may be damaged.

Refer to Section 2.2 in Chapter 3, "Selecting an

Objective."

5

Place the specimen on the stage.

6

Rotate the condenser focus knob to raise the

LWDcondenser. The LWD condenser tip must be

raised to the limit position where it does not touch

the specimen bottom.

7

Fully open the field diaphragm with the field

diaphragm control. And fully open the condenser

aperture diaphragm with the condenser aperture

diaphragm lever.

8

Move the stage to place the target part of the

specimen into the optical path.

Push in

Condenser

focus knob

Field diaphragm

control

Condenser aperture diaphragm lever

Chapter 2 Microscopy

15

2

9

When the trinocular eyepiece tube is attached,

push in the optical path selector lever and select

the optical path to make 100% light go into the

binocular part.

Refer to Section 4.1 in Chapter 3, "Selecting the

Optical Path in the Eyepiece Tube (T Eyepiece

Tube, F Eyepiece Tube)."

10

Focus on the specimen with the coarse focus

knob and the fine focus knob.

11

Adjust the diopters and the interpupillary distance.

Check for your both eyes.

Refer to Section 4.2, "Diopter Adjustment" and

4.3, "Interpupillary Distance Adjustment" in

Chapter 3.

12

Focus and center the LWD condenser.

Refer to Section 5.1 in Chapter 3, "Focusing and

Centering the Condenser."

13

Move the objective vertical movement lever to the

center to retract the low magnification objective into

the upper position.

14

Pull the sliding nosepiece to the front to locate the

water immersion type objective into the optical path.

CAUTION:

Make sure to move the objective vertical

movement lever to the center position

when you change objectives between the

near side one and the far side one.

You cannot change objectives when the

objective vertical movement lever is placed

at the right or the left. If you attempt to

change objectives forcibly, the microscopy

may be damaged.

Condenser

centering screw

Push in

Move the objective vertical

movement lever to the center.

A

djust the diopters.

A

djust the interpupillary distance.

Pull

Chapter 2 Microscopy

16

15

Move the objective vertical movement lever to the

left so that the objective tip touches the surface of

the water over the specimen.

16

To perform the parfocal correction, use the

parfocal correction knob on the sliding nosepiece.

With this focusing operation, a confocal position

for the two objectives can be attained.

17

Adjust the brightness with the brightness control

knob on the power supply or on the optical fiber

light source.

18

Stop down the field diaphragm with the field

diaphragm control so that it circumscribes the

viewfield.

Refer to Section 6 in Chapter 3, "Field

Diaphragm."

19

Stop down the condenser aperture diaphragm

with the condenser aperture diaphragm lever to

about 70 to 80% of the numerical aperture of the

objective.

20

Start the microscopy.

Parfocal

correction

knobs

Condenser aperture

diaphragm lever

Field

diaphragm

control

17

Operation of Each Part

3

1

Coarse Focus Knob and Fine Focus Knob

1.1

Focus Knob Rotation and the Objective Vertical Movement

The rear side fine focus knob is marked in 1

µ

m

increments.

The objective rises/falls approximately 0.1 mm per one

turn of the fine focus knob.

The objective rises/falls approximately 14 mm per one turn

of the coarse focus knob.

The vertical movable range (coarse/fine focus stroke) of

the objective is 15 mm. It can be risen 13 mm above from

the focal point and can be lowered 2 mm below from the

focal point.

Focus knob rotation and the

objective vertical movement

Never attempt either of the following actions, as they will

damage the microscope.

• Rotating the left and right focus knobs in opposite directions

at the same time.

• Rotating the coarse focus knob past the stopper.

1.2

Adjustment for the Rotation Torque (rotation Resistance) of the Coarse

Focus Knob

The torque of the coarse focus knob can be adjusted.

To increase the torque, rotate the torque adjustment ring

at the base of the rear side coarse focus knob in the

counterclockwise direction. To decrease the torque, rotate

the ring in the clockwise direction.

CAUTION:

If the torque is adjusted very heavy and the

coarse focus knob is rotated, the microscope

may be damaged.

Coarse torque adjustment ring

Decrease the

torque

Increase the

torque

Chapter 3 Operation of Each Part

2 Sliding Nosepiece

18

2

Sliding Nosepiece

The sliding nosepiece can be attached two objectives into the near side socket and the far side socket.

The objectives are changed by sliding the nosepiece to and from.

2.1

Objective Vertical Movement Lever and the Objective Position

You can move the objective upward and downward by

operating the objective vertical movement lever on the

sliding nosepiece from side to side. This operation is

independent of the focus knob operation.

This operation is useful for retracting the objectives to

switch objectives and for dipping the objective into the

water to perform the water immersion.

You can operate the lever only when the objective is

placed in the optical path.

The vertical movable range of the objective is up to 15 mm

above the focal point. With this movable range, you can

observe a specimen in a high petri dish.

The table below shows the relationship between the objective vertical movement lever position and the

objective position.

Lever position

Near side objective position

(The near side objective is located

in the optical path.)

Far side objective position

(The far side objective is located in

the optical path.)

Right Observation position

Center Retracted position Retracted position

Left Observation position

Sliding nosepiece

Objective vertical

movement lever

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Nikon eclipse fn1 Instructions Manual

Nikon eclipse fn1 Instructions Manual

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