Leica Copier CM3600 User manual

Brand: Leica

Model: Copier CM3600

Type: User manual

This manual is also suitable for

CM3600

Leica CM3600

Cryomacrotome

Instruction manual

Leica CM3600

English V6.0 08/2006

Always keep this manual near the instrument.

Read carefully prior to operating the instrument

Order No.: 0708 35101

Instruction manual V 6.0 - 08/2006

Published by:

Leica Microsystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Telephone: +49 62 24 143-0

Facsimile: +49 62 24 143-200

E-mail: hi[email protected]

Internet : http://www.histo-solutions.com

. These are determined only by the contract pro-

visions agreed between ourselves and our cus-

tomers.

Leica reserves the right to change technical

specifications as well as manufacturing pro-

cesses without prior notice. Only in this way is it

possible to continuously improve the technology

and manufacturing techniques used in our prod-

ucts.

This document is protected under copyright laws.

Any copyrights of this document are retained by

Leica Microsystems Nussloch GmbH.

ny reproduction of text and illustrations (or of any

parts thereof) by means of print, photocopy, mi-

crofiche, web cam or other methods – including

any electronic systems and media – requires ex-

press prior permission in writing by Leica

Microsystems Nussloch GmbH.

For the instrument serial number and year of

manufacture, please refer to the name plate at

the back of the instrument.

The information, numerical data, notes and value

judgments contained in this manual represent the

current state of scientific knowledge and state-

of-the-art technology as we understand it follow-

ing thorough investigation in this field. We are

under no obligation to update the present manual

periodically and on an ongoing basis according

to the latest technical developments, nor to pro-

vide our customers with additional copies, up-

dates etc. of this manual.

For erroneous statements, drawings, technical

illustrations etc. contained in this manual we ex-

clude liability as far as permissible according to

the national legal system applicable in each indi-

vidual case. In particular, no liability whatsoever

is accepted for any financial loss or consequen-

tial damage caused by or related to compliance

with statements or other information in this

manual..

Statements, drawings, illustrations and other in-

formation as regards contents or technical de-

tails of the present manual are not to be consid-

ered as warranted characteristics of our

products.

NOTE

Leica CM3600 – Cryomacrotome

EC Declaration of conformity

We herewith declare in exclusive responsibility that the

Leica CM 3600 – Cryomacrotome

was developed, designed and manufactured to conform with:

Council Directive 89/392/EEC, Appendix II A (Machines),

Council Directive 73/23/EEC (low voltage) and

Council Directive 89/336/EEC, Appendix I (Electromagnetic compatibility),

including their amendments.

The following harmonized standards were applied:

DIN EN 292,

DIN EN 378 Part 1, DIN EN 61010-1.

The following national standards, guidelines and specifications were applied: MedGV.

In addition, the following in-house standards were applied:

DIN EN 9001.

A complete set of technical documentation is available.

An instruction manual for subject product is available.

Leica Microsystems Nussloch GmbH

Postfach 1120

D-69222 Nussloch

Germany

December 13, 1995

. . . . . . . . . . . . . . . . . . . . . . . . . . . .

Dag Graupner

Managing Director

Instruction manual V 6.0 - 08/2006

Index

1. Important notes ................................................................................................................... 8

1.1 Symbols in the text and their meaning ............................................................................................................................ 8

1.2 Qualification of personnel .................................................................................................................................................. 8

1.3 Instrument type .................................................................................................................................................................... 8

1.4 Designated use ..................................................................................................................................................................... 9

1.5 Warranty .............................................................................................................................................................................. 10

1.6 Service information ........................................................................................................................................................... 10

1.7 Decommissioning and disposal ...................................................................................................................................... 10

2. Characteristics of the Leica CM3600 ............................................................................ 12

2.1 Layout of the CM3600 ........................................................................................................................................................ 12

2.2 Technical Data ................................................................................................................................................................... 14

2.2.1 Microtome type ...................................................................................................................................................... 14

2.2.2 Control unit ......................................................................................................................................................... 14

2.2.3 Dimensions and weight ........................................................................................................................................ 15

2.2.4 Electrical connections ......................................................................................................................................... 15

2.2.5 Refrigeration system - cooling chamber .......................................................................................................... 16

2.2.6 General parameters .............................................................................................................................................. 17

2.3 Instrument parts ................................................................................................................................................................. 17

2.4 Function ............................................................................................................................................................................... 17

3. Safety................................................................................................................................... 20

3.1 Safety instructions ............................................................................................................................................................. 20

3.2 Warnings ............................................................................................................................................................................. 20

3.3 Safety standards ................................................................................................................................................................ 21

3.4 Safety devices .................................................................................................................................................................... 21

3.4.1 Emergency stop switch ........................................................................................................................................ 21

3.4.2 Knee lever ......................................................................................................................................................... 22

3.4.3 Window ......................................................................................................................................................... 22

3.4.4 Cryochamber ......................................................................................................................................................... 22

3.5 Special safety instructions .............................................................................................................................................. 23

4. Site requirements .............................................................................................................26

4.1 Site requirements at place of installation .................................................................................................................... 26

4.2 Electrical connections ...................................................................................................................................................... 27

4.3 Other connections ............................................................................................................................................................. 27

5 Installation ......................................................................................................................... 28

5.1 Unpacking and installation .............................................................................................................................................. 28

5.2 Standard delivery ............................................................................................................................................................... 28

5.3 Port and switch panel ....................................................................................................................................................... 29

5.3.1 Port and switch functions.................................................................................................................................... 30

Leica CM3600 – Cryomacrotome

Index

5.3.2 Fuses ......................................................................................................................................................... 31

5.4 PC .......................................................................................................................................................................................... 33

6. Software ............................................................................................................................. 34

6.1 Start and log-in procedure .............................................................................................................................................. 34

6.2 Description of the window elements ............................................................................................................................. 35

6.2.1 Title bar ......................................................................................................................................................... 35

6.2.2 Tool bar ......................................................................................................................................................... 35

6.2.3 Work space ......................................................................................................................................................... 37

6.2.4 Information bar ...................................................................................................................................................... 37

6.3 Initialization ......................................................................................................................................................................... 38

6.4 Main window ...................................................................................................................................................................... 40

6.4.1 Chamber temperature .......................................................................................................................................... 41

6.4.2 Time 41

6.4.3 Automatic defrost cycle ....................................................................................................................................... 41

6.4.4 Automatic dehydration ......................................................................................................................................... 42

6.4.5 Knife movement ..................................................................................................................................................... 42

6.4.6 Extraction system .................................................................................................................................................. 45

6.4.7 Sectioning program .............................................................................................................................................. 45

6.4.8 Sledge speed 46

6.4.9 Mode of operation ................................................................................................................................................. 47

6.4.10 Cutting window ....................................................................................................................................................... 48

6.5 Parameter settings ............................................................................................................................................................ 49

6.5.1 Parameter setting .................................................................................................................................................. 50

6.5.2 Password list ......................................................................................................................................................... 54

6.5.3 Configuration ......................................................................................................................................................... 57

6.5.4 Reference voltages ............................................................................................................................................... 64

6.5.5 Language selection ............................................................................................................................................... 64

6.5.6 Initialization ......................................................................................................................................................... 64

6.6 Temperature curves .......................................................................................................................................................... 65

6.6.1 Work space ......................................................................................................................................................... 66

6.6.2 Diagram ......................................................................................................................................................... 68

6.7 Chronological event list .................................................................................................................................................... 69

6.7.1 Work space ......................................................................................................................................................... 70

6.7.2 Event table ...............................................................................................................

.......................................... 72

6.8 Alarm list .............................................................................................................................................................................. 73

6.8.1 Work space ......................................................................................................................................................... 74

6.8.2 Event table ......................................................................................................................................................... 75

6.8.3 Error codes: meaning and troubleshooting ..................................................................................................... 76

6.9 Chamber illumination ........................................................................................................................................................ 77

6.10 Section documentation .................................................................................................................................................... 77

6.10.1 Tree diagram ......................................................................................................................................................... 78

6.10.2 Studies protocol ..................................................................................................................................................... 79

6.10.3 Event table ......................................................................................................................................................... 82

6.11 Photo mode ......................................................................................................................................................................... 83

Instruction manual V 6.0 - 08/2006

Index

6.12 Screen printout................................................................................................................................................................... 83

6.13 Remote inquiry .................................................................................................................................................................... 84

6.14 Help ....................................................................................................................................................................................... 86

6.15 Saving ................................................................................................................................................................................... 86

6.16 Printing labels ..................................................................................................................................................................... 86

6.17 Foot switch .......................................................................................................................................................................... 86

6.18 User log-in / log-out ........................................................................................................................................................... 87

6.19 Quitting the application .................................................................................................................................................... 87

7. Operating the Leica CM3600 ........................................................................................... 88

7.1 Check list ............................................................................................................................................................................. 88

7.2 Switching the instrument on ........................................................................................................................................... 89

7.3 Starting the software ........................................................................................................................................................ 89

7.4 Setting the chamber temperature .................................................................................................................................. 89

7.5 Specimen stages................................................................................................................................................................ 90

7.5.1 Inserting the specimen stage, orientable, with ball-joint ............................................................................. 92

7.5.2 Inserting the specimen stage, non-orientable............................................................................................... 94

7.6 Knife ...................................................................................................................................................................................... 96

7.6.1 Inserting standard knives .................................................................................................................................... 98

7.6.2 Inserting the disposable blade holder with disposable blade................................................................... 100

7.6.3 Adjusting the knife angle ................................................................................................................................... 102

7.7 Setting the trimming parameters .................................................................................................................................. 103

7.8 Setting the sectioning parameters .............................................................................................................................. 104

7.8.1 Sectioning the specimen ................................................................................................................................... 104

7.8.2 Collecting the sections ....................................................................................................................................... 104

7.9 Dehydrating the sections ............................................................................................................................................... 106

8. Cleaning and maintenance ........................................................................................... 108

8.1 Cleaning ............................................................................................................................................................................. 108

8.1.2 Removing the microtome from the cryochamber ......................................................................................... 108

8.2 Maintenance ..................................................................................................................................................................... 110

8.2.1 Oiling ....................................................................................................................................................... 110

8.2.2 Defrosting ....................................................................................................................................................... 111

9. Optional accessories ..................................................................................................... 112

9.1 Extraction system ............................................................................................................................................................ 112

9.1.1 Design and function of the extraction system .............................................................................................. 112

9.1.2 Exchanging the disposable bag ....................................................................................................................... 114

9.2 Ordering information ....................................................................................................................................................... 115

Leica CM3600 – Cryomacrotome

Index

Instruction manual V 6.0 - 08/2006

1.1 Symbols in the text and their meaning

Notes, i.e. important user information

appear in a grey box and are marked

with an information symbol .

Buttons and function keys which need

to be pushed on the data entry screen,

are printed in bold capitals.

Figures in brackets refer to item num-

bers in drawings.

(5)

1.3 Instrument type

All information contained in this instruction

manual applies exclusively to the instrument type

indicated on the title page.

A name plate bearing the instrument serial num-

ber is attached to the back of the instrument.

1.2 Qualification of personnel

Only trained laboratory personnel may operate the

Leica CM3600.

Prior to operating the instrument, the operator

must thoroughly read and understand this instruc-

tion manual and must familiarize him/herself with

all technical details of the instrument.

Warnings appear in a grey box and

are marked with a warning triangle

1. Important notes

ENTER

Leica CM3600 – Cryomacrotome

1.4 Designated use

The Leica CM3600 is an SPS-controlled and motorized precision

cryomacrotome for large-surface sections, equipped with a convection-

cooled cryochamber for deep-freezing, sectioning, storing and freeze-

drying large biological and industrial specimens.

The instrument is designed for the above described applications only and

must be operated according to the instructions in the present instruction

manual.

Any other use of the instrument is considered improper.

If you wish to use the instrument for special applications or modes of

operation not listed in this manual, it is absolutely necessary that you

contact your local Leica representation prior to doing so.

Otherwise, you will loose the Leica warranty on your instrument.

Only trained personnel, skilled in the use of the above-mentioned applica-

tions, may operate the Leica CM3600.

Prior to operating the instrument, the operator must thoroughly read and

understand this instruction manual.

1. Important notes

Instruction manual V 6.0 - 08/2006

1.5 Warranty

Leica Microsystems Nussloch GmbH guarantees that the contractual prod-

uct delivered has been subjected to a comprehensive quality control proce-

dure based on the Leica in-house testing standards, and that the product is

faultless and complies with all technical specifications and/or agreed char-

acteristics warranted.

The scope of the warranty is based on the content of the concluded agree-

ment. The warranty terms of your Leica sales organization or the organiza-

tion from which you have purchased the contractual product shall apply

exclusively.

1.6 Service information

If you require technical service or replacement parts, please contact your

Leica sales representative or Leica dealer who sold the product..

Please provide the following information:

• Model name and serial number of the instrument

• Location of the instrument and name of the person to contact

• Reason for the service call

• Date of delivery

1.7 Decommissioning and disposal

The instrument or parts of the instrument must be disposed of in compliance

with the local laws.

1. Important notes

Leica CM3600 – Cryomacrotome

1. Important notes

Instruction manual V 6.0 - 08/2006

2.1 Layout of the CM3600

Knee lever

Window Chamber lidEmergency stop

switch

Port and switch panel

Chamber

Refrigeration

and control unit

Socket panel

Microtome

(in chamber)

Port and switch panel

Drain tap

2. Characteristics of the Leica CM3600

Leica CM3600 – Cryomacrotome

Drain tap

Condensation water is drained through the drain tap.

Refrigeration and control unit

The refrigeration and control unit cools the cryochamber and controls the

microtome and the entire electronics.

Knee lever

The knee lever is used for manual operation of the specimen sledge.

Socket panel

The PC and peripherals are connected via the socket panel.

Commercial PC, monitor, keyboard, mouse and internal modem.

Emergency stop switch

Press the emergency stop switch to stop the microtome immediately.

Cabinet

The cabinet contains the cryochamber which houses the microtome.

Chamber lid

The chamber lid can be opened completely for maintenance and cleaning

work on microtome and/or cryochamber.

Window

The window is heated and can be opened completely.

Microtome

The microtome is used to section the specimens.

Port and switch panel

The port and switch panel contains the mains switch and several sockets

and ports.

2. Characteristics of the Leica CM3600

Instruction manual V 6.0 - 08/2006

2.2 Technical Data

2.2.1 Microtome type

450 C Sliding microtome

Section thickness selection: 1 - 200 μm

Sectioning range: 0 - 450 mm

Max. sectioning speed: 80 mm/s

Knife retraction after sectioning stroke: 50 - 250 μm

Total vertical stroke of knife: 70 mm (standard)

Total vertical stroke of knife: 100 mm (model with

extended vertical

stroke)

Total horizontal stroke: 450 mm

Specimen orientation via ball-joint: ca. 5° (x/y/z axis)

Specimen orientation w/large spec. stage

(450 x 150 mm): Rotation around

longitudinal axis (x axis)

Maximum specimen size (L x W x H): 450 x 150 x 70 mm

(standard)

Maximum specimen size (L x W x H): 450 x 150 x 200 mm

(model with extended

stroke length)

Knife holder for steel and

Tungsten Carbide knives: 160 mm

Knife holder for disposable blade holder: 160 mm

Knife (160 mm) with facet angle of: 20°, 35°

2.2.2 Control unit

The Leica CM3600 is equipped with a commercial PC, monitor, keyboard,

mouse and an internal modem. For further information, please refer to the

manual of the PC.

Operating system: XP.

Software: CM3600 Version 3.0.0.

2. Characteristics of the Leica CM3600

Leica CM3600 – Cryomacrotome

2.2.3 Dimensions and weight

Inner (cryochamber L x H x W): 1650 x 720 x 600 mm

Outer (L x H x W): 2800 x 1090 x 920 mm

(Overall dimensions with

connecting cables)

Outer length (total): 2800 mm

Outer length (chamber only): 1940 mm

Outer length (control unit

with connections only): 860 mm

Outer height (total): 1090 mm

Outer height (work surface height): 1050 mm

Outer width: 920 mm

Weight (total, incl. microtome): 660 kgs

Weight (microtome only): 190 kgs

2.2.4 Electrical connections

380 V / 50 Hz, three-phase

Nominal voltage: 380 V AC ± 10 %

Nominal frequency: 50 Hz

Power draw: 2300 VA

Automatic cutout/ 3 x 8A / 1 x 16A,

mains safety switch according to DIN IEC 127-2

208 V / 60 Hz, two-phase

Nominal voltage: 208 V AC ± 10 %

Nominal frequency: 60 Hz

Power draw: 2300 VA

Automatic cutout/ 2 x 8A / 1 x 10A

Mains safety switch UL-listed

2. Characteristics of the Leica CM3600

Instruction manual V 6.0 - 08/2006

200 V / 50 Hz, two-phase

Nominal voltage: 200 V AC ± 10 %

Nominal frequency: 50 Hz

Power draw: 2300 VA

Automatic cutout/ 2 x 8A / 1 x 10A

Mains safety switch UL-listed

200 V / 60 Hz, two-phase

Nominal voltage: 200 V AC ± 10 %

Nominal frequency: 60 Hz

Power draw: 2300 VA

Automatic cutout/ 2 x 8A / 1 x 10A

Mains safety switch UL-listed

2.2.5 Refrigeration system - cooling chamber

Selectable temperature range: 0° C to -30° C

at ambient temperature of

22° C, with window closed

and relative humidity ≤ 60 %: -30° C ±10 % (selectable

in 1 K steps)

Control accuracy: +1 to -3 K

Electrical power draw: 1160 W

Refrigerating output at -25°C: 1370 W

Nominal pressure: 20 bar (maximum value)

Cutoff pressure: 25 bar (via Pressostat)

Safety factor: 2, as per UL 1262

Refrigerant: 1100 g, refrigerant R404A

Compressor oil: 1220 ccm alpha 22, Kyodo

Defrosting: automatic hot-gas defrosting, programmable,

2 x automatic hot-gas defrosting cycles / 24 hours.

Duration: 1 - 15 minutes, manual defrosting as

needed.

2. Characteristics of the Leica CM3600

Leica CM3600 – Cryomacrotome

2.2.6 General parameters

Protective class: I

Pollution degree: 2

Overvoltage installation category: II

2.3 Instrument parts

The Leica CM3600 consists of a sliding microtome in a large-volume

cryochamber. The instrument is designed for cryosectioning large speci-

mens or for processing specimens with the so-called cryo-planing

technique.

The large-scale heavy-duty microtome is made for sectioning large

biomedical and industrial specimens such as whole animals or large

tissue specimens by applying the sectioning techniques for large-surface

sections. The sturdy construction of the instrument also permits section-

ing very hard specimens such as undecalcified bones.

The microtome is housed in a convection-cooled large-volume

cryochamber.

The large chamber in connection with the special cooling system enables

dehydration of sections within a very short time.

The low temperatures are achieved and maintained by cold air circulating

inside the cryochamber. A fan controls the air circulation speed.

The large specimens are frozen onto metal specimen stages which are

subsequently clamped in the specimen sledge.

Optionally the instrument can be operated with an extraction system

which largely prevents the cryochamber being soiled with section waste.

2.4 Function

The motor-driven specimen sledge moves horizontally underneath the

knife, either producing a section (cryosectioning) with each stroke or a

high-quality specimen surface (cryo-planing technique).

2. Characteristics of the Leica CM3600

Instruction manual V 6.0 - 08/2006

Prior to each sectioning stroke, a stepper motor feeds the microtome

knife downwards towards the specimen by a selected amount of microns

(section thickness).

During each return stroke of the specimen sledge, the stepper motor

automatically carries out a knife retraction movement, programmable

between 50 and 250 μm. This protects both the knife edge and the speci-

men surface from being damaged.

Before starting the next sectioning stroke, the motor lowers the knife

towards the specimen surface by the selected sectioning feed plus the

set value for knife retraction.

Trimming is programmable; the desired section thickness can be

preselected.

The number of sections to be preformed during trimming can be pro-

grammed via the section counter. When the programmed number of

sections has been carried out, the microtome will stop automatically.

Once the desired specimen block height has been reached, those sec-

tions that will actually be used for further examination are collected

manually via the knee lever.

The Leica CM3600 is operated via PC and/or manually via the knee lever.

All data entries are made via the PC.

The Leica CM3600 features two automatic defrost cycles.

Defrost cycle duration is software-controlled. If the evaporator surface is

already completely deiced before the programmed defrost time is up, the

defrost cycle will be aborted. In addition, manual defrost cycles can be

set as needed.

2. Characteristics of the Leica CM3600

Leica CM3600 – Cryomacrotome

2. Characteristics of the Leica CM3600

Instruction manual V 6.0 - 08/2006

3. Safety

3.1 Safety instructions

This instruction manual contains important in-

structions and information regarding the opera-

tional safety and maintenance of the instrument.

The instruction manual is an important part of the

product, which must be read carefully prior to

setup and use and must always be kept near the

instrument.

This Instruction manual must be ap-

propriately supplemented as re-

quired by the existing regulations

on accident prevention and environ-

mental safety in the operator‘s

country.

Be sure to comply with the safety instructions and warnings in this chapter.

Read these instructions, even if you are already familiar with the operation and use of

other Leica products.

The Leica CM3600 was built and tested in accor-

dance with the safety regulations for electrical

measuring, control, regulating and laboratory

devices listed below:

• DIN EN ISO 9001,

• EN 61010-1, EN 61010-2-010,

• EN 292-1, EN 292-2,

• EN 614-1,

• EN 954-1,

• EN 1050,

• EN 61326-1,

• EN 61000-3-3

In order to maintain this condition and ensure safe

operation, the operator must observe all the in-

structions and warnings contained in this instruc-

tion manual.

The protective devices on both instrument and accessories must neither be removed nor

modified. Only qualified service personnel authorized by Leica may repair the instrument

and access its internal components.

3.2 Warnings

The safety devices installed in this instrument by the manufacturer only constitute the basis for

accident prevention. Primarily responsible for accident-free operation is above all the institution

which owns the instrument and, in addition, the designated personnel who operates, services or

repairs the instrument.

To ensure trouble-free operation of the instrument, be sure to comply with the following instruc-

tions and warnings.

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