Motic SILVER 250 Series User manual

Brand: Motic

Model: SILVER 250 Series

Category: Microscopes

Type: User manual

SILVER250 SERIES

INSTRUCTION MANUAL

ENGLISH

SILVERLINE

GENERAL SAFETY INSTRUCTIONS

Please be sure to read these instructions before using the biological microscope. Additional

information is available upon request from our maintenance department or authorized

agency. To ensure safe operation and guarantee good performance of the microscope please

pay attention to the precautions and warnings specified in the Operation Instructions.

INSTRUMENT SAFETY

The SILVER250 Series biological microscope has been designed, manufactured and

inspected according to the EN 61010-1:2001 Safety Requirements for Electrical Equipment

for Measurement, Control and Laboratory Use.

UNPACKING, TRANSPORTATION & STORAGE

The original shipping container, a foam box in a fiberboard carton, should be kept for use in

long term storage or return shipment. When unpacking, please check the components

according to the packing list. Please comply with the temperature requirements for transport

and storage specified in the appendix of this manual. Set up, use and store the unpacked

microscope on a firm and flat workbench. Please do not touch the optical lens surfaces.

WASTE DISPOSAL

Important: Any damaged biological microscope must not be treated as general waste; it

should be disposed of according to relevant regulations.

OPERATION

When using the biological microscope, please pay attention to the following safety instruc-

tions:

• If it is used for any purpose other than the specified ones, including any individual compo-

nent or part, the manufacturer will not take any responsibility.

• After-sales service or repair done by unauthorized personnel will void the warranty.

• Anyone who uses the instrument should receive instruction on the proper handling of the

instrument and safety practices for microscopy. The biological microscope shall be placed

only on a firm, flat workbench for operation.

• Since the biological microscope is a precision instrument, improper operation will impair or

spoil its performance.

• The power unit is integrated in the main unit of the biological microscope: the grid supply

voltage is within 100-240V~50Hz.

The biological microscope must be connected only to the normal power socket with a

grounding terminal. Any extension cord without ground protection is not allowed to avoid

failure of the protection function.

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SAFETY INSTRUCTIONS

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OPERATION

If there is any electrical failure (of the fuse system, ground protection or transformer), turn

off and unplug the unit immediately. Make sure the microscope is set aside so it will not be

used again and contact the Motic service department or a Motic microscope repair agency

to have it repaired.

Please be sure to turn off the power before opening the instrument to replace LED illuminator

or replace the fuse! Only use a fuse for the rated current. Safety instructions for the use of

immersion oil.

Safety instructions for the use of immersion oil:

• Immersion oil is irritating to skin; avoid contact with skin, eyes and clothing.

• Skin contact: wash with soap and plenty of water until the immersion oil is completely

removed.

• Eye contact: flush immediately with plenty of water for at least 5 minutes. If irritation

persists, seek medical advice.

• Dispose of immersion oil properly. Do not discharge into surface water or sewage.

The biological microscope is not equipped with any special device to protect against corro-

sive, latent infective, toxic, radioactive or other hazardous samples. Therefore, when examin-

ing any such sample you must comply with the relevant laws and regulations, in particular

the provisions related to accident prevention.

QUALITY WARRANTY

The SILVER250 Series biological microscope and the attached accessories are only allowed

to be used for microscope examination as described in this manual. The manufacturer takes

no responsibility for any other use.

• The manufacturer guarantees that the product is free from any defect in material or

workmanship on the date of delivery.

• If any defect is found, notify the manufacturer immediately.

• Upon receipt of the Notification of Defect as described above, the manufacture is responsi-

ble to solve the problem either by repairing the defective instrument or replacing it with a new

instrument of the same model.

• The manufacturer provides no warranty for any failure or defect due to normal wear and

tear or improper use of the product.

• The manufacturer takes no responsibility for any damage caused by operation error,

negligence or unauthorized dismantling of the instrument, or the use of spare parts from

other manufacturers.

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SAFETY INSTRUCTIONS

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Eyepieces

Trinocular head

Diopter adjustment

Head locking set screw

Nosepiece

Arm

Objective

Slide holder

Stage

Condenser

Mechanical stage control

Coarse Focus Knob

Fine Focus Knob

Illuminator

Illuminator On/Off Switch

Base

Light intensity control

SILVER253

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SILVER250 SERIES

The SILVER250 Series stands out for its innovation, quality, and student-proof features,

offering a wide range of microscopes for educational purposes in high schools up to advanced

high school biology.

This range of microscopes has cordless LED illumination that provides white light with

virtually no heat and a built-in carrying handle. Choose among our models the one that fits

your application best: monocular or binocular, with stage clips or with built-in mechanical

stage, lead free Achromatic or Semi-Plan objectives.

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APPLICATION

The SILVER250 Series biological microscope is designed for microscopic observation of thin

specimens with transmitted, visible light.

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SILVER250 SERIES

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FIRST TIME USE

Before installing and using the biological microscope, make sure to read carefully the Safety

Instructions.

1. When unpacking and handling, please do not touch the optical surfaces. After unpacking,

place the biological microscope on a flat workbench and remove any foam padding or spacer

used to prevent vibration during transportation.

2. Connect the cable/ charger to the power supply. Before plugging in, keep in mind that the

working voltage of the biological microscope shall be the same as the supply voltage.

3. Turn on the power switch at the back of the arm.

Note: Make sure that the brightness control is in the minimum position before turning on or

off the power switch.

4. Rotate the brightness control to the desired illumination.

5. After use, turn the brightness control to the minimum position, and then turn off the power

and put on the dustproof cover.

6. The coarse focus tension has been set at the factory, but can be readjusted as required.

INSTRUMENT AND ITS MAJOR FEATURES

Major features of the instrument include:

• Built-in LED illumination with brightness adjustment.

• Coaxial coarse and fine focus adjustment with coarse focus tensi on control.

• 75mm x 50mm mechanical stage with slide clips.

• Quadruple revolving nosepiece with ball bearings, thread pitch 0.8”.

• Objectives: 4X, 10X, 40X and 100X (oil immersion), applicable for brightfield, applicable

for darkfield (10X and 40X) and Phase contrast objectives 10X and 40X.

• Field number of 10X eyepiece is 20; high point design for observers with glasses.

•Ergonomically designed binocular tubes with observation angle of 30°; adjustable interpu-

pillary distance

FIRST TIME TO USE & OPERATION

100XRD Objective

Immersion Oil

Slide

OPERATION OF THE BIOLOGICAL MICROSCOPE

Interpupillary distance adjustment

1. While looking through the microscope, grasp the eyetubes and move them on their hinges

until the two circular fields in the observation field coincide with each other.

2. If several people will be using the same microscope, each user can record the correct

interpupillary distance for them from the scale. The microscope can then be quickly reset to

the correct distance.

Setting bright field illumination

The SILVER250 Series biological microscope with adjustable condenser can do as

follow:

• Raise the stage by turning the coarse focus knob.

• Completely lower the condenser carrier by turning the condenser focus knob.

• Turn the condenser focus knob to raise the condenser up to the working position.

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FIRST TIME TO USE & OPERATION

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OIL IMMERSION

When light rays pass through air from the specimen to the objective lens, they are distorted

slightly, a phenomenon known as refraction. This is usually not a problem at a magnification

of 400X or lower. However, at a magnification of 1,000X and above, refraction becomes

problematic. This problem is reduced significantly by placing a thin layer of very clear,

viscous oil between the slide and tip of the objective lens. The result is a much clearer image

at 1,000X because the oil has the same light transmitting properties as glass. Using oil slight-

ly increases the resolution and brightness of the image. Usually a very thin slide (size #1) is

used for oil immersion because at this magnification, the working distance is very small and

is critical to focusing the specimen. Good quality glass (not plastic) cover slips should be

used. If their thickness is over 0.17mm, the objective will not resolve properly, because the

specimen cannot be moved close enough to the objective lens to be in focus. Place a tiny

amount of oil (only 1 drop should be sufficient) onto the slide prior to rotating the 100XRD

objective into position. It is essential to thoroughly clean the objective tip after use to prevent

damage and to ensure that an image can be seen clearly the next time the objective is used.

Please contact Motic Optical or your authorized Motic dealer for the appropriate immersion

oil to use.

IMPORTANT: The working distances of the 40XRD and 100XRD objectives to the slide

surface are very small and although these objectives are sealed to prevent oil contamination,

it is a good practice to avoid dragging these objectives through an oiled slide. The 100XRD

oil immersion lens on Motic microscopes has a spring-loaded end to prevent cracking the

cover slip upon its initial contact. Once this zone of safety is exceeded by moving the slide

further toward the lens, a point can be reached where damage will occur. Always make a

practice of frequently checking the position of the lens on the slide. Note that a 100XRD

objective requires an iris diaphragm for brightfield oil immersion microscopy.

DIGITAL PHOTOGRAPHY

The SILVER252iX model feature a built-in 2592X1944 pixel digital camera to capture still

images or video clips on to a computer. In order to use the camera, the imaging software

must first be installed on a computer. The minimum computer requirements to use the

camera is having an available USB 2.0 port, Windows or Mac OS X operating system installed

on the computer, 1GB of RAM, 1GB free hard drive space and 2Ghz CPU. Complete instruc-

tions on how to use the software is included on the USB flash disk that was packaged with

the SILVER250 Series microscopes.

• Connect the USB cable to the port at the back of the digital microscope head and to an

available USB 2.0 port on a computer.

• Install the Motic Images Plus Imaging software on the computer you will be using with the

microscope. The software should automatically detect and install the correct driver.

IMPORTANT: The next time you connect the SILVER250 Series microscope to a computer,

make sure the USB cable is connected to the same USB port that was used during initial

installation or the software driver will have to be re-installed).

• After the software is installed, start the Motic Images Plus program and click on the MID

icon (6th icon from the top left or click on “File” then “MID”) to view a live image.

• The default set is automatic white balance mode, when need to make up for any uneven

illumination mode, you can set manually by clicking:

Advanced Settings | Advanced Options | Equipment Properties | White Balance to solve the

light problems

• Bring the specimen back into the field of view and use the microscope’s focusing controls

to bring the specimen into focus.

• Click on the camera icon in the upper right corner of the screen to switch to the “Capture”

toolbar. An image can be captured by clicking on the “Capture” box. A video clip can be

recorded by clicking the “Record” box.

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FIRST TIME TO USE & OPERATION

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MAINTENANCE

The biological microscope is limited to the following maintenance only:

• Turn off the power switch after use, and put on the dustproof cover after the microscope

has cooled down.

• Do not operate the microscope in a room with humidity higher than 75%.

• Remove dust or ordinary dirt on optical lens surfaces with a brush, rubber suction bulb and

a moistened lens tissue.

• Use only optical lens tissues and optical lens cleaner (see below). Never clean a lens with

a dry optical lens tissue. Be sure to remove any dust before using lens tissue and cleaner.

• To remove stubborn oily or lipoid dirt (such as immersion oil or fingerprints), dip the lens

tissue into a 3 to 7 ethanol-ether mixture or a commercially available optical lens cleaning

solution and then use it to wipe off the dirt.

• When cleaning an optical lens surface, wipe gently in a circle from the center to the edge.

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MAINTENANCE & TROUBLESHOOTING

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CAUSE

Can not see the whole field

Nosepiece is not locked into the slot

Condenser is not set properly

Low resolution

Poor image contrast

Poor LED illumination

Wrong thickness of cover slip for 0.17 transmitted-light

objective

No immersion oil or non-specified immersion oil for

100X/(oil immersion) objective

Aperture (iris) diaphragm is not set accurately

Incorrect opening of aperture diaphragm

PROBLEM

REMEDY

Bubbles in the immersion oil

Immersion oil or stain left on the front lens of dry objective

Dirt or dust on the optical surface of objective, eyepiece,

condenser or color filter

Poor LED illumination

Rotate the nosepiece to lock into the slot

Set the condenser properly

Set the aperture (iris) diaphragm accurately

Set the opening of aperture diaphragm accurately

Focus the condenser properly

Use the standard 0.17 thick cover slip

Use the immersion oil supplied with the instrument or

go to buy cedar oil for microscope when supplied

immersion oil used up

Add some immersion oil or rotate the nosepiece back and

forth to remove bubbles

Clean the front lens of dry objective (see maintenance

above)

Power plug is not plugged into the socket properly

LED illuminator damaged

Insert the power plug into the socket and turn on the power

Replace LED illuminator

TROUBLESHOOTING

CAUTION

NEVER DISASSEMBLE MECHANICAL OR OPTICAL COMPONENTS. THIS SERVICING SHOULD ONLY BE DONE BY AN

AUTHORIZED MOTIC TECHNICIAN. THE WARRANTY WILL BE NULL AND VOID IF THE MECHANICAL OR OPTICAL COM-

PONENTS ARE DISASSEMBLED BY A NON-MOTIC DEALER.

LED REPLACEMENT

The SILVER250 Series is equipped with a 3 watt LED illumination system. The life of the LED

may vary depending on use and intensity. To prolong the life of the LED, you should always

turn off the unit when not in use. It is important that you only use a Motic replacement LED

because it is integrated on to a circuit board. This LED has been tested and approved for life

span, color temperature and brightness.

Make sure the microscope is unplugged before replacing the LED.

• Remove the eyepiece(s) from the head if they are not secured in place so they do not

accidently fall out of the microscope. Remove the slide that may be on the stage.

• Turn the microscope on its side. Remove the 4 screws on the bottom of the microscope.

Remove the base cover to access the LED.

• The LED is integrated on to a circuit board. This LED circuit board is held into the illumina-

tor housing by 2 screws. Remove the 2 screws from the illuminator housing to remove the

LED circuit board.

• Unplug the LED’s power wire from the circuit board attached to the base .

• Reverse the steps listed above to install the new LED.

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LED REPLACEMENT

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TECHNICAL PARAMETERS

APPENDIX

Biological microscope main unit w/ monocular tube

SILVER250 Series monocular microscopes

Altitude

Transport (within package): Permissible environment temperature

Storage:

Permissible environment temperature

Permissible relative humidity

DIMENSION (W × L × H)

≈ 188 × 395 × 377 mm

≈ 6.6 KG

-40 ~ +70ºC

Below 31ºC , max. humidity is 80%;

at 40ºC , linearly decreases to 50%

Below 2000m

WEIGHT

ENVIRONMENTAL CONDITIONS

Operation:

Permissible environment temperature

Permissible relative humidity

+5 ~ +40ºC

Below 31ºC , max. humidity is 80%;

at 40ºC , linearly decreases to 50%

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TECHNICAL PARAMETERS

APPENDIX

LED illumination:

Color temperature

Even illumination of field

Applicable objective

Coaxial focus adjustment mechanism:

Coarse focus adjustment

Fine focus adjustment

Stroke

LIGHT SOURCES

4000K - 4500K

Diameter 5mm

4X to 100X

OPTO-MECHANICAL PARAMETERS

42mm/rotation

0.2mm/rotation

22mm

Nosepiece Manual quadruple nosepiece

Objective:

Finite

Eyepiece

Field number 20mm

Thread pitch 0.8”

Assembly diameter 30mm

WF 10x/20

Stage:

Dimension (L × W)

Stroke (L × W)

Coaxial focus knob

Position of vernier

Slide clip

1.25 Abbe condenser

140 × 140mm

75 × 50mm

On the right

On the left of movable clip

Used for 4X ~ 100X objectives

Binocular tube 30°/20:

Length of mechanical tube

Maximum field number

Hinge type interpupillary distance adjustment range

Observation angle

Infinite

20mm

48 to 75mm

30°

Triocular tube 30°/20:

Length of mechanical tube

Maximum field number

Hinge type interpupillary distance adjustment range

Observation angle

Splitting ratio

Dimension of the third tube joint

Infinite

20mm

48 to 75mm

30°

50:50

38mm

In the registered product standard, the electrical safety features of thebiological microscope are: over voltage category II; pollution grade

2. The safety requirement test method and inspection rules for this standard product are based on EN 61010-1:2001.

OPERATING PARAMETERS

Protection grade

Ingress protection

Electrical safety

Pollution index

Overvoltage category

Rated supply voltage

Rated supply frequency

Input power

IP20

Conforms to GB 4793.1-2007 / IEC 61010-1:2001

100-240 V

50~60 Hz

36VA

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SILVERLINE WARRANTY

Motic assures that the microscope in respect of mechanical and optical parts is guaranteed against defects in material and workmanship

for ten years. Electrical and electronical components are covered for two years after purchase. Normal wear, routine maintenance, light

bulbs, power supplies, rechargers, batteries, fuses, cords, add-on accessories, damage resulting from repair by unauthorized parties,

accident, alteration, shipping, misuse or abuse is not covered. Warranty service is provided by Motic authorized technicians. Determination

of warranty is at the technician’s discretion.

Other than set forth above, Motic hereby disclaims all warranties, expressed or implied, of fitness for a particular purpose.

Defective products covered by the warranty will be repaired free of charge when they are returned to:

Motic Europe, S.L.U.

Pol. Ind. Les Corts · C. Les Corts, 12 · 08349 Cabrera de Mar · Barcelona · Spain

T. +34 93 756 62 86 | F. +34 93 756 62 87 | info@moticeurope.com

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Motic Instruments Inc. (Canada)

130 - 4611 Viking Way. Richmond, BC V6V 2K9 Canada

Tel: 1-877-977 4717 | Fax: 1-604-303 9043

Motic Deutschland GmbH (Germany)

Christian-Kremp-Strasse 11, D-35578 Wetzlar, Germany

Tel: 49-6441-210 010 Fax: 49-6441-210 0122

Motic Hong Kong Limited (Hong Kong)

Rm 2907-8, Windsor House, 311 Gloucester Road, Causeway Bay, Hong Kong

Tel: 852-2837 0888 | Fax: 852-2882 2792

Motic Europe, S.L.U. (Spain)

Polígon Industrial Les Corts, C. Les Corts, 12 08349 Cabrera de Mar, Barcelona, Spain

Tel: 34-93-756 6286 | Fax: 34-93-756 6287

*CCIS

is a trademark of Motic Incorporation Ltd.

Design Change: The manufacturer reserves the right to make changes in instrument design in

accordance with scientific and mechanical progress, without notice and without obligation.

Designed in Barcelona (Spain)

February 2021

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