Contents
CONTENTS............................................................................................................................................................................III
LIST OF FIGURES...................................................................................................................................................................V
LIST OF TABLES.....................................................................................................................................................................V
1. BACKGROUND...................................................................................................................................................................1
1.1 WHAT IS FLUORESCENCE?....................................................................................................................................................2
1.2 MEASURING ORGANIC MATTER USING FLUORESCENCE..............................................................................................3
2. INSTRUMENT SPECIFICATIONS......................................................................................................................................4
2.1 INSTRUMENT......................................................................................................................................................................4
2.2 APPLICATIONS AND USER MODES........................................................................................................................................6
2.2.1 Real-time manual data collection.......................................................................................................................6
2.2.2 Unattended Logging.........................................................................................................................................6
2.2.3 Telemetry................................................................................................................................................................6
2.2.4 Online, Real-time Monitoring..............................................................................................................................6
2.3 STATUS INDICATOR LIGHTS..............................................................................................................................................7
2.4 ACCESSORIES.......................................................................................................................................................................8
2.4.1 Proteus High Range Dilution Sampling System.......................................................................................8
2.4.2 Remote Telemetry Systems .........................................................................................................................8
3. COMMUNICATION SOFTWARE........................................................................................................................................9
3.1 SYSTEM REQUIREMENTS......................................................................................................................................................9
3.1.1 Control Software and Serial Adaptors.............................................................................................................9
3.1.2 Trouble Shooting...............................................................................................................................................9
3.2 INTERFACE SCREEN...........................................................................................................................................................10
3.3 HOT BUTTONS...................................................................................................................................................................10
3.4 DROP DOWN MENUS.......................................................................................................................................................11
3.4.1 PC Menu..............................................................................................................................................................11
3.4.2 Proteus Menu.....................................................................................................................................................11
3.4.3 Custom Parameters.......................................................................................................................................12
4. INSTALLATION.................................................................................................................................................................14
4.1 OPEN WATER INSTALLATION.............................................................................................................................................14
4.2 FLOW CELL INSTALLATION...........................................................................................................................................14
5. MAINTENANCE...............................................................................................................................................................15
5.1 ROUTINE MAINTENANCE AND CLEANING................................................................................................................15
5.2 TURBIDITY SENSOR AND WIPER ARM.....................................................................................................................15
5.3 FLUOROMETER MAINTENANCE.................................................................................................................................16
5.4 MAINTENANCE OF OTHER SENSORS........................................................................................................................17
5.4.1 Reference Electrode......................................................................................................................................17
6. CALIBRATION.................................................................................................................................................................18
6.1 BASICS OF PARAMETER CALIBRATION.............................................................................................................................18
6.1.1 Calibration step-by step guide..................................................................................................................19
6.1.2 Calibration Troubleshooting........................................................................................................................20
6.2 CHOOSING CALIBRATION STANDARDS...........................................................................................................................20
6.2.1 Tryptophan Stock Solution..............................................................................................................................22
6.3 THE CALIBRATION LOG..................................................................................................................................................22
6.4 SENSOR RESPONSE FACTOR (SRF).............................................................................................................................23
6.5 UNITS/CONVERSION FACTORS....................................................................................................................................23
6.6 FACTORY CALIBRATION FOR DERIVED PARAMETERS..................................................................................................24
6.7 TURBIDITY CALIBRATION.................................................................................................................................................24
6.8 FLUOROMETER CALIBRATION....................................................................................................................................25
6.8.1 Typical Tryptophan Ranges and respective BOD...............................................................................26
6.8.2 Temperature Compensation.....................................................................................................................26
6.8.3 Tryptophan Turbidity Compensation........................................................................................................27
6.8.4 Verication and Calibration..........................................................................................................................27
III