LI-COR Pearl Trilogy Owner's manual

Category
Supplementary music equipment
Type
Owner's manual
Pearl® Trilogy
Operator’s Manual
CE Marking:
This product (model number 9430) is a CE-marked product. For conformity information, contact LI-COR
Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or
distributor.
珍珠三部曲(型号9430/8500)小动物成像系统
Pearl Trilogy (Model 9430) and Pearl MSI (8500) Small Animal Imaging System
部件名称
Part Name
有毒有害物质或元素
Toxic and Hazardous Substances or Elements
(PB)
(Hg)
(Cd)
六价铬
(Cr(VI))
多溴联苯
(PBB)
多溴二苯醚
(PBDE)
印刷电路板
(PCBs) X O O O O O
机电配件
(Electromechanical Parts) X O O O O O
光学
(Optics) OOOOOO
电缆和电线
(Cables and Wires) X O O O O O
金属部件
(Metal Parts) X O X O O O
塑料零件
(Plastic Parts) OOOOO X
电池
(Batteries) OOOOOO
本表格依据 SJ/T 11364 的规定编制
O = 表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572 规定的限量要求以下
indicates that the content of the toxic and hazardous substance in all the Homogeneous Materials of the part is below the concentration limit
requirement as described in GB/T 26572
X = 表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572 规定的限量要求
indicates that the content of the toxic and hazardous substance in at least one Homogeneous Material of the part exceeds the concentration limit
requirement as described in GB/T 26572
Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual. The
safety of this product cannot be guaranteed if the product is used in any other way than is specified in this
manual. The Pearl® Imager is intended to be used by qualified personnel. Read this entire manual before using
the Pearl Imager.
Equipment Markings:
The product is marked with this symbol when it is necessary for you to refer to the
manual or accompanying documents in order to protect against damage to the product.
The product is marked with this symbol when a hazardous voltage may be present.
Manual Markings:
WARNING Warnings must be followed carefully to avoid bodily injury.
CAUTION Cautions must be observed to avoid damaging your equipment.
NOTE Notes contain additional information and useful tips.
IMPORTANT Information of importance to prevent procedural mistakes in the operation of the
equipment or related software. Failure to comply may result in a poor experimental
outcome but will not cause bodily injury or equipment damage.
Federal Communications Commission Radio Frequency Interference Statement
WARNING: This equipment generates, uses, and can radiate radio frequency energy and if not installed in
accordance with the instruction manual, may cause interference to radio communications. It has been tested
and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC
rules, which are designed to provide a reasonable protection against such interference when operated in a
commercial environment. Operation of this equipment in a residential area is likely to cause interference in
which case the user, at his own expense, will be required to take whatever measures may be required to
correct the interference.
Page 4
Notice
The information contained in this document is subject to change without notice. LI-COR MAKES NO
WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO
THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.
LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection
with the furnishing, performance, or use of this material.
This document contains proprietary information that is protected by copyright. All rights are reserved. No part
of this document may be photocopied, reproduced, or translated to another language without prior written
consent of LI-COR, Inc.
Printing History
Publication Number 977-19834
Printed: June 2016
Revised: 2022-03-22
LI-COR Biosciences is an ISO 9001:2015 certified company. © 2022 LI-COR, Inc. Specifications subject to
change. LI-COR, Pearl, In-Cell Western, IRDye, Odyssey, and Image Studio are registered trademarks of LI-COR,
Inc. in the United States and other countries. All other trademarks belong to their respective owners. The
Odyssey Infrared Imaging Systems and IRDye reagents are covered by U.S. patents, foreign equivalents, and
patents pending.
Global Headquarters
4647 Superior Street • Lincoln, NE 68504
Toll free: 800-645-4267 • Fax: +1-402-467-0819 • [email protected] licor.com/bio
Regional Offices
LI-COR Biosciences GmbH
Siemensstraße 25A • 61352 Bad Homburg • Germany
Phone: +49 (0) 6172 17 17 771 • Fax: +49 (0) 6172 17 17 799 • [email protected]
LI-COR Biosciences UK Ltd.
St. John’s Innovation Centre • Cowley Road
Cambridge • CB4 0WS
United Kingdom • Phone: +44 (0) 1223 422104 • Fax: +44 (0) 1223 422105 • [email protected]
LI-COR Biotechnology Distributor Network: www.licor.com/bio/distributors
Page 5
Chapter 1: Installation and Safety Information
Safety Considerations 9
Laser Safety 9
Safety Interlocks 10
Placement in the Laboratory 11
Ambient Laboratory Conditions 11
Instrument Ventilation 12
Room Ventilation 12
Space Requirements 12
Level the Imager 12
Moving the Imager 12
Anesthesia Safety Considerations 13
Oxygen Safety Considerations 13
Chemical Safety 13
Use of DMSO and Other Strong Solvents 13
Electrical Considerations 15
Power Cords and Disconnecting Power 15
Fuse Replacement 15
Chapter 2: System Overview
External Panels and Controls 17
Front Panel 17
Right-side Panel 18
Rear Panel 19
Imaging Drawer 19
Imaging Drawer Safety 19
Installing the Standard Imaging Bed 20
Computer Connections and Networking 24
Image Studio™ Software Features 25
Optical System Description 25
Near-Infrared Detection 27
Bioluminescent Detection 27
Overview of Infrared In Vivo Imaging 27
References 29
Pearl® Trilogy
Imaging
System
Table of Contents
Page 6
Chapter 3: Connecting an Anesthesia System
Installing the Rotameter and Charcoal Filter 31
Connecting an Alternate Scavenging System 33
Connecting an Anesthesia System 33
Checking the Imaging Bed Connections 33
Starting the Anesthesia Gas Flow 35
Stopping the Anesthesia Gas Flow 35
Flow Path for Anesthesia Gas 35
Chapter 4: Operation
Overview 37
Cleaning the Imaging Bed 37
Setting the Heater Plate Temperature 38
Starting the Anesthesia Gas 38
Placing Mice on the Imaging Bed 40
Using Restraints 40
Using a Mouse Blanket 41
Imaging Tips 41
Hair vs. Shaved Animal 41
Diet Considerations 41
Mouse Recovery Procedure 42
Chapter 5: Routine Maintenance
Routine Maintenance 45
Cleaning the Fan Filter 45
Nose Cone Replacement 46
Replacing Heater Plate Contact Pins 46
Desiccant Replacement 47
Pearl® Imager Installation Instructions 47
Front Panel Warning Light Indicates an Error 49
Resetting the Instrument 49
Obtaining Technical Support 49
Exporting ImageStudio Software Log Files 49
Chapter 6: Pearl Docking Station
Description 51
Assembling the Pearl Docking Station 52
Docking Station Operation 56
Routine Maintenance 58
Docking Station Specifications 58
Chapter 7: Pearl Clean Box
Page 7
Description 59
Installation 59
Connecting a HEPA Filter to the Docking Station 59
Connecting a HEPA Filter to the Pearl® Imager 60
Nose Cone Installation and Replacement 60
Sanitizing the Pearl Clean Box 61
Clean Box Operation 61
Maintenance of the Clean Box Window 62
Scratches 62
Smudging 62
Chemicals 63
Cleaning Procedure 63
Replacing Heater Plate Contact Pins 63
Chapter 8: Appendices
Specifications 65
Embedded Firmware Source Code 66
Page 8
Chapter 1: Installation and Safety Information
Safety Considerations
Laser Safety
The Center for Devices and Radiological Health (CDRH) was established in October, 1982, by the
U.S. Food and Drug Administration (FDA) to protect the public health in the fields of medical
devices and radiological health.
Manufacturers of products subject to performance standards under the Radiation Control for Health
and Safety Act of 1968 are required to furnish various reports to the CDRH.
The Pearl Imager is certified as a Class I laser product. This means that hazardous laser radiation is
not emitted outside the instrument. Radiation emitted inside the Pearl Imager is confined within
protective housings and external covers. A series of safety interlocks ensures that the laser beam
cannot escape during any phase of user operation.
Two lasers emit at 785 nm and the other two at 685 nm. The 785nm lasers have a peak power rating
of 1 watt. The 685nm lasers have a peak power rating of 0.5 watt. The 685 nm laser emits visible laser
radiation - direct exposure to either beam may cause eye damage. Laser radiation from each laser
uniformly illuminates the target area, which is the imaging bed (Figure 2-5) in the imaging drawer.
The CDRH implemented regulations for laser products on August 1, 1976 (CDRH radiation
performance standard 21, Code of Federal Regulations Chapter 1, Sub-chapter J). Compliance for
products marketed in the United States is mandatory. This product complies with CDRH radiation
performance standard 21 CFR Chapter 1, Sub-chapter J. Ce produit est conforme à la performance de
rayonnement CDRH chapitre CFR norme 1 Sous-chapitre J. The label that must be attached to laser
products marketed in the United States is Figure 1-1 and is located on the rear panel of the Pearl
(Figure 1-2), indicating compliance with CDRH regulations.
WARNING: Use of controls or adjustments or performance of procedures other than those specified
herein may result in hazardous radiation exposure.
AVERTISSEMENT: L'utilisation des commandes ou le réglage ou la performance des procédures
autrement qu'il a été indiqué dams le présent texte peut résulter en de dangereuses expositions aux
radiations.
Pearl® Trilogy
Imaging
System
Page 9
Chapter 1: Installation and Safety Information
Figure 1-1. CDRH and IEC 60825-1:2007 regulation
compliance label. See Power Cords and Disconnecting
Power on page15 for more information about
disconnecting power before maintenance or servicing.
Figure 1-2. Location of CDRH compliance label on the
Pearl®ImagingSystem. Note: The appearance of your
instrument's back panel may vary slightly from the one
shown.
DANGER - CLASS 4 VISIBLE AND INVISIBLE LASER
RADIATION WHEN OPEN AVOID EYE OR SKIN
EXPOSURE TO DIRECT OR SCATTERED RADIATION
DANGER VARNING
DANGER - APPAREIL LASER
DE CLASSE 4. RAYONNEMENT
LASER VISIBLE ET INVISIBLE
EN CAS D’OVERTURE. EVITER
L’EXPOSITION DE L’OEIL OU DE
LA PEAU AU RAYONNEMENT
DIRECT OU DIFFUS.
VARNING – KLASS 4 SYNLIG
OCH OSYNLIG LASERSTRÅLNING
NÄR ENHETEN ÄR ÖPPEN.
UNDVIK EXPONERING AV ÖGON
OCH HUD I DIREKT ELLER
REFLEKTERAD STRÅLNING.
Figure 1-3. Class 4 laser warning label.
Safety Interlocks
Where there is a potential for class 4 laser radiation behind a given panel, the label in Figure 1-3 is
affixed to the panel. The label shown in Figure 1-3 is affixed to the Pearl Imager in two locations; one
on the rear lower access panel and one on top of the camera hood (Figure 1-4).
Figure 1-4. Location of class 4 laser warning labels located (a) on the camera hood under the top panel of the Pearl
Imager instrument enclosure, and (b) on the exterior of the rear lower access panel.
Page 10
Pearl® Trilogy Imaging System
The Pearl Imager has two safety interlocks that prevent exposure to laser radiation when the Pearl
imaging drawer is opened during operation. Do not attempt to defeat these interlocks. The drawer
interlocks are located in the back of the instrument behind the rear lower access panel, which is also
protected by interlocks. This second set of interlocks prevents exposure to laser radiation if the rear
lower access panel is removed during operation. These interlocks are also located behind this back
access panel (Figure 1-5). Do not attempt to defeat these interlocks (Figure 1-6).
Figure 1-5. Location of laser interlock warning label behind the rear lower access panel.
DANGER - CLASS 4 VISIBLE
AND INVISIBLE LASER
RADIATION WHEN OPEN
AND INTERLOCKS
DEFEATED AVOID EYE
OR SKIN EXPOSURE TO
DIRECT OR
SCATTERED
RADIATION
VARNING – KLASS 4 SYNLIG
OCH OSYNLIG LASERSTRÅLNING
NÄR ENHETEN ÄR ÖPPEN OCH
FÖRREGLING ÅSIDOSATTS.
UNDVIK EXPONERING AV
ÖGON OCH HUD I
DIREKT ELLER
REFLEKTERAD
STRÅLNING.
DANGER - APPAREIL LASER DE
CLASSE 4. RAYONNEMENT LASER
VISIBLE ET INVISIBLE EN CAS
D’OVERTURE ET LORSQUE LE
DISPOSITIF DE SECURITE EST
NEUTRALISE. EVITER L’EXPOSITION E L’OEIL
OU DE LA PEAU AU RAYONNEMENT
DIRECT OU DIFFUS.
Figure 1-6. Laser interlock warning label.
Placement in the Laboratory
The Pearl Imaging System weighs approximately 25 kg (55 lb). The instrument should be placed on a
laboratory bench that is sufficiently sturdy to bear its weight. The bench should also be able to bear
the weight of the computer if the computer is to be installed beside the Pearl Imaging System
instrument.
Ambient Laboratory Conditions
Place the Pearl Imaging System away from external heat sources (furnaces, windows, etc.). Additional
heating can cause high temperatures within the enclosure. The heater plate in the Pearl Imaging
System imaging bed cannot cool the imaging bed if ambient temperature is above the set point for
the imaging bed heater plate.
Page 11
Chapter 1: Installation and Safety Information
Place the instrument away from sinks or other sources of water that pose a shock hazard.
Recommended operating conditions are 15-35 °C and a dew point not greater than 22 °C to prevent
condensation on the laser assemblies, which are cooled during operation.
Instrument Ventilation
The air intake fan on the back of the Pearl®ImagingSystem (Figure 2-4) is used to cool the internal
electronics and maintain fresh air circulation throughout the internal volume of the instrument.
Fresh air circulation prevents an internal buildup of anesthesia gas or oxygen. The air intake is
filtered and the filter should be periodically cleaned (Routine Maintenance on page45). Make sure
there is plenty of room behind the instrument (Figure 2-5) so that the air intake is not blocked and
the filter is easily accessible for cleaning.
Room Ventilation
A room with a minimum of 15 air changes per hour (non-recirculating) is required when using
isoflurane anesthesia gas.
Space Requirements
The Pearl Imaging System instrument requires an area approximately 41 cm W (16") x 41 cm D (16")
x 66 cm H (26"). Depth with the imaging drawer open is 63.5 cm (25”).
Level the Imager
Level the instrument to prevent the imaging drawer from scraping the lab bench or table in front of
the instrument and to keep the instrument from tilting.
The instrument can be leveled by turning the threaded metal inserts located in each of the 4 plastic
feet (Figure 1-7).
Figure 1-7. Turn feet to adjust height.
Moving the Imager
LI-COR recommends that you do not move your imager. Instead, please contact LI-COR Technical
Support for options.
LI-COR cannot be held responsible for any damage incurred while moving, transporting, or
shipping the Pearl Imager, even if an existing warranty and/or service contract applies. LI-COR
cannot be held responsible for any injury incurred while moving, transporting, or shipping
the Pearl Imager.
Page 12
Pearl® Trilogy Imaging System
Anesthesia Safety Considerations
WARNING: Isoflurane gas is a human inhalation hazard that needs to be ventilated away
from workers. It can cause coughing, sore throat, dizziness, drowsiness, headache, and
unconsciousness. Pregnant women should not work in an environment where there is any
potential isoflurane risk.
AVERTISSEMENT:
Le gaz isoflurane est un danger pour le système respiratoire humain et il doit être dispersé par
ventilation loin des ouvriers. Il peut provoquer la toux, la gorge endolorie, le vertige, la
somnolence, le mal de tête, et l'inconscience. Les femmes enceintes ne devraient pas travailler
dans un environnement où il existe un danger d'inhalation d'isoflurane.
Oxygen Safety Considerations
Oxygen gas is a colorless, odorless, oxidizing gas. If O2is used as a carrying gas for the anesthesia gas,
the chief physical hazard associated with the release of oxygen is its oxidizing power, which can
greatly accelerate the burning rate for combustible materials. Keep the room well ventilated with
fresh air by observing the room ventilation recommendations above.
Chemical Safety
LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory
procedures be followed at all times. Be aware of the hazards associated with any chemical before you
begin work.
The LI-COR®Pearl Imaging System should not be used with any radioactive materials.
Use of DMSO and Other Strong Solvents
CAUTION: Strong solvents, including DMSO, will permanently discolor the imaging bed
surface. Avoid all contact. In addition, any IRDye®products in DMSO will incorporate into
the surface of the imaging bed and will be visible in images.
ATTENTION:
Les dissolvants forts, y compris DMSO, décoloreront de manière permanente la surface du lit
d'image. Évitez tout contact. En outre, tous les produits d'IRDye dans DMSO incorporeront à
la surface du lit et seront évidents dans les images.
Page 13
Chapter 1: Installation and Safety Information
Figure 1-8. Caution labels for DMSO and other strong solvents.
Whenever possible, inject the animal on a separate surgical bed and clean off any exterior dye
product on the animal before transferring the animal onto the Pearl Imaging System imaging bed. In
the event that the imaging bed becomes contaminated with dye, wipe with Nair®hair remover and
thoroughly clean and dry before use.
Page 14
Pearl® Trilogy Imaging System
Electrical Considerations
Power Cords and Disconnecting Power
The Pearl®ImagingSystem is equipped with a 3-wire grounding-type plug. This plug will only fit
into a grounding-type outlet. This is a necessary safety feature. If you are unable to insert the plug
into the outlet, you will need to replace the outlet. Do not defeat the purpose of the grounding-type plug.
Do not locate the Pearl Imaging System where the power cord will be walked on or exposed to water
or chemical spills.
The Pearl Imaging System draws a maximum of 4 amperes at 120V (see Specifications on page65). If
an extension cord is used, make sure the total of the ampere ratings on the instruments plugged into
the extension cord does not exceed the extension cord ampere rating. Also make sure the total
amperage of instruments plugged into the wall outlet does not exceed the amperage capacity for the
outlet (usually 15 or 20 amperes in the United States).
Disconnect power before maintenance or servicing.
Débrancher le pouvoir avant d'entretenir.
See Figure 1-1 on page10 for the location of these statements on the product's regulation compliance
label.
Fuse Replacement
There are no user replaceable fuses in this instrument.
Page 15
Page 16
Chapter 2: System Overview
External Panels and Controls
Front Panel
Power on/off button
Image acquisition 'in
progress' indicator (shown
unlit)
Error condition indicator
(unlit)
Scavenging charcoal filter
Rotameter indicating flow rate
of anesthesia gas
Rotameter flow control valve
Imaging drawer open/close
button
Imaging drawer (closed)
Figure 2-1. Front panel features and controls of the Pearl Imager.
Power On/Off Button: Pressing this button momentarily turns the instrument on and off. Pressing
the button for 5 seconds or more cuts power to the instrument (Routine Maintenance on page45).
While the instrument is starting up or shutting down, the blue indicator light in the power button
blinks. When the blue indicator light is continuously on, the instrument is ready for operation.
Pearl® Trilogy
Imaging
System
Page 17
Chapter 2: System Overview
Note: Instruments with firmware version 1.3.1 and earlier will shut down if you press the power
button, even if the image acquisition light is on. On instruments with newer firmware, the red
error indicator will illuminate if you press the power button while the image acquisition indicator
is on. Hold down the power button to force a shut down.
Green Image Acquisition Light: This light blinks during image acquisition and is continuously on
at other times, as long as communication with Image StudioSoftware is maintained. The light is
off only if communication is lost or Image Studio Software is closed.
Red Error Light: The error light illuminates to indicate that a particular process could not be
completed. See Routine Maintenance on page45.
Rotameter: The rotameter provides flow control for anesthesia gas, as well as inlet connectors to
receive anesthesia gas from the SmartFlow Anesthesia System or a user-supplied anesthesia system.
Scavenging Charcoal Filter: The charcoal filter removes isoflurane gas from the exhaust air stream.
Imaging Drawer Button: This button opens and closes the imaging drawer in normal operation.
The drawer handle on the outside of the drawer can be used to open or close the drawer in cases of
power loss or emergency.
Imaging Drawer: The imaging drawer moves the mouse into position under the camera for
imaging. The imaging drawer contains the imaging bed, which provides heat and anesthesia gas for
the mouse.
Right-side Panel
Figure 2-2. Right-side panel features and controls of the
Pearl Imager.
Figure 2-3. Close up of the Pearl control module showing
the power receptacle and Ethernet connectors (use only
the supplied Ethernet cable). Do not use any other
connector on this panel. Note: The appearance of your
instrument's back panel may vary slightly from the one
shown.
Page 18
Pearl® Trilogy Imaging System
Rear Panel
Rotameter in
storage position
Air fan inlet
Lower rear access
panel
Figure 2-4. Rear panel showing air inlet fan. Keep the inlet unblocked during operation and accessible for routine filter
cleaning (Routine Maintenance on page45).
Imaging Drawer
Figure 2-5. The drawer front tilts forward and shifts
downward automatically when opened, making the
removable imaging bed easier to access.
Imaging Drawer Safety
Be careful not to get your fingers caught between the Pearl®ImagingSystem and the imaging drawer
when the drawer closes. The drawer is specially designed to stop if a certain level of resistance is
detected, so no harm should result, but the experience may be startling.
Page 19
Chapter 2: System Overview
During an unexpected power loss, the drawer button will not function. This may leave a mouse
stranded inside the Pearl Imaging System, or it may result in the imaging drawer being left partially
open or closed. In any case, the drawer can be opened or closed by pulling or pushing on the drawer
handle on the drawer front.
The imaging drawer should typically be opened and closed using the drawer button, but the
drawer handle can be used to manually open or close the drawer. The drawer handle is
especially useful in case of power loss or emergency.
Installing the Standard Imaging Bed
1) Make sure the imaging bed and imaging drawer are clean and dry (Routine Maintenance on
page45) before starting.
2) Make sure a nose cone plug is in place in the nose cone on the imaging bed.
Figure 2-6. Anesthesia gas connectors on the imaging
drawer are closed when the imaging bed is removed but
open immediately when the imaging bed is locked in
place.
WARNING: If anesthesia gas is flowing
to the Pearl Imager, gas will start flowing
to the nose cone immediately after the
imaging bed is locked in place. Make sure
the nose cone plug is in place before
installing the imaging bed.
AVERTISSEMENT:
Si le gaz d'anesthésie coule dans Pearl, le
gaz commencera à couler dans le cône de
nez dès que le lit d'image est installé en
place fermement. Assurez-vous que la
prise de cône de nez est en place avant
d'installer le lit d'image.
Figure 2-7. If the system is pressurized with anesthesia gas, the nose cone plug should be in place before the
imaging bed is locked into the imaging drawer to minimize operator exposure to anesthesia gas.
Page 20
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LI-COR Pearl Trilogy Owner's manual

Category
Supplementary music equipment
Type
Owner's manual

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