Thermo Fisher Scientific Polysine™ Operating instructions

  • I have reviewed the provided document, which is an Instructions For Use for Polysine Slides from Thermo Scientific. This guide details the process of mounting and adhering tissue sections to these slides, focusing on their rapid bonding capabilities and the elimination of need for glue. I'm ready to answer your questions about its contents.
  • What kind of water should I use when floating tissue sections?
    How quickly does tissue bonding begin?
    How should I dry the slides?
• Float 2 to 5 micron thick tissue sections on a pre-heated distilled water bath.
Do not add glue or subbing solution to the bath. Polysine™ slides eliminate
the need for these compounds. (Note: You may try substituting tap water for
distilled water. However, if you begin losing tissue sections, use only distilled
water.)
• Mount your tissue sections carefully. Tissue bonding begins quickly on Polysi-
ne™ slides. Tissue can be repositioned for approximately the first minute, but
thereafter the bonding occurs rapidly and thoroughly.
• Dry slides completely at room temperature by vertically draining them before
heating them in an oven. A microwave oven can also be used for quick adhesi-
on, once the tissue sections are thoroughly dried.
thermoscientifi c.com
Slides & Specialty Glass
Tel. +49 (0) 531 59 00 80
Fax +49 (0) 531 50 97 99
menzel.marketing@thermofi sher.com
© 2015 Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of
Thermo Fisher Scientifi c Inc. and its subsidiaries.
Thermo Fisher Scientifi c
Gerhard Menzel B.V. & Co. KG
Saarbrückener Straße 248
38116 Braunschweig, Germany
Polysine
Instructions For Use
thermoscientifi c.com
Slides & Specialty Glass
Tel. +49 (0) 531 59 00 80
Fax +49 (0) 531 50 97 99
menzel.marketing@thermofi sher.com
© 2015 Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of
Thermo Fisher Scientifi c Inc. and its subsidiaries.
Thermo Fisher Scientifi c
Gerhard Menzel B.V. & Co. KG
Saarbrückener Straße 248
38116 Braunschweig, Germany
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