COMBINATION BIGUANIDE/HYDROGEN
PEROXIDE TEST
COMPONENTS:
Biguanide
1 x 4030 Pipet, Calibrated 0.5 & 1.0 mL, plastic w/cap
1 x R-0976 Biguanide Complexing Reagent
1 x R-0977 Biguanide Indicator Reagent, DB
1 x R-0978 Biguanide Titrating Reagent, DB
Hydrogen Peroxide
1 x R-0601 Molybdate Reagent, DB
1 x R-0664 Bleach Reagent #1, DB
1 x R-0774 Thiosulfate Reagent, DB
Misc.
1 x 5313 Instruction
1 x 9198 Sample Tube, Graduated, 25 mL, plastic w/cap
TO ORDER REPLACEMENT PARTS AND REAGENTS CALL TOLL-FREE 800-TEST KIT
(800-837-8548).
PROCEDURE:
CAREFULLY READ AND FOLLOW PRECAUTIONS ON REAGENT LABELS.
KEEP REAGENTS AWAY FROM CHILDREN.
Biguanide (Drop Test)
1.Rinseandll25mLsampletube(#9198)to10mLmarkwithwatertobetested.
2.Usinga1.0mLpipet(#4030),add0.5mLofR-0976BiguanideComplexingReagent.Swirl
tomix.
3.Add5dropsofR-0977BiguanideIndicatorReagent.Swirltomix.Sampleshouldturnblue. 31LovetonCircle,Sparks,MD21152U.S.A.
800-TESTKIT(837-8548) • 410-472-4340 3/08
4.AddR-0978BiguanideTitratingReagentdropwise,swirlingandcountingaftereachdrop,
untilcolorchangesfrombluetopink-purple.Alwaysholdbottleinverticalposition.
5.MultiplydropsofR-0978BiguanideTitratingReagentby5.Recordaspartspermillion(ppm)
PHMB(polyhexamethylenebiguanide)asproduct.
Hydrogen Peroxide (Drop Test)
1.Rinseandll25mLsampletube(#9198)to25mLmarkwithwatertobetested.
2.Add10dropsR-0664BleachReagent#1.Swirltomix.
3.Add10dropsR-0601MolybdateReagent.Swirltomix.Samplewillturnyellow(yellow-
orange)ifhydrogenperoxideispresent.
4.AddR-0774ThiosulfateReagentdropwise,swirlingandcountingaftereachdrop,untilcolor
changesfromyellow(yellow-orange)tocolorless.Alwaysholdbottleinverticalposition.
5.Multiply dropsof R-0774 Thiosulfate Reagent by 5. Recordas parts per million(ppm)
hydrogenperoxide.
Instr. #5313