Protocol

Avoiding RNase Contamination

Reagents have been tested to ensure they are endo-,

exodeoxyribonuclease, ribonuclease, and phosphatase

free. However, an RNase-free working environment

and RNase-free solutions are also critical factors for

performing successful in vitro transcription.

General recommendations to avoid RNase contamination:

• Maintain a separate area, dedicated pipettors and

and handling with DEPC. Add DEPC to 0.1% (v/v)

nal concentration; incubate overnight at room

temperature and autoclave.

• High quality reagents must be used for buffer solutions.

Buffers containing Tris should be prepared by dissolving

Tris base in DEPC-treated water. Solutions containing

DTT or nucleotides should be prepared by dissolving in

DEPC-treated water and passing the solution through a

0.2 µm lter to sterilize.

• Keep all kit components sealed when not in use and all

thermofisher.com

customerservice.emea.genomics@

thermofisher.com

© 2012 Thermo Fisher Scientic Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientic Inc. and its subsidiaries. Specications,

terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.