IRIS Cytofuge 12 User manual

Brand: IRIS

Model: Cytofuge 12

Type: User manual

IRIS Cytofuge 12 is a versatile cytocentrifuge system that simplifies cell preparation for microscopic examination. It features a removable rotor for easy loading and unloading of samples, programmable operation for precise control of centrifugation parameters, and a variety of accessories for different sample types. With its compact design and user-friendly interface, the IRIS Cytofuge 12 is an ideal choice for laboratories of all sizes.

Cytofuge

12 Cytocentrifuge System

Operator’s Manual

A Division of IRIS International, Inc.

Operator’s Manual

Cytofuge

12 Cytocentrifuge System

Model Number M802

For in-vitro diagnostic use

Product Number

CF12 – Cytofuge 12 for 100-240 VAC, 50/60 Hz

StatSpin and Iris are registered trademarks of IRIS International, Inc.

Printed in U.S.A.

Table of Contents

How to use this manual ...................................................................................... ii

Section 1 ............................................................................................................. 1

Inspect Packaging .......................................................................................... 1

Verify Contents ............................................................................................... 1

Install .............................................................................................................. 1

Connect Power ............................................................................................... 2

Section 2 ............................................................................................................. 3

Principle and Intended Use ............................................................................ 3

Symbols and Definitions ................................................................................. 3

Operator Controls ........................................................................................... 4

Error Indicators ............................................................................................... 5

Accessories .................................................................................................... 5

Section 3 ............................................................................................................. 6

Overview ........................................................................................................ 6

Sample Volumes ............................................................................................ 6

Pre-concentration ........................................................................................... 6

Dilution ........................................................................................................... 6

Section 4 ............................................................................................................. 7

Opening and Closing the Cover ..................................................................... 7

Using the Removable Rotor ........................................................................... 7

Spinning Samples........................................................................................... 8

Programming .................................................................................................. 9

Section 5 ........................................................................................................... 10

Overview ...................................................................................................... 10

Cleaning ....................................................................................................... 10

Checking/Replacing Rotor Seal .................................................................... 11

Checking/Replacing Drive Cone O-rings ...................................................... 11

Checking the Rotor Speed ........................................................................... 12

Troubleshooting ............................................................................................ 12

Service ......................................................................................................... 13

Section 6 ........................................................................................................... 14

Specifications ............................................................................................... 14

References ................................................................................................... 15

Iris Sample Processing Warranty ................................................................. 16

Limitation of Liability ..................................................................................... 16

How to use this manual

This manual along with information contained on product labels and in package inserts should provide

you with all the information you need to operate and maintain the Cytofuge 12 Cytocentrifuge system.

When the information requires special attention, a caution symbol appears next to the italicized

text.

Please pay close attention to the instructions that accompany the notes and symbols as well as the

standard laboratory practices outlined by your facility and local regulatory agencies. The table below lists

all the CAUTIONS and WARNINGS for the Cytofuge

12 Cytocentrifuge.

WARNING - Do not expose the cytocentrifuge or its rotor to strong or concentrated acids, bases or

oxidizing agents, aromatic or halogenated hydrocarbons, esters or ketones.

WARNING - Do not use the supplied line cord outside of North America. Use a power cord with a

grounded male connector suitable for the power outlet being used, which is rated at 3.0 Amps or more

and has an IEC320/CEE22 female connector to attach to the cytocentrifuge.

WARNING - Maintain a 300 mm (11.8 inches) clearance envelope around cytocentrifuge during

operation. Do not deposit any potentially hazardous materials within the CLEARANCE ENVELOPE.

WARNING - Inspect instrument for cracks or any physical damage to housing, cover, lid and rotor.

Damage may result in unsafe operation; discontinue use until repairs have been performed.

CAUTION - Do not overfill the concentrators. Addition of excess amounts of liquid to Cell or Filter

Concentrators will result in the fluid being “spun out” during centrifugation.

CAUTION - Do not place the cytocentrifuge on top of other equipment and do not place other objects or

equipment on top of the cytocentrifuge.

CAUTION - The cover interlock by-pass is for emergency use only. Do not attempt to operate the

Cytofuge 12 while the latch release tool is inserted in the housing. Misuse of the interlock bypass may

expose the operator to unsafe conditions.

CAUTION - Before using any cleaning or decontamination methods except those recommended,

operators should check with customer service that the proposed method will not damage the

equipment.

CAUTION - The rotor (CFRT-12) is designed for many years of service but, depending on the

frequency of use, the rotor components may develop cracks over time or if dropped on a hard surface.

Inspect the rotor for damage or cracks before each use. If damage is found discontinue use and

contact customer service or your distributor for a replacement.

CAUTION - Do not lift the rotor (CFRT-12) by the locking button - the lid may release allowing the rotor

bowl to fall. Always lift the rotor by the black center grip.

CAUTION - The rotor (CFRT-12) is intended for use with biological samples only. Do Not place

flammable or reactive substances in the rotor.

CAUTION - While in the resting position, the rotor is designed to maintain the Concentrators at an

angle to prevent fluid from contacting the slide and/or the filter card prior to spinning. Keep the rotor

level during transport to and from the cytocentrifuge to ensure proper concentrator angle.

CAUTION - Do not defeat any safety interlocks while checking the rotor speed. Bypassing interlocks

could result in personal injury.

CAUTION - Unplug the cytocentrifuge from the wall outlet before cleaning or performing maintenance.

iii

CAUTION – Do not lean, or place materials, on the cytocentrifuge.

CAUTION - Follow Universal Precautions with all biological specimens, regardless of whether the

specimen is known to contain an infectious agent. Bioseals cannot be relied upon as a sole safeguard

against contamination by pathogens. Always load and unload the rotor under a biological safety hood.

(See references).

CAUTION – The seals in the removable rotor are intended to stop gross fluid leakage only. These

seals should not be relied upon for protection against biohazardous contamination.

Please use the system as intended. Improper use of the Cytofuge

12 Cytocentrifuge and/or its

accessories may cause personal injury, damage to the system, inaccurate results, or potentially nullify

warranties.

The Cytofuge

12 Cytocentrifuge System, and its associated components and consumables are covered

under U.S. Patent Nos. 5,480,484 & 5,679,154.

Section 1

Unpacking and Installation

Inspect Packaging

The Cytofuge

12 Cytocentrifuge and its accessories are delivered in a single carton. If the cytocentrifuge or

accessories have been damaged in transport, please inform your carrier immediately.

NOTE: Save the shipping carton and packing materials to facilitate return should service be required.

Verify Contents

The package contains:

One Cytofuge

12 Cytocentrifuge (CF12)

One cytocentrifuge rotor (CFRT-12)

One Accesory Carton

The Accesory Carton consists of:

One line cord (for use in North America only).

One Operator’s Manual CD

One latch release tool

3-well Cell Concentrators and 3-well gaskets

pkg of 4, (CC03)

1-well Cell Concentrators and 1-well gaskets

pkg of 4, (CC01)

Stainless Steel Clips for Cell Concentrators

pkg of 8, (CLIP)

Backing Plates for Cell Concentrators

pkg of 4, (BP01)

Filter Concentrators

2 bags of 4 each (FF01)

Stainless Steel Clips for Filter Concentrators

3 pkgs of 4, (FFCL)

Install

o Place the cytocentrifuge on a solid level surface suitable for operation of laboratory instrumentation.

CAUTION - Do not place the cytocentrifuge on top of other equipment and do not place other

objects or equipment on top of the cytocentrifuge.

o Position the cytocentrifuge out of direct sunlight and away from sources of intense heat or cold.

Improper conditions can cause damage to the housing, rendering unit unsafe to operate.

o See the Appendix for the acceptable range of operating temperature and humidity.

o Remove the foam shipping retainer around the rotor before operating the cytocentrifuge.

WARNING - Maintain a 300 mm (11.8 inches) clearance envelope around cytocentrifuge during

operation. Do not deposit any potentially hazardous materials within the CLEARANCE ENVELOPE.

Connect Power

o Plug the cytocentrifuge into a grounded outlet supplying 100-240 V @ 50-60 Hz.

o Turn on the power switch (located on the rear panel, above the line cord connector).

o When the Cytofuge 12 completes the power-on check it will give a two-beep ready signal. Press the

STOP/OPEN button to release the latch and open cover.

WARNING - Do not use the supplied line cord outside of North America. Use a power cord with a

grounded male connector suitable for the power outlet being used, which is rated at 3.0 Amps or

more and has an IEC320/CEE22 female connector to attach to the cytocentrifuge.

Section 2

System Overview

Principle and Intended Use

For in vitro diagnostic use in concentrating cells onto a microscope slide.

The Cytofuge

12 Cytocentrifuge is a microprocessor-controlled cell preparation system that uses

centrifugal force to deposit cells onto microscope slides. Samples are centrifuged in reusable or

disposable gasket-sealed chambers (Cell Concentrators), or in disposable chambers (Filter

Concentrators) that include a filter material to absorb and capture suspension fluid during centrifugation.

A dual row 12-position rotor with transparent lid is supplied as standard equipment. The system is

designed to be operated on a laboratory bench or, if potentially biohazardous samples are processed, in

a biological safety cabinet. A cover interlock system prevents operation until the cytocentrifuge cover is

securely latched, and prevents access to spinning parts until the rotor has come to a complete stop.

Speeds from 200 rpm to 2000 rpm and cycle times from 1 minute to 99 minutes may be selected. The

system features three acceleration rates and dynamic braking to protect delicate samples.

Symbols and Definitions

Product/Reference Number

Indicates the Iris Sample Processing

product/catalog number

Caution

Statement of caution/warning: Read

carefully.

Biohazard

Indicates potential biohazard

EC Representative

European Community Authorized

Representative

For in vitro diagnostic use

Indicates for use only as in-vitro diagnostic

device

Date of manufacture

Indicates instrument manufacturing date

Serial Number

Indicates instrument serial number

Consult Instructions

Refers operator to the instruction manual

for additional information

European Community

Compliance to IVD Directive

Agency

Listed device with Intertek testing agency

Fuse

Rating: (located on serial number label,

replace with same value and type)

Single Phase Alternating Current

IVD

Operator Controls

Start

Initiates the selected cycle

Stop/Open

Interrupts the cycle and releases the cover when the

rotor has stopped.

Cancels Programming Mode

Save

Enters Programming Mode to store speed, time and

acceleration settings

Acceleration Select

Depress to select acceleration profile - Low, Medium or

High

Increments program, speed or time

Down

Decrements program, speed or time

CONTROL PANEL

To change the cycle speed use the arrow keys to the right of the SPEED display. Each key press will

change the speed by 10 rpm. Press and hold the key for 2 seconds to change the rpm in 50 rpm

increments.

To change the cycle time use the arrow keys to the right of the TIME display. Each key press will change

the time by 1 minute. Press and hold the key for 2 seconds to change the time in 10 minute increments.

To change the Acceleration rate press the key until the desired rate (high, medium, or low) is illuminated.

To change the Program use the arrow keys to the right of the PROG display.

Error Indicators

Cytofuge

12 Cytocentrifuge error conditions are signaled by flashing codes appearing in the TIME

display on the front panel.

To clear an error condition, correct the problem and press the Stop/Open button.

Error Codes

Acceleration Error

Confirm no obstruction in bowl area, check

drive cone o-rings (see Section 5), contact

customer service.

Latch is not locked

Confirm cover is fully seated in closed

position.

Incorrect Speed

Remove and inspect rotor, check drive

cone o-rings (see Section 5), contact

customer service.

Cover is not closed

Confirm cover is fully seated in closed

position.

Out of balance

Confirm concentrators are balanced in

rotor and cytocentrifuge is on a level

surface.

Accessories

Product No.

Description

CC03

3-well Cell Concentrators and 3-well gaskets; Box of 10

CC01

1-well Cell Concentrators and 1-well gaskets; Box of 10

CLIP

Stainless Steel Clips for all Cell Concentrators; Bag of 10

GK01

Replacement Gasket for CC01 and CCR1; Box of 100

GK03

Replacement Gasket for CC03 and CCR3; Box of 100

CCR1

1-well Cell Concentrators, reusable, autoclavable; Box of 10

CCR3

3-well Cell Concentrators, reusable, autoclavable; Box of 10

BP01

Backing Plates for all Cell Concentrators; Box of 10

FF01

Filter Concentrators , Disposable; Box of 48

FF01-B

Filter Concentrators , Disposable; Box of 200 Filter Cards, 192 Concentrators

FFR1

Filter Concentrators, Reusable; Box of 200 Filter Cards, 20 Concentrators

FFCL

Stainless Steel Clips for Filter Concentrators; Box of 4

CFRT-12

Cytofuge 12 Rotor Assembly, replacement or spare, includes cover

CFRC-12

Cytofuge 12 Rotor Lid, replacement

Section 3

Sample Requirements/Preparation

Overview

The approximate cell concentration of the specimen should be established prior to slide preparation on

the Cytofuge 12. Samples containing higher than optimal cell concentration will produce slides with cells

too closely packed or overlapping. Samples containing too low a cell concentration will yield slides where

cells are difficult to find, count, or examine. The following is a general guideline for sample concentration

based on an average cell diameter of 10 µm:

Sample Concentration

Recommendation

500 - 1500 cells/µL*

Use sample as is

< 500 cells/µL

Pre-concentrate sample

> 1500 cells/µL

Dilute sample

* In samples known for low cell populations (e.g. CSF), 50-100 cell/µL will produce acceptable results.

CAUTION – Follow Universal Precautions with all biological specimens, regardless of whether a

specimen is known to contain an infectious agent. Bioseals cannot be relied upon as a sole

safeguard against contamination by pathogens. Always load and unload the rotor under a biological

safety hood (See references).

Sample Volumes

Use the following guidelines for sample volumes in each of the Cytofuge concentrators:

Max. Volume Range

Optimum Volume Range

Filter Concentrator

50 to 300 µL

100 to 300 µL

One-well Cell Concentrator

300 to 1600 µL

400 to 800 µL

Three-well Cell Concentrator

50 to 400 µL

100 to 200 µL

Pre-concentration

For best results, samples low in cellular content should be pre-concentrated. For example, if the original

sample contains about 100 cells/µL, a 10x pre-concentration will provide the 1,000 cells/µL, which is

recommended for the Cytofuge 12.

To pre-concentrate a sample,

1. Transfer 10 mL of sample to a conical polypropylene centrifuge tube.

2. Spin the sample at 1000-1500 xg for 10-15 minutes in a conventional centrifuge.

3. Decant 9 mL of cell-free supernatant.

4. Mix the cell pellet and remaining supernatant by vortexing or agitation of the tube.

5. Pipette appropriate volume of the concentrated sample to Cytofuge concentrators.

Dilution

For best results, dilute samples that have an extremely high cell density. For most applications, buffered

saline or standard tissue culture media (e.g. Geys balanced salt solution) may be used as a diluent.

Adding a drop or two of bovine serum albumin (BSA) promotes cell adhesion to the microscope slide.

Section 4

Operating Instructions

Opening and Closing the Cover

An electronic operated cover interlock mechanism prevents operation if the cover is not completely

closed and locked, and prevents the cover from opening when the rotor is turning. An operating cycle can

be initiated only if the cover latch is engaged.

Cover Interlock By-pass

In case of power failure or malfunction, the Stop/Open button may not release the cover. In this event

the cover interlock mechanism can be released manually by inserting the manual latch release tool

supplied with the Cytofuge 12 into the small hole located in the front of the housing. Push the tool inward

to release the latch. Remove the tool before re-starting the cytocentrifuge.

CAUTION - The cover interlock by-pass is for emergency use only. Do not attempt to operate the

Cytofuge 12 while the latch release tool is inserted in the housing. Misuse of the interlock bypass

may expose the operator to unsafe conditions.

Using the Removable Rotor

The Cytofuge

12 Cytocentrifuge is shipped with the rotor installed and supported by a shipping

retainer. To prevent possible damage to the cytocentrifuge motor mount, it is important to remove the

rotor whenever the cytocentrifuge is moved or shipped to another location.

CAUTION - The rotor (CFRT-12) is intended for use with biological samples only. Do Not place

flammable or reactive substances in the rotor.

CAUTION - The rotor (CFRT-12) is designed for many years of service but, depending on the

frequency of use, the rotor components may develop cracks over time or if dropped on a hard

surface. Inspect the rotor for damage or cracks before each use. If damage is found discontinue

use and contact customer service or your distributor for a replacement.

To remove the rotor:

Open cytocentrifuge cover. Grasp rotor lid from the black

center grip and lift straight up and out of cytocentrifuge.

Never lift the rotor by the center button.

To open the rotor:

With rotor removed from instrument, pull up on the center

button to release lid.

Pull up on the black center grip to remove the lid.

To re-install rotor:

Place lid onto rotor. Press center button to lock lid in place.

Lift rotor by black center grip. Place rotor assembly onto cone located in center of instrument.

CAUTION - Do not lift the rotor (CFRT-12) by the locking button - the lid may release allowing the

rotor bowl to fall. Always lift the rotor by the black center grip.

Pull to release lid

Spinning Samples

CAUTION – Maintain a 300 mm (11.8 inches) clearance envelope around cytocentrifuge during

operation. Do not deposit any potentially hazardous materials within the clearance envelope.

CAUTION – Do not lean, or place materials, on the cytocentrifuge.

CAUTION – The seals in the removable rotor are intended to stop gross fluid leakage only. These

seals should not be relied upon for protection against biohazardous contamination.

CAUTION – Follow Universal Precautions with all biological specimens, regardless of whether the

specimen is known to contain an infectious agent. Bioseals cannot be relied upon as a sole

safeguard against contamination by pathogens. Always load and unload the rotor under a biological

safety hood.

1. Press Stop/Open button on front panel to release latch and open cover.

2. Remove rotor from cytocentrifuge and transport to a biological safety hood.

3. Remove the lid and load the concentrators into the rotor (concentrators must be equally

distributed and equally filled to properly balance rotor).

4. Slowly add sample to the bottom of the Filter Concentrator or Cell concentrator well. Avoid

getting droplets onto the walls of the funnel/well.

5. Install lid onto rotor, press center button to lock.

6. Place rotor into cytocentrifuge, assure rotor is fully seated onto cone in center of bowl.

7. Close cover and set spin cycle.

CAUTION – Do not overfill the concentrators. Addition of excess amounts of liquid to Cell or Filter

Concentrators will result in the fluid being “spun out” during centrifugation.

CAUTION – While in the resting position, the rotor is designed to maintain the Concentrators at an

angle to prevent fluid from contacting the slide and/or the filter card prior to spinning. Keep the rotor

level during transport to and from the cytocentrifuge to ensure proper concentrator angle.

Running a Cycle

1. Use the buttons on the front panel to select the desired acceleration, speed and time for the

cycle. Alternatively, select a program number to retrieve previously saved settings (See

Programming - pg 8). The table below lists the relative centrifugal force (RCF) at several

speeds.

2. Press start to begin cycle. The Cytofuge 12 will spin for the set time and automatically stop.

Note: Settings cannot be changed once the cycle begins.

Speed

(RPM)

RCF

(xg)

Speed

(RPM)

RCF

(xg)

200

1200

204

300

1300

240

400

1400

278

500

1500

319

600

1600

364

700

1700

410

800

1800

460

900

115

1900

513

1000

142

2000

568

1100

172

Note: Relative centrifugal force at other speeds may be calculated by:

RCF = 142 x (RPM/1000)

Cytofuge 12 operating parameters generally depend upon the size and specific gravity of the cells to

be concentrated onto the microscope slide. The operator should experiment with different settings to

achieve optimum performance for specific applications. The following are general guidelines:

Application

Speed Range (RPM)

Time Range

Cytology

400 to 650

2 to 4 minutes

Hematology

650 to 1500

4 to 8 minutes

Microbiology

1000 to 2000

4 to 10 minutes

For fragile cells, reduce speed.

For wet preparations, reduce time

For small particles (e.g. bacteria), increase speed and time

For specific application procedures, visit www.statspin.com to access Application Notes for the

Cytofuge 12 cytocentrifuge.

Unloading

1. Upon completion of the cycle, the rotor will decelerate to a complete stop, a signal beep will

sound, and the cover latch will automatically release.

2. Lift the cover and carefully remove the rotor and transport to a biological hood.

3. Remove the rotor lid to access the sample concentrators

Programming

The Cytofuge

12 Cytocentrifuge can store up to 24 user-defined programs. Each program allows

independent settings for speed, time and acceleration. When a program number is selected, the display

will automatically update to show the speed, time, and acceleration associated with that program.

1. Press the Save button once. The displays will flash indicating that the instrument is in programming

mode.

2. Select the program number to be stored by depressing the up or down arrow keys to the right of the

Program display.

Note: Program 00 contains the default instrument settings and cannot be changed.

Programming can be cancelled at any time by pressing the Stop/Open button. The previous values for

that program number will be retained.

3. Adjust the speed and time values in a similar manner and select the desired acceleration rate.

4. Press the Save button a second time to store the values under the selected program number and exit

programming mode.

Section 5

Maintenance

Overview

Iris Sample Processing recommends that instrument operators perform periodic inspections and

preventative maintenance on all instruments. Contact customer service or your distributor for optional

repair and calibration services offered for this instrument.

CAUTION - Unplug the cytocentrifuge from the wall outlet before cleaning or performing

maintenance.

WARNING - Inspect instrument for cracks or any physical damage to housing, cover, lid and rotor.

Damage may result in unsafe operation; discontinue use until repairs have been performed.

WARNING - Do not expose the cytocentrifuge or its rotor to strong or concentrated acids, bases or

oxidizing agents, aromatic or halogenated hydrocarbons, esters or ketones.

Cleaning

Clean the outside surfaces and switch overlay panel of the cytocentrifuge with a water-dampened cloth

and mild detergent. Remove rotor from instrument. Clean the inner surface or bowl with a mild detergent,

and if necessary, a disinfectant. Acceptable disinfectants are 70% alcohol or 10% bleach solution.

CAUTION - Before using any cleaning or decontamination methods except those recommended,

operators should check with customer service that the proposed method will not damage the

equipment.

General Cleaning

1. Unplug cytocentrifuge from the electrical outlet.

2. Open the cytocentrifuge cover and remove the rotor.

3. Clean liner and soiled external surfaces with a soft cloth dampened with a mild detergent.

4. Re-install rotor (see Removing and Installing the Rotor).

5. Plug into electrical outlet.

Rotor Disinfection

1. If necessary, remove rotor from the cytocentrifuge.

2. Remove lid from rotor bowl.

3. Remove center seal from rotor bowl.

4. Clean all parts with a soft cloth dampened with a mild detergent.

5. Soak all parts in a 10% bleach solution for 30 minutes.

6. Rinse the parts under running tap water or soak for 5 minutes.

7. Dry the parts with a clean absorbent paper towel or allow to air dry.

8. Replace the rotor seal, re-attach the lid and re-install the rotor in the Cytofuge 12.

Rotor Sterilization

1. If necessary, remove rotor from the cytocentrifuge.

2. Remove lid from rotor bowl.

3. Remove center seal from rotor bowl.

4. Clean all parts with a soft cloth dampened with a mild detergent or if heavily soiled, soak in mild detergent.

5. Place all parts into autoclave at 121 C for 15 minutes.

6. After sterilizing, replace rotor seal, re-attach lid and re-install the rotor in the Cytofuge 12.

Checking/Replacing Rotor Seal

Over time seals may become brittle and develop cracks. To ensure optimal rotor performance, check the

inner and outer seals of the rotor regularly for any tears or cracks. The center seal is user replaceable.

The outer seal is part of the rotor bowl – if this seal becomes worn or damaged a new rotor bowl should

be purchased.

Contact customer service or your distributor to purchase a rotor seal kit (P/N 01-006166-001), which

includes the center seal and drive cone o-rings, or a new rotor bowl (P/N 01-006168-001), if necessary.

To replace the center seal, simply pull the seal off of the rotor bowl and press on the seal as shown in the

figure.

Checking/Replacing Drive Cone O-rings

Over time the drive cone o-rings may become either worn or rough. If the o-rings need replacing, remove

the o-rings with a paperclip or tweezers and slide the new o-rings into the grooves. The o-rings are two

different sizes – the large o-ring (P/N 23-003019-137) fits the large diameter groove and the small o-ring

(P/N 23-003019-128) fits the smaller diameter groove. Both o-rings and the center seal are included in

the rotor seal kit (P/N 01-006166-001).

23-003019-128

23-003019-137

Checking the Rotor Speed

Rotor speeds (+5%) may be checked with a stroboscope or photoelectric tachometer.

1. Remove the rotor and locate the reflective patch located on the top of the drive cone.

Close the instrument cover.

2. Set the instrument to the desired speed.

3. Point the tachometer through the transparent cover at the reflective patch.

4. Press the Start button. Allow the instrument sufficient time to reach speed before making

a measurement.

If the instrument fails to maintain set speed within specified tolerance contact customer service

or your distributor.

CAUTION - Do not defeat any safety interlocks while checking the rotor speed. Bypassing

interlocks could result in personal injury.

Troubleshooting

Symptom

Solution

Error indicator flashing, unit will not spin

Refer to Error Code Table of manual to identify and correct problem.

Concentrators are loose and do not fit into rotor

Use only Cytofuge concentrators, other manufactures may look similar but

only Cytofuge concentrators may be used.

No display lights are on and unit will not spin

Main power switch is located on rear panel along with fuse holder. Check

switch position and, if necessary, condition of fuses.

Confirm line cord is plugged into a powered outlet.

Unit vibrates then shuts down

This instrument detected an imbalance and stopped operation. Clear the

error via keypad. Make sure concentrators are balanced within the rotor.

There is a crack in the top cover or housing

Discontinue operation. Contact Customer Service for repair options or a

list of authorized repair facilities.

Cover does not open

Confirm unit has power; press the Stop/Open button.

In the event of a power failure or malfunction, insert latch release tool into

the small hole on front panel. Cover will release and samples can be

removed.

Service

Refer all service to Iris Sample Processing or an Authorized Repair Facility.

Instrument fuse(s) are located on the rear panel. Remove the line cord to access the fuse holder.

Replace with fuses of the same type and rating.

2.5A 250V F 5 x 20 mm

Be sure to complete the Warranty Registration Card as directed.

Decontamination before returning for service:

Any instrument or accessory that has been exposed to blood or other biological materials must be

cleaned prior to shipment to the manufacturer or distributor for service. This decontamination is

required by Federal Law (Title 48 and 49 of the Federal Regulations) and in accordance with the

Environmental Protection Agency’s Regulations for Biohazard Waste Management. Iris Sample

Processing personnel cannot perform this decontamination. Refer to maintenance section for cleaning

and disinfection instructions.

Section 6

Appendix

Specifications

Product Name

Cytofuge

12 Cytocentrifuge

Product No.

CF12

Model No.

M802

Capacity

12 Filter and/or Cell concentrators

Programs

24 user configurable

Speed

Range

Accuracy

200-2000 rpm

± 5%

Time

Range

Accuracy

1-99 minutes

± 2 seconds

Acceleration

Range

LO (45-50 RPM/sec); MED (65-70 RPM/sec); HI (85-90 RPM/sec)

Deceleration

Fixed

< 1min

Electrical

100 – 240 VAC, 50/60Hz, 2.5 A

300 W

Physical

Height: 8.0“ (20.3 cm)

Width: 13.0” (33.0 cm)

Depth: 16.0” (40.6 cm)

Weight: 22 lbs. (10 Kg)

Environmental

For indoor use only

Operational Temperature

+5ºC to +40ºC

Relative Humidity

Maximum 80% for <31ºC,

Maximum 50% for 31-40ºC

Overvoltage Category

Storage/Transit Temperature

-10ºC to +50ºC

Altitude

2000m max

Pollution Degree

Specifications are subject to change without notice.

References

1. CLSI. “Protection of Laboratory Workers from Occupationally Acquired Infections; Approved

Guideline-Third Edition.” CLSI document M29-A3 [ISBN 1-56238-567-4]. CLSI, 940 West

Valley Rd, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2005.

2. CDC. Recommendations for Prevention of HIV Transmission in Health Care Settings. MMWR

(Suppl. No. 2S):2S-18S, 1987.

3. CDC. Updated: US Public Health Service Guidelines for the Management of Occupational

Exposures to HBV, HCV and HIV and Recommendations for Post Exposure Prophylaxis.

Appendix A and B. MMWR 50 (RR-11): 1-42, June 29, 2001.

55-006099-001 Rev A

A Division of IRIS International, Inc.

Westwood, MA 02090 USA

Tel: (781) 551-0100

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IRIS Cytofuge 12 User manual

IRIS Cytofuge 12 User manual

IRIS Cytofuge 12 User manual

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