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Thermo Fisher Scientific Preparation of RNA Ladders User guide

  • Hello! I've reviewed the user guide for preparing Thermo Fisher Scientific's RiboRuler RNA Ladders for electrophoresis. The guide provides detailed steps for both standard and ready-to-use ladders, including heating and chilling procedures before loading on the gel. It also notes the incompatibility with glyoxal/DMSO agarose gels. I'm ready to answer any questions you may have about using these RNA ladders.
  • How do I prepare RiboRuler RNA ladders?
    How do I prepare RiboRuler ready-to-use RNA ladders?
    Are these ladders suitable for glyoxal/DMSO agarose gel electrophoresis?
Protocol
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Preparation of RNA Ladders for
Electrophoresis
For RiboRuler™ RNA ladders:
• Mix1volumeofRNAladderand1volumeofthesupplied2XRNALoadingDye.
• Heatat70°Cfor10min.
• Chillonicefor3minutesandspindownpriortoloadingonagel.
• Load0.5µlofthepreparedladderforeverymmofgellanewidth(4µl/8mmlane).
For RiboRuler™ RNA ladders, ready-to-use:
• HeatRNAladdersat70°Cfor10min.
• Chillonicefor3minutesandspindownpriortoloadingonagel.
• Load0.5µloftheladderforeverymmofgellanewidth(4µl/8mmlane).
Note
Ladderspreparedasdescribedabovearenotsuitableforglyoxal/DMSOagarosegelelectrophoresis.
This protocol is for the Preparation of RNA Ladder for Electrophoresis
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