Thermo Fisher Scientific Preparation of RNA Ladders User guide

Protocol

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Preparation of RNA Ladders for

Electrophoresis

For RiboRuler™ RNA ladders:

• Mix1volumeofRNAladderand1volumeofthesupplied2XRNALoadingDye.

• Heatat70°Cfor10min.

• Chillonicefor3minutesandspindownpriortoloadingonagel.

• Load0.5µlofthepreparedladderforeverymmofgellanewidth(4µl/8mmlane).

For RiboRuler™ RNA ladders, ready-to-use:

• HeatRNAladdersat70°Cfor10min.

• Chillonicefor3minutesandspindownpriortoloadingonagel.

• Load0.5µloftheladderforeverymmofgellanewidth(4µl/8mmlane).

Note

Ladderspreparedasdescribedabovearenotsuitableforglyoxal/DMSOagarosegelelectrophoresis.

This protocol is for the Preparation of RNA Ladder for Electrophoresis